Table 1.

Summary of clinical and immunological phenotypes of IKZF-associated IEI

IKAROS deficiencyIKAROS-GOF diseaseHELIOS deficiencyAIOLOS deficiency
Mechanisms LOF, HI, DD, DN GOF LOF, altered protein interaction, DD LOF, DN, heteromeric interference, HI, DD 
Causative gene IKZF1 IKZF1 IKZF2 IKZF3 
Inheritance AD AD AD or AR AD 
Reported frequency 1–2% to 10% within CVID cohort (45, 46, 47) Unknown Unknown Unknown 
Immunological abnormalities 
 B cell phenotype B cell deficiency ∼ B lymphopenia (often progressive, mild in DD) Occasional B lymphopenia Occasional B lymphopenia ↓ memory B, occasional B lymphopenia 
 Immunoglobulin levels Agammaglobulinemia ∼ hypoimmunoglobulinemia Dysgammaglobulinemia Occasional hypoimmunoglobulinemia Agammaglobulinemia ∼ normal range 
 T cell phenotype Variable, ↓ memory T in DN ↑ CD4+ TEM and CD8 TEMRA, Th2 skewing, ↓ Treg CD4+ T and CD8+ T skewing toward memory. Occasional ↓ number or ↓ suppressive function of Treg Variable, ↓ naïve CD4+ T (other than DN), occasional CD4+ T skewing toward naïve in DN 
Clinical manifestations 
Infections ++ (mild in DD), +++ (DN) ++ ++, +++ (DN) 
Immune dysregulation ++ ++ ++ (HI and DD) 
Malignancy +++ 
Cytopenias − (occasional AIC) − (frequent AIC) 
Syndromic manifestation − − + (ICHAD syndrome) − 
IKAROS deficiencyIKAROS-GOF diseaseHELIOS deficiencyAIOLOS deficiency
Mechanisms LOF, HI, DD, DN GOF LOF, altered protein interaction, DD LOF, DN, heteromeric interference, HI, DD 
Causative gene IKZF1 IKZF1 IKZF2 IKZF3 
Inheritance AD AD AD or AR AD 
Reported frequency 1–2% to 10% within CVID cohort (45, 46, 47) Unknown Unknown Unknown 
Immunological abnormalities 
 B cell phenotype B cell deficiency ∼ B lymphopenia (often progressive, mild in DD) Occasional B lymphopenia Occasional B lymphopenia ↓ memory B, occasional B lymphopenia 
 Immunoglobulin levels Agammaglobulinemia ∼ hypoimmunoglobulinemia Dysgammaglobulinemia Occasional hypoimmunoglobulinemia Agammaglobulinemia ∼ normal range 
 T cell phenotype Variable, ↓ memory T in DN ↑ CD4+ TEM and CD8 TEMRA, Th2 skewing, ↓ Treg CD4+ T and CD8+ T skewing toward memory. Occasional ↓ number or ↓ suppressive function of Treg Variable, ↓ naïve CD4+ T (other than DN), occasional CD4+ T skewing toward naïve in DN 
Clinical manifestations 
Infections ++ (mild in DD), +++ (DN) ++ ++, +++ (DN) 
Immune dysregulation ++ ++ ++ (HI and DD) 
Malignancy +++ 
Cytopenias − (occasional AIC) − (frequent AIC) 
Syndromic manifestation − − + (ICHAD syndrome) − 

+, occasional; ++, frequent; +++, frequent and severe. AIC, autoimmune cytopenia; AD, autosomal dominant; AR, autosomal recessive; CID, combined immunodeficiency; TEM, effector memory T cells; TEMRA, effector memory T cells re-expressing CD45RA.

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