Primary and secondary antibodies
| Primary antibodies . | Source . | Identifier . |
|---|---|---|
| Immunofluorescence imaging | ||
| Acetylated tubulin (anti-mouse, 1:500) | Sigma-Aldrich | T6793 |
| ATP5 (ATP5A, ATP-synthase) (anti-mouse, 1:500) | Abcam | 14748 |
| BRP (Nc82) (anti-mouse, 1:250) | Developmental Studies Hybridoma Bank | Nc82 |
| BRP (last 200) (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Ullrich et al., 2015) | last200 (rb) |
| DAP160 (anti-rabbit, 1:500) | Gift from O. Shupliakov laboratory, Karolinska Institutet, Solna, Sweden (Winther et al., 2015) | N/A |
| DsRed (anti-rabbit, 1:500) | Clontech | 632496 |
| Fasciclin II (Fas2, anti-mouse, 1:50) | Developmental Studies Hybridoma Bank | 1D4 anti-fasciclin II |
| Futsch (anti-mouse, 1:250) | Developmental Studies Hybridoma Bank | 22C10 |
| GFP (anti-mouse, 1:500) | Invitrogen | A11120 |
| Fab1 Al488 (goat–anti-rabbit, 1:50) | Jackson ImmunoResearch | 111–547-008 |
| Fab1 Al647 (goat–anti-rabbit, 1:200) | Jackson ImmunoResearch | 111–607-008 |
| Liprin-α (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Depner et al., 2014) | C-term, 8111 |
| Myo15C1 (anti-rabbit, 1:25) | This study | rb 774 |
| Myo15C2 (anti-rabbit, 1:50) | This study | rb 15073 |
| Neuroglian (Nrg, anti-mouse, 1:50) | Developmental Studies Hybridoma Bank | BP 104 |
| Rab3 (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Turrel et al., 2024) | N/A |
| Rbsn-5 (anti-rabbit, 1:1,000) | Gift from A. Nakamura laboratory, RIKEN Center for Developmental Biology, Kobe, Japan (Tanaka and Nakamura, 2008) | N/A |
| RIM-BP (anti-guinea pig, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Bohme et al., 2016) | RBP gp |
| VGlut (anti-rabbit, 1:500) | Gift from H. Aberle laboratory, Heinrich-Heine-Universität, Düsseldorf, Germany (Mahr and Aberle, 2006) | C-term, VGlut |
| Western blots | ||
| ATP5A (ATP-synthase) (anti-mouse, 1:1000) | Abcam | 14748 |
| GFP (anti-mouse, 1:4,000) | Sigma-Aldrich | SAB4301795 |
| α-tubulin (anti-mouse, 1:100,000) | Sigma-Aldrich | T5168 |
| Secondary antibodies | ||
| Immunofluorescence imaging | ||
| Alexa 488 (anti-rabbit, 1:500) | Invitrogen | A-11008 |
| Alexa 488 (anti-mouse, 1:500) | Invitrogen | A-11001 |
| Alexa 488 (anti-guinea pig 1:500) | Invitrogen | A-11073 |
| Alexa 594 (anti-rabbit, 1:500 for STED) | Invitrogen | A32754 |
| Alexa 594 (anti-mouse, 1:500 for STED) | Invitrogen | A32742 |
| Atto 647N (anti-rabbit, 1:500 for STED) | Sigma-Aldrich | 40839 |
| Atto 647N (anti-mouse, 1:500 for STED) | Sigma-Aldrich | 50185 |
| Cy3 (anti-rabbit, 1:500) | Abcam | ab6939 |
| Cy3 (anti-mouse, 1:500) | Abcam | ab97035 |
| antiGFP StarRED (1:500 for STED) | Abberior | STRED |
| HRP-Alexa647 (conjugated antibody), (1:500) | Jackson ImmunoResearch | 123–605-021 |
| Western blots | ||
| HRP-coupled goat anti-mouse (1:5,000) | Jackson ImmunoResearch | #115-035-003 |
| HRP-coupled goat anti-rabbit (1:5,000) | Jackson ImmunoResearch | #111-035–144 |
| Primary antibodies . | Source . | Identifier . |
|---|---|---|
| Immunofluorescence imaging | ||
| Acetylated tubulin (anti-mouse, 1:500) | Sigma-Aldrich | T6793 |
| ATP5 (ATP5A, ATP-synthase) (anti-mouse, 1:500) | Abcam | 14748 |
| BRP (Nc82) (anti-mouse, 1:250) | Developmental Studies Hybridoma Bank | Nc82 |
| BRP (last 200) (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Ullrich et al., 2015) | last200 (rb) |
| DAP160 (anti-rabbit, 1:500) | Gift from O. Shupliakov laboratory, Karolinska Institutet, Solna, Sweden (Winther et al., 2015) | N/A |
| DsRed (anti-rabbit, 1:500) | Clontech | 632496 |
| Fasciclin II (Fas2, anti-mouse, 1:50) | Developmental Studies Hybridoma Bank | 1D4 anti-fasciclin II |
| Futsch (anti-mouse, 1:250) | Developmental Studies Hybridoma Bank | 22C10 |
| GFP (anti-mouse, 1:500) | Invitrogen | A11120 |
| Fab1 Al488 (goat–anti-rabbit, 1:50) | Jackson ImmunoResearch | 111–547-008 |
| Fab1 Al647 (goat–anti-rabbit, 1:200) | Jackson ImmunoResearch | 111–607-008 |
| Liprin-α (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Depner et al., 2014) | C-term, 8111 |
| Myo15C1 (anti-rabbit, 1:25) | This study | rb 774 |
| Myo15C2 (anti-rabbit, 1:50) | This study | rb 15073 |
| Neuroglian (Nrg, anti-mouse, 1:50) | Developmental Studies Hybridoma Bank | BP 104 |
| Rab3 (anti-rabbit, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Turrel et al., 2024) | N/A |
| Rbsn-5 (anti-rabbit, 1:1,000) | Gift from A. Nakamura laboratory, RIKEN Center for Developmental Biology, Kobe, Japan (Tanaka and Nakamura, 2008) | N/A |
| RIM-BP (anti-guinea pig, 1:500) | S.J. Sigrist laboratory, Freie Universität Berlin, Germany (Bohme et al., 2016) | RBP gp |
| VGlut (anti-rabbit, 1:500) | Gift from H. Aberle laboratory, Heinrich-Heine-Universität, Düsseldorf, Germany (Mahr and Aberle, 2006) | C-term, VGlut |
| Western blots | ||
| ATP5A (ATP-synthase) (anti-mouse, 1:1000) | Abcam | 14748 |
| GFP (anti-mouse, 1:4,000) | Sigma-Aldrich | SAB4301795 |
| α-tubulin (anti-mouse, 1:100,000) | Sigma-Aldrich | T5168 |
| Secondary antibodies | ||
| Immunofluorescence imaging | ||
| Alexa 488 (anti-rabbit, 1:500) | Invitrogen | A-11008 |
| Alexa 488 (anti-mouse, 1:500) | Invitrogen | A-11001 |
| Alexa 488 (anti-guinea pig 1:500) | Invitrogen | A-11073 |
| Alexa 594 (anti-rabbit, 1:500 for STED) | Invitrogen | A32754 |
| Alexa 594 (anti-mouse, 1:500 for STED) | Invitrogen | A32742 |
| Atto 647N (anti-rabbit, 1:500 for STED) | Sigma-Aldrich | 40839 |
| Atto 647N (anti-mouse, 1:500 for STED) | Sigma-Aldrich | 50185 |
| Cy3 (anti-rabbit, 1:500) | Abcam | ab6939 |
| Cy3 (anti-mouse, 1:500) | Abcam | ab97035 |
| antiGFP StarRED (1:500 for STED) | Abberior | STRED |
| HRP-Alexa647 (conjugated antibody), (1:500) | Jackson ImmunoResearch | 123–605-021 |
| Western blots | ||
| HRP-coupled goat anti-mouse (1:5,000) | Jackson ImmunoResearch | #115-035-003 |
| HRP-coupled goat anti-rabbit (1:5,000) | Jackson ImmunoResearch | #111-035–144 |
VGlut, vesicular glutamate transporter 1.