Table 3. Crosses and genotypes used... | Rockefeller University Press

Table 3.

Crosses and genotypes used

Crosses	Experiment
ok6-Gal4, UAS-dicer2 x UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶, control: ok6-Gal4, UAS-dicer2 x w1118	RNAi-mediated knock-down
ok6-Gal4; UAS-Cas9 x Myo15 gRNA, control: ok6-Gal4; UAS-Cas9 x w1118	gRNA-mediated knock-down
ok6-Gal4 x UAS-Myo15-GFP/RFP, control: ok6-Gal4 x w1118	Overexpression (NMJ and salivary gland)
Mutant: myo15-/myo15-control: Wild type (w1118), elaV-Gal4 x UAS-Myo15-GFP, control: elaV-Gal4 x w1118	Western blots (larva), Western blots (adult brain)
ok6-Gal4 x UAS-actinGFP, ok6-Gal4, UAS-actinGFP × UAS-Myo15RFP, ok6-Gal4, UAS-actinGFP × UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶	Actin and Myo15 overexpression
ok6-Gal4; UAS-Myo15-RFP ×, UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶ ok6-Gal4; UAS-Myo15-RFP × w1118, control: ok6-Gal4 × w1118	Myo15 overexpression and RNAi simultaneously
Rescue: myo15-/FM7i, act-GFP; ok6-Gal4/II x myo15-/Y; UAS-Myo15^GFP/II, Mutant: myo15-/myo15-control: Wild type (w1118)	Presynaptic rescue
Tubulin-Gal80^ts; ok107-Gal4 x UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶ or UAS-Myo15-RNAi¹⁰⁴⁶⁰⁴	Conditional expression of RNAi in MB

Crosses	Experiment
ok6-Gal4, UAS-dicer2 x UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶, control: ok6-Gal4, UAS-dicer2 x w1118	RNAi-mediated knock-down
ok6-Gal4; UAS-Cas9 x Myo15 gRNA, control: ok6-Gal4; UAS-Cas9 x w1118	gRNA-mediated knock-down
ok6-Gal4 x UAS-Myo15-GFP/RFP, control: ok6-Gal4 x w1118	Overexpression (NMJ and salivary gland)
Mutant: myo15-/myo15-control: Wild type (w1118), elaV-Gal4 x UAS-Myo15-GFP, control: elaV-Gal4 x w1118	Western blots (larva), Western blots (adult brain)
ok6-Gal4 x UAS-actinGFP, ok6-Gal4, UAS-actinGFP × UAS-Myo15RFP, ok6-Gal4, UAS-actinGFP × UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶	Actin and Myo15 overexpression
ok6-Gal4; UAS-Myo15-RFP ×, UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶ ok6-Gal4; UAS-Myo15-RFP × w1118, control: ok6-Gal4 × w1118	Myo15 overexpression and RNAi simultaneously
Rescue: myo15-/FM7i, act-GFP; ok6-Gal4/II x myo15-/Y; UAS-Myo15^GFP/II, Mutant: myo15-/myo15-control: Wild type (w1118)	Presynaptic rescue
Tubulin-Gal80^ts; ok107-Gal4 x UAS-Myo15-RNAi¹⁰¹⁷²⁹; UAS-Myo15-RNAi³³⁴⁸⁶ or UAS-Myo15-RNAi¹⁰⁴⁶⁰⁴	Conditional expression of RNAi in MB