Information to include in the Materials and methods or figure legend
| Follow the journal's instructions for authors, and/or include the following: |
| • Manufacturer and model of microscope |
| • Objective lens magnification, NA, and correction for aberration (e.g., 60x 1.4 NA Plan-Apochromat) |
| • Fluorescence filter set manufacturer and part number and/or the transmission and bandwidth (e.g., 490/30) |
| • Illumination light source (including wavelength for laser illumination) |
| • Camera manufacturer and model |
| • Software program(s) and version |
| • Manufacturer and model of other acquisition hardware, including confocal, filter wheels, focus motors, motorized stage, shutters, etc. |
| • Image acquisition settings including exposure times, gain, and binning |
| • Other acquisition parameters, including focus step size (for z-series), time between images (for time lapse), etc. |
| • Description of image processing routine used to create figures |
| • Description of segmentation and image analysis routine, and method of validation |
| Follow the journal's instructions for authors, and/or include the following: |
| • Manufacturer and model of microscope |
| • Objective lens magnification, NA, and correction for aberration (e.g., 60x 1.4 NA Plan-Apochromat) |
| • Fluorescence filter set manufacturer and part number and/or the transmission and bandwidth (e.g., 490/30) |
| • Illumination light source (including wavelength for laser illumination) |
| • Camera manufacturer and model |
| • Software program(s) and version |
| • Manufacturer and model of other acquisition hardware, including confocal, filter wheels, focus motors, motorized stage, shutters, etc. |
| • Image acquisition settings including exposure times, gain, and binning |
| • Other acquisition parameters, including focus step size (for z-series), time between images (for time lapse), etc. |
| • Description of image processing routine used to create figures |
| • Description of segmentation and image analysis routine, and method of validation |