Table 1. PV loop parameters DMSO DN Low... | Rockefeller University Press

Table 1.

PV loop parameters

	DMSO	DN Low	DN High	OM Low	OM High
Δ HR (bpm)	7 ± 5	4 ± 5	−6 ± 6	1.1 ± 2.6	−10.9 ± 3.4
Δ ESV (µl)	−1.4 ± 0.6	−6.6 ± 0.5^{^a}	−12.6 ± 1.2^{^a}^,^{^b}	−7.2 ± 0.5^{^a}	−11.0 ± 1.1^{^a}^,^{^b}
Δ EDV (µl)	−0.08 ± 0.23	−1.86 ± 0.28	−4.8 ± 0.7^{^a}^,^{^b}	−2.3 ± 0.4^{^a}	−4.9 ± 0.7^{^a}^,^{^b}
Δ SV (µl)	1.0 ± 0.4	4.39 ± 0.35^{^a}	7.7 ± 0.8^{^a}^,^{^b}	4.1 ± 0.4^{^a}	7.6 ± 0.9^{^a}^,^{^b}
Δ EF (%)	3.5 ± 1.5	17.2 ± 2.1^{^a}	36.2 ± 2.9^{^a}^,^{^b}	16.2 ± 1.0^{^a}	30.7 ± 3.3^{^a}^,^{^b}
Δ SW (mmHg * μl)	146 ± 31	440 ± 50^{^a}	780 ± 90^{^a}^,^{^b}	400 ± 50	720 ± 80^{^a}^,^{^b}
Δ CO (µl/min)	550 ± 210	1,950 ± 270^{^a}	4,000 ± 500^{^a}^,^{^b}	2,010 ± 190^{^a}	3,500 ± 400^{^a}^,^{^b}
Δ PFR (µl/s)	15 ± 13	157 ± 25	390 ± 80^{^a}^,^{^b}	131 ± 28	300 ± 50^{^a}
Δ PER (µl/s)	38 ± 10	126 ± 14	280 ± 50^{^a}^,^{^b}	63 ± 15	83 ± 31^{^c}
Δ P_max (mmHg)	5.5 ± 1.4	5.2 ± 1.2	4.0 ± 2.1	1.0 ± 1.6	4.5 ± 1.6
Δ P_min (mmHg)	−0.15 ± 0.29	0.21 ± 0.27	0.15 ± 0.26	−0.12 ± 0.19	1.08 ± 0.26^{^a}^,^{^b}
Δ ESP (mmHg)	5.6 ± 1.4	2.8 ± 1.9	−1.4 ± 2.7	−1.5 ± 2.4	2.0 ± 1.9
Δ EDP (mmHg)	−0.19 ± 0.33	−0.2 ± 0.4	−0.44 ± 0.24	−0.41 ± 0.27	−0.09 ± 0.26
Δ dP/dt_max (mmHg/s)	520 ± 170	1,780 ± 90^{^a}	3,400 ± 500^{^a}^,^{^b}	1,830 ± 160^{^a}	3,120 ± 320^{^a}^,^{^b}
Δ dP/dt_min (mmHg/s)	−1,080 ± 290	−1,280 ± 200	−200 ± 400	−540 ± 270	30 ± 230
Δ Tau (ms)	−0.39 ± 0.21	0.10 ± 0.08	1.48 ± 0.12^{^a}^,^{^b}	0.43 ± 0.10^{^a}	2.72 ± 0.25^{^a}^,^{^b}^,^{^c}
Δ Systolic duration (ms)	−1.0 ± 0.7	1.0 ± 0.5	6.5 ± 1.3^{^a}^,^{^b}	3.4 ± 0.5^{^a}	11.1 ± 1.0^{^a}^,^{^b}^,^{^c}
Δ Systolic duration (% CC)	−0.09 ± 0.27	1.06 ± 0.22	4.1 ± 0.7^{^a}^,^{^b}	2.8 ± 0.4^{^a}^,^{^c}	7.3 ± 0.4^{^a}^,^{^b}^,^{^c}
Δ Diastolic duration (ms)	−1.1 ± 1.0	−1.8 ± 0.9	−4.0 ± 1.1	−3.5 ± 0.6	−8.0 ± 0.8^{^a}^,^{^b}^,^{^c}
Δ Diastolic duration (% CC)	0.09 ± 0.27	−1.06 ± 0.22	−4.1 ± 0.7^{^a}^,^{^b}	−2.8 ± 0.4^{^a}^,^{^c}	−7.3 ± 0.4^{^a}^,^{^b}^,^{^c}
Δ S/D	−0.003 ± 0.009	0.035 ± 0.007	0.144 ± 0.025^{^a}^,^{^b}	0.099 ± 0.015^{^a}^,^{^c}	0.264 ± 0.015^{^a}^,^{^b}^,^{^c}
Δ ESPVR slope (mmHg/μl)	0.34 ± 0.14	−1.4 ± 0.5^{^a}	−1.40 ± 0.28^{^a}	−2.25 ± 0.32^{^a}	−1.7 ± 0.6^{^a}
Δ ESPVR intercept (mmHg)	5.7 ± 2.2	49 ± 9^{^a}	66 ± 10^{^a}	60 ± 10^{^a}	58 ± 17^{^a}
Δ dP/dt_max-EDV slope (mmHg/s/μl)	0 ± 7	−9 ± 10	−83 ± 17^{^a}^,^{^b}	−6 ± 12	−83 ± 29^{^a}^,^{^b}
Δ dP/dt_max-EDV intercept (mmHg/s)	590 ± 250	1,900 ± 500	6,300 ± 400^{^a}^,^{^b}	2,930 ± 340^{^a}	5,400 ± 700^{^a}^,^{^b}
Δ EDPVR (1/μl)	0.027 ± 0.022	0.019 ± 0.020	−0.036 ± 0.015	−0.030 ± 0.014	−0.041 ± 0.018

	DMSO	DN Low	DN High	OM Low	OM High
Δ HR (bpm)	7 ± 5	4 ± 5	−6 ± 6	1.1 ± 2.6	−10.9 ± 3.4
Δ ESV (µl)	−1.4 ± 0.6	−6.6 ± 0.5^a	−12.6 ± 1.2^a^,^b	−7.2 ± 0.5^a	−11.0 ± 1.1^a^,^b
Δ EDV (µl)	−0.08 ± 0.23	−1.86 ± 0.28	−4.8 ± 0.7^a^,^b	−2.3 ± 0.4^a	−4.9 ± 0.7^a^,^b
Δ SV (µl)	1.0 ± 0.4	4.39 ± 0.35^a	7.7 ± 0.8^a^,^b	4.1 ± 0.4^a	7.6 ± 0.9^a^,^b
Δ EF (%)	3.5 ± 1.5	17.2 ± 2.1^a	36.2 ± 2.9^a^,^b	16.2 ± 1.0^a	30.7 ± 3.3^a^,^b
Δ SW (mmHg * μl)	146 ± 31	440 ± 50^a	780 ± 90^a^,^b	400 ± 50	720 ± 80^a^,^b
Δ CO (µl/min)	550 ± 210	1,950 ± 270^a	4,000 ± 500^a^,^b	2,010 ± 190^a	3,500 ± 400^a^,^b
Δ PFR (µl/s)	15 ± 13	157 ± 25	390 ± 80^a^,^b	131 ± 28	300 ± 50^a
Δ PER (µl/s)	38 ± 10	126 ± 14	280 ± 50^a^,^b	63 ± 15	83 ± 31^c
Δ P_max (mmHg)	5.5 ± 1.4	5.2 ± 1.2	4.0 ± 2.1	1.0 ± 1.6	4.5 ± 1.6
Δ P_min (mmHg)	−0.15 ± 0.29	0.21 ± 0.27	0.15 ± 0.26	−0.12 ± 0.19	1.08 ± 0.26^a^,^b
Δ ESP (mmHg)	5.6 ± 1.4	2.8 ± 1.9	−1.4 ± 2.7	−1.5 ± 2.4	2.0 ± 1.9
Δ EDP (mmHg)	−0.19 ± 0.33	−0.2 ± 0.4	−0.44 ± 0.24	−0.41 ± 0.27	−0.09 ± 0.26
Δ dP/dt_max (mmHg/s)	520 ± 170	1,780 ± 90^a	3,400 ± 500^a^,^b	1,830 ± 160^a	3,120 ± 320^a^,^b
Δ dP/dt_min (mmHg/s)	−1,080 ± 290	−1,280 ± 200	−200 ± 400	−540 ± 270	30 ± 230
Δ Tau (ms)	−0.39 ± 0.21	0.10 ± 0.08	1.48 ± 0.12^a^,^b	0.43 ± 0.10^a	2.72 ± 0.25^a^,^b^,^c
Δ Systolic duration (ms)	−1.0 ± 0.7	1.0 ± 0.5	6.5 ± 1.3^a^,^b	3.4 ± 0.5^a	11.1 ± 1.0^a^,^b^,^c
Δ Systolic duration (% CC)	−0.09 ± 0.27	1.06 ± 0.22	4.1 ± 0.7^a^,^b	2.8 ± 0.4^a^,^c	7.3 ± 0.4^a^,^b^,^c
Δ Diastolic duration (ms)	−1.1 ± 1.0	−1.8 ± 0.9	−4.0 ± 1.1	−3.5 ± 0.6	−8.0 ± 0.8^a^,^b^,^c
Δ Diastolic duration (% CC)	0.09 ± 0.27	−1.06 ± 0.22	−4.1 ± 0.7^a^,^b	−2.8 ± 0.4^a^,^c	−7.3 ± 0.4^a^,^b^,^c
Δ S/D	−0.003 ± 0.009	0.035 ± 0.007	0.144 ± 0.025^a^,^b	0.099 ± 0.015^a^,^c	0.264 ± 0.015^a^,^b^,^c
Δ ESPVR slope (mmHg/μl)	0.34 ± 0.14	−1.4 ± 0.5^a	−1.40 ± 0.28^a	−2.25 ± 0.32^a	−1.7 ± 0.6^a
Δ ESPVR intercept (mmHg)	5.7 ± 2.2	49 ± 9^a	66 ± 10^a	60 ± 10^a	58 ± 17^a
Δ dP/dt_max-EDV slope (mmHg/s/μl)	0 ± 7	−9 ± 10	−83 ± 17^a^,^b	−6 ± 12	−83 ± 29^a^,^b
Δ dP/dt_max-EDV intercept (mmHg/s)	590 ± 250	1,900 ± 500	6,300 ± 400^a^,^b	2,930 ± 340^a	5,400 ± 700^a^,^b
Δ EDPVR (1/μl)	0.027 ± 0.022	0.019 ± 0.020	−0.036 ± 0.015	−0.030 ± 0.014	−0.041 ± 0.018

Absolute change (∆) in LV PV loop parameters 1 min after infusion of either vehicle control (DMSO), DN at 3 mg/kg (DN Low), DN at 6 mg/kg (DN High), OM at 0.75 mg/kg (OM Low), or OM at 1.5 mg/kg (OM High). N = 7–11 mice/group.

Significance is defined as P < 0.05 for all comparisons as determined by ANOVA and Tukey’s HSD. HR, heart rate; ESV, end-systolic volume; EDV, end-diastolic volume; SV, stroke volume; EF, ejection fraction; SW, stroke work; CO, cardiac output; PFR, peak filling rate; PER, peak ejection rate; P_max, maximum LV pressure; P_min, minimum LV pressure; ESP, end-systolic pressure; EDP, end-diastolic pressure; dP/dt_max, maximum rate of LV pressure change; dP/dt_min, minimum rate of LV pressure change; Tau, the time constant of LV pressure relaxation; % CC, percent cardiac cycle; S/D, the ratio of systolic to diastolic duration; ESPVR, end-systolic pressure–volume relationship; EDPVR, exponent of the end-diastolic pressure–volume relationship.

Significant difference when compared to the DMSO group.

Significant difference when compared to the low dose of the same drug.

Significant difference when compared to the corresponding dose of DN.

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