Essential residues in DJ-1 and their proposed contribution to the reaction mechanism
| Essential residues in DJ-1 . | Proposed contribution to the cPGA hydrolysis . |
|---|---|
| E15 | Formation of the cPGA-binding pocket |
| E18 | Formation of a hydrogen bond with the hydroxy group of cPGA |
| R28 (protomer) | Determination of the side chain arrangement of E18 |
| G74 | Stabilization of tetrahedral intermediate by oxyanion hole formation |
| G75 | Stabilization of tetrahedral intermediate by oxyanion hole formation |
| C106 | Nucleophilic cysteine that attacks the cPGA carbonyl group to form the tetrahedral intermediate |
| A107 (backbone nitrogen) | Formation of a hydrogen bond with oxygen atoms on the carbonyl group of cPGA |
| P158 | Formation of the cPGA-binding pocket |
| Essential residues in DJ-1 . | Proposed contribution to the cPGA hydrolysis . |
|---|---|
| E15 | Formation of the cPGA-binding pocket |
| E18 | Formation of a hydrogen bond with the hydroxy group of cPGA |
| R28 (protomer) | Determination of the side chain arrangement of E18 |
| G74 | Stabilization of tetrahedral intermediate by oxyanion hole formation |
| G75 | Stabilization of tetrahedral intermediate by oxyanion hole formation |
| C106 | Nucleophilic cysteine that attacks the cPGA carbonyl group to form the tetrahedral intermediate |
| A107 (backbone nitrogen) | Formation of a hydrogen bond with oxygen atoms on the carbonyl group of cPGA |
| P158 | Formation of the cPGA-binding pocket |