Table 5.

Gene and primer sequences used for the Y1H experiment

GenePrimersAnnealing temperature
Crq FP-hind3 5′-CCC​AAG​CTT​TGC​TTA​CAG​TGC​TGT​TAC​AG-3′ 61.5°C 
Crq RP-kpn1 5′-CGG​GGT​ACC​GGT​GGA​TAC​GTA​GGA​TTC​C-3′ 
E93gibson-For 5′-AGT​ACC​CAT​ACG​ACG​TAC​CAG​ATT​ACG​CTC​AGA​TGG​CCG​ATT​GTT​CAT​ATG​T-3′ 64°C 
E93gibson_Rev 5′-TTC​AGT​ATC​TAC​GAT​TCA​TCT​GCA​GCT​CGA​CTA​TAA​CTG​ATC​CGC​CTG​GT-3′ 
GenePrimersAnnealing temperature
Crq FP-hind3 5′-CCC​AAG​CTT​TGC​TTA​CAG​TGC​TGT​TAC​AG-3′ 61.5°C 
Crq RP-kpn1 5′-CGG​GGT​ACC​GGT​GGA​TAC​GTA​GGA​TTC​C-3′ 
E93gibson-For 5′-AGT​ACC​CAT​ACG​ACG​TAC​CAG​ATT​ACG​CTC​AGA​TGG​CCG​ATT​GTT​CAT​ATG​T-3′ 64°C 
E93gibson_Rev 5′-TTC​AGT​ATC​TAC​GAT​TCA​TCT​GCA​GCT​CGA​CTA​TAA​CTG​ATC​CGC​CTG​GT-3′ 
View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal