Table 4.

List of primers sequences for semi-quantitative RT-PCR

GenePrimersAnnealing temperature
Crq Left 5′-CCG​AGA​CAA​AGC​ACC​CAT​TT-3′ 56°C 
Crq Right 5′-CGT​CAT​CGG​GCT​CAA​TCA​TC-3′ 
Draper Left 5′-CCT​GCC​CAA​CAA​TAT​GCG​TT-3′ 56°C 
Draper Right 5′-TCC​TTG​TAG​CCC​TCC​TTG​TG-3′ 
PGRP-LB Left 5′-AAG​AAC​CTG​ATC​GCC​TTT​GG-3′ 56°C 
PGRP-LB Right 5′-GGA​TTG​GTG​CGG​CTT​TTG​T-3′ 
Tub84b Left 5′-CCT​TCG​TCC​ACT​GGT​ACG​TT-3′ 59°C 
Tub84b Right 5′-GGC​GTG​ACG​CTT​AGT​ACT​C-3′ 
Serpent Left 5′-GCA​ACA​GCT​TCC​ACA​GTA​CC-3′ 57°C 
Serpent Right 5′-GTT​TGA​GTT​GGG​CGA​CAT​GT-3′ 
Eip93F Left 5′-GCA​ATG​CCC​TCA​AGA​ACC​AA-3′ 53°C 
Eip93F Left 5′-CTA​CCG​TTG​CTG​CCA​TTA​CG-3′ 
GenePrimersAnnealing temperature
Crq Left 5′-CCG​AGA​CAA​AGC​ACC​CAT​TT-3′ 56°C 
Crq Right 5′-CGT​CAT​CGG​GCT​CAA​TCA​TC-3′ 
Draper Left 5′-CCT​GCC​CAA​CAA​TAT​GCG​TT-3′ 56°C 
Draper Right 5′-TCC​TTG​TAG​CCC​TCC​TTG​TG-3′ 
PGRP-LB Left 5′-AAG​AAC​CTG​ATC​GCC​TTT​GG-3′ 56°C 
PGRP-LB Right 5′-GGA​TTG​GTG​CGG​CTT​TTG​T-3′ 
Tub84b Left 5′-CCT​TCG​TCC​ACT​GGT​ACG​TT-3′ 59°C 
Tub84b Right 5′-GGC​GTG​ACG​CTT​AGT​ACT​C-3′ 
Serpent Left 5′-GCA​ACA​GCT​TCC​ACA​GTA​CC-3′ 57°C 
Serpent Right 5′-GTT​TGA​GTT​GGG​CGA​CAT​GT-3′ 
Eip93F Left 5′-GCA​ATG​CCC​TCA​AGA​ACC​AA-3′ 53°C 
Eip93F Left 5′-CTA​CCG​TTG​CTG​CCA​TTA​CG-3′ 

Tub84b was used as a housekeeping control.

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