Table IV.

Primers used in this study

Primer Name Sequence 
RSAPex25-1 5′-GGGGACAACT­TTGTATAGAAA­AGTTGCAAAGT­CTGGATGGAGG­CTTCATCTC-3′ 
RSAPex25-2 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGAGCGTG­GCATGCGGTTC­ATAGAAAC-3′ 
RSAPex25-3 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGGAGTCT­CTGCTCGCGTA­CAAGATC-3′ 
RSAPex25-4 5′-GGGGACAACT­TTGTATAATAA­AGTTGACTTGG­AGCTGCTGTGC­TTGTATG-3′ 
attB1-Ptef1-forward 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTGAT­CCCCCACACAC­CATAGCTTC-3′ 
attB2-Ttef1-reverse 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTGCT­CGTTTTCGACA­CTGGATGG-3′ 
RSAPex25-5 5′-CTGGATGGAGGCTTCATCTC-3′ 
RSAPex25-6 5′-GGAGCTGCTGTGCTTGTATG-3′ 
RSAPex11C-1 5′-GGGGACAACT­TTGTATAGAAA­AGTTGTACCAG­AGCTCATGTGC­TGTTCCAG-3′ 
RSAPex11C-2 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGAAGATC­CATAACAGACG­GTCGACAG-3′ 
RSAPex11C-3 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGCAACTG­GACGCACCTTG­AAAAGTC-3′ 
RSAPex11C-4 5′-GGGGACAACT­TTGTATAATAA­AGTTGGAAAGC­CGGTCTATCAG­GTCAAGC-3′ 
RSAPex11C-5 5′-ACCAGAGCTCATGTGCTGTTCCAG-3′ 
RSAPex11C-6 5′-GAAAGCCGGTCTATCAGGTCAAGC-3′ 
RSAPex11Cfusfw 5′-CCCAAGCTTT­GCTGCGACTGC­TAGCCAATCCCA-3′ 
RSAPex11Cfusrev 5′-AGATCTTCCA­ACAAGCTGGCG­CAACTGTGCAGA-3′ 
BB-JK-037 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTGTA­TGTCGTTTAAC­GACGATCTTTA­TAGGG-3′ 
BB-JK-038 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTTAT­TCAGGCAGGGA­TTTAGCTCCTT­TTCCG-3′ 
RSAatt PAOX F 5′-GGGGACAACT­TTGTATAGAAA­AGTTGGATCTC­GACGCGGAGAA­CGATC-3′ 
RSAattBIPrev 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGGAAACT­GCTGTGTTGTT­AGTG-3′ 
RSAattB1Pex3fwA 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTTGT­TCCAATATTGT­AGAGATCTT-3′ 
RSAattB2Pex3rev 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTAAG­CATCGAAATTA­GAGTAGAC-3′ 
RSA10fw 5′-GAAGATCTATGGTGAGCAAGGGCGAGGAG-3′ 
RSA11rev 5′-GCGTGTCGAC­TTACTTGTACA­GCTCGTCCATGCC-3′ 
RSA12Fw 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGCCATGG­TGAGCAAGGGC­GAGGAG-3′ 
RSA13Rev 5′-GGGGACAACT­TTGTATAATAA­AGTTGCGATCT­GAACCTCGACT­TTCTG-3′ 
att PAOX F 5′-GGGGACAACT­TTGTATAGAAA­AGTTGGATCTC­GACGCGGAGAA­CGATC-3′ 
att PAOX R 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGGTTTTT­GTACTTTAGAT­TGATGTCACC-3′ 
KN18 5′-CCCAAGCTTG­GATCCATGTTA­ACTTTCAATAA­GTC-3′ 
KN19 5′-GGGAAGCTTA­GATCTAAACTG­CTGTGTTGTTA­GTGGG-3′ 
KN14 5′-CCCCTCGAGA­ACCTGTACTTC­CAGTCGAGATC­TGTGAGCAAGG­GCGAGGAGC-3′ 
KN17 5′-GGGGTCGACT­TACAGCTCGTC­GTGAAGCTTGT­ACAGCTCG-3′ 
RSAPex11fwbamhi 5′-CGCGGATCCATGGTTTGCGACACGATAAC-3′ 
RSAPex11revsali 5′-ACGCGTCGAC TCATAGCACAGAAGACTCGGTC-3′ 
RSAPex25fwbamhi 5′-CGCGGATCCATGTCGTTTAACGACGATCT-3′ 
RSAPex25revsali 5′-ACGCGTCGAC­TCAATTCAGGC­AGGGATTTAGC-3′ 
RSARho1fwbamhi 5′-CGCGGATCCA­TGGCCGGACTA­GCAGAGATCAGG-3′ 
RSARho1revsali 5′-ACGCGTCGAC­TCACAAAATGA­CACACTTCTTC­TTTC-3′ 
EMK5 5′-CTTGAGGGAACTTTCACCATT-3′ 
EMK6 5′-ACGTGCACCACCCATTTCAG-3′ 
EMK11 5′-TCCCCGCGGC­TGTGCTCGTAG­ACCCAATTAAC-3′ 
EMK12 5′-CGGGATCCGT­ACGTTCCAGGA­AGAGAGTGAG-3′ 
RSARho1Not fw 5′-GCGGCCGCAT­TCTTATGGCCA­AGAAGACTACG­ATCGTC-3′ 
RSARho1HindIIIrev 5′-CCCAAGCTTGCGGCTGTGCTCGTAGACCC-3′ 
GFP-Rho1 fw 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTCTG­CCGGACTAGCA­GAGATCAG-3′ 
GFP-Rho1rev 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTCTC­ACAAAATGACA­CACTTCT-3′ 
Primer Name Sequence 
RSAPex25-1 5′-GGGGACAACT­TTGTATAGAAA­AGTTGCAAAGT­CTGGATGGAGG­CTTCATCTC-3′ 
RSAPex25-2 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGAGCGTG­GCATGCGGTTC­ATAGAAAC-3′ 
RSAPex25-3 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGGAGTCT­CTGCTCGCGTA­CAAGATC-3′ 
RSAPex25-4 5′-GGGGACAACT­TTGTATAATAA­AGTTGACTTGG­AGCTGCTGTGC­TTGTATG-3′ 
attB1-Ptef1-forward 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTGAT­CCCCCACACAC­CATAGCTTC-3′ 
attB2-Ttef1-reverse 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTGCT­CGTTTTCGACA­CTGGATGG-3′ 
RSAPex25-5 5′-CTGGATGGAGGCTTCATCTC-3′ 
RSAPex25-6 5′-GGAGCTGCTGTGCTTGTATG-3′ 
RSAPex11C-1 5′-GGGGACAACT­TTGTATAGAAA­AGTTGTACCAG­AGCTCATGTGC­TGTTCCAG-3′ 
RSAPex11C-2 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGAAGATC­CATAACAGACG­GTCGACAG-3′ 
RSAPex11C-3 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGCAACTG­GACGCACCTTG­AAAAGTC-3′ 
RSAPex11C-4 5′-GGGGACAACT­TTGTATAATAA­AGTTGGAAAGC­CGGTCTATCAG­GTCAAGC-3′ 
RSAPex11C-5 5′-ACCAGAGCTCATGTGCTGTTCCAG-3′ 
RSAPex11C-6 5′-GAAAGCCGGTCTATCAGGTCAAGC-3′ 
RSAPex11Cfusfw 5′-CCCAAGCTTT­GCTGCGACTGC­TAGCCAATCCCA-3′ 
RSAPex11Cfusrev 5′-AGATCTTCCA­ACAAGCTGGCG­CAACTGTGCAGA-3′ 
BB-JK-037 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTGTA­TGTCGTTTAAC­GACGATCTTTA­TAGGG-3′ 
BB-JK-038 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTTAT­TCAGGCAGGGA­TTTAGCTCCTT­TTCCG-3′ 
RSAatt PAOX F 5′-GGGGACAACT­TTGTATAGAAA­AGTTGGATCTC­GACGCGGAGAA­CGATC-3′ 
RSAattBIPrev 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGGAAACT­GCTGTGTTGTT­AGTG-3′ 
RSAattB1Pex3fwA 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTTGT­TCCAATATTGT­AGAGATCTT-3′ 
RSAattB2Pex3rev 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTAAG­CATCGAAATTA­GAGTAGAC-3′ 
RSA10fw 5′-GAAGATCTATGGTGAGCAAGGGCGAGGAG-3′ 
RSA11rev 5′-GCGTGTCGAC­TTACTTGTACA­GCTCGTCCATGCC-3′ 
RSA12Fw 5′-GGGGACAGCT­TTCTTGTACAA­AGTGGCCATGG­TGAGCAAGGGC­GAGGAG-3′ 
RSA13Rev 5′-GGGGACAACT­TTGTATAATAA­AGTTGCGATCT­GAACCTCGACT­TTCTG-3′ 
att PAOX F 5′-GGGGACAACT­TTGTATAGAAA­AGTTGGATCTC­GACGCGGAGAA­CGATC-3′ 
att PAOX R 5′-GGGGACTGCT­TTTTTGTACAA­ACTTGGTTTTT­GTACTTTAGAT­TGATGTCACC-3′ 
KN18 5′-CCCAAGCTTG­GATCCATGTTA­ACTTTCAATAA­GTC-3′ 
KN19 5′-GGGAAGCTTA­GATCTAAACTG­CTGTGTTGTTA­GTGGG-3′ 
KN14 5′-CCCCTCGAGA­ACCTGTACTTC­CAGTCGAGATC­TGTGAGCAAGG­GCGAGGAGC-3′ 
KN17 5′-GGGGTCGACT­TACAGCTCGTC­GTGAAGCTTGT­ACAGCTCG-3′ 
RSAPex11fwbamhi 5′-CGCGGATCCATGGTTTGCGACACGATAAC-3′ 
RSAPex11revsali 5′-ACGCGTCGAC TCATAGCACAGAAGACTCGGTC-3′ 
RSAPex25fwbamhi 5′-CGCGGATCCATGTCGTTTAACGACGATCT-3′ 
RSAPex25revsali 5′-ACGCGTCGAC­TCAATTCAGGC­AGGGATTTAGC-3′ 
RSARho1fwbamhi 5′-CGCGGATCCA­TGGCCGGACTA­GCAGAGATCAGG-3′ 
RSARho1revsali 5′-ACGCGTCGAC­TCACAAAATGA­CACACTTCTTC­TTTC-3′ 
EMK5 5′-CTTGAGGGAACTTTCACCATT-3′ 
EMK6 5′-ACGTGCACCACCCATTTCAG-3′ 
EMK11 5′-TCCCCGCGGC­TGTGCTCGTAG­ACCCAATTAAC-3′ 
EMK12 5′-CGGGATCCGT­ACGTTCCAGGA­AGAGAGTGAG-3′ 
RSARho1Not fw 5′-GCGGCCGCAT­TCTTATGGCCA­AGAAGACTACG­ATCGTC-3′ 
RSARho1HindIIIrev 5′-CCCAAGCTTGCGGCTGTGCTCGTAGACCC-3′ 
GFP-Rho1 fw 5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTCTG­CCGGACTAGCA­GAGATCAG-3′ 
GFP-Rho1rev 5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTCTC­ACAAAATGACA­CACTTCT-3′ 
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