Table III.

Plasmids used in this study

Plasmid Description Source/Reference 
pBluescript II Standard vector Fermentas 
pHIPZ4-DsRed-T1-SKL Plasmid containing PAOXDsRed-SKL, zeoR, ampR Monastyrska et al., 2005  
pANL29 pHIPZ4 containing PAOXGFP-SKL, zeoR, ampR Leao-Helder et al., 2003  
pHIPX4-GFP-SKL Plasmid containing PAOXGFP-SKL, zeoR, kanR Faber et al., 2002b  
pANL31 pHIPZ-eGFP fusionator, ampR Leao-Helder et al., 2003  
pSNA10 mGFP in pHIPZ vector, ampR Saraya et al., 2010  
pSNA03 Plasmid containing PAOXDsRed-SKL Nagotu et al., 2008b  
pCDNA3.1mCherry Plasmid containing mCherry, ampR Shaner et al., 2004  
pAG25 Plasmid containing nourseothricin marker, ampR Euroscarf 
pAG32 Plasmid containing hygromycine B marker, ampR Euroscarf 
pHIPZ4 pHIP containing zeocin marker, ampR Haan et al., 2002  
pHIPN4 pHIP containing nourseothricin marker, ampR This paper 
pHIPH4 pHIP containing hygromycine B marker, ampR This paper 
pHIPX7 pHIP containing leucine marker, kanR Baerends et al., 1996  
pHIPX4 Plasmid containing PAOX, Sc LEU2, kanR Gietl et al., 1994  
pENTR-PEX25 5′ pDONR-P4-P1R containing 5′ region of PEX25, kanR This paper 
pENTR-PEX25 3′ pDONR-P2R-P3 containing 3′ region of PEX25, kanR This paper 
pENTR-PEX11C 5′ pDONR-P4-P1R containing 5′ region of PEX11C, kanR This paper 
pENTR-PEX11C 3′ pDONR-P2R-P3 containing 3′ region of PEX11C, kanR This paper 
pENTR-221-NAT pENTR-221 containing nourseothricin marker, kanR This paper 
pENTR-221-HPH pENTR-221 containing hygromycine B marker, kanR This paper 
pAMK24 Plasmid containing PEX11C-GFP, ampR, zeoR This paper 
pMCE1 C-terminus of PEX25 fused toGFP in pSNA10, ampR Laboratory collection 
pHIPZ5-PEX3-GFP pHIPZ5 containing PEX3-GFP under control of amine oxidase promoter, zeoR Nagotu et al., 2008b  
pHOR46 Self-ligated 7.2-kb NotI-MluI (both Klenow-treated) fragment of pFEM152 Haan et al., 2002  
pDONR-P4-P1R Standard Gateway vector Invitrogen 
pDONR-P2R-P3 Standard Gateway vector Invitrogen 
pDONR-221 Standard Gateway vector Invitrogen 
pENTR-P4-P1R-PAOX pDONR-P4-P1R containing PAOX, kanR This paper 
pENTR-P4-P1R-PAOXBiPN30 pDONR-P4-P1R containing PAOXBIPN30, kanR This paper 
pENTR-221-PEX11 pENTR-221 containing PEX11, kanR This paper 
pENTR-221-PEX25 pENTR-221 containing PEX25, kanR This paper 
pENTR-221-PEX3-ATG pENTR-221 containing PEX3 without start codon, kanR This paper 
pDEST-R4-R3 Standard Gateway vector Invitrogen 
pDEST-R4-R3-NAT pDEST-R4-R3 containing nourseothricin marker, ampR This paper 
pRSA01 pHIPZ4-mCherry fusionator, zeoR This paper 
pRSA02 pDONR-P2R-P3 containing mCherry-TAMO, kanR This paper 
pRSA03 pDEST-R4-R3-NAT containing PEX11-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA06 pDEST-R4-R3-NAT containing PEX25-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA07 pDEST-R4-R3 containing zeocin marker, ampR This paper 
pRSA08 pRSA07 containing PEX25-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA017 pHIPZ4 containing BIPN30 fused to GFP-HDEL under control of alcohol oxidase promoter, zeoR, ampR This paper 
pRSA018 pDEST-R4-R3 containing PEX25 deletion cassette, nourseothricin marker, ampR This paper 
pRSA019 pDEST-R4-R3 containing PEX11C deletion cassette, hygromycine B marker, ampR This paper 
pRSA022 pRSA07 containing PEX11-mCherry under control of alcohol oxidase promoter, ampR This paper 
pEXP-BiPN30-Pex3-mCherry pRSA07 containing BIPN30PEX3-mCherry under control of alcohol oxidase promoter, ampR This paper 
pEXP-PEX11-GFP pDEST-R4-R3-NAT containing PEX11-GFP under control of alcohol oxidase promoter, ampR Nagotu et al., 2008b  
pREMI-Z REMI plasmid, ampR van Dijk et al., 2001  
pHIPZ4-Nia pHIPZ4 containing Nia, ampR Faber et al., 2002a  
pDEST-Zeo-tussen pDEST with Zeocin marker, ampR This paper 
pBS-BiP p-Bluescript II containing BIP This paper 
pBS-BiPN30-GFP-HDEL p-Bluescript II containing BIPN30-GFP-HDEL, ampR, This paper 
pHIPX7-BiPN30-GFP-HDEL pHIPX7 containing BIPN30 fused to GFP-HDEL, ScLEU2, kanR This paper 
pHIPX4-BiPN30-GFP-HDEL pHIPX4 containing BIPN30 fused to GFP-HDEL, ScLEU2, kanR This paper 
pBTM116-C Yeast two-hybrid vector containing LexA binding domain, ampR, TRP1 Takara Bio Inc. 
pVP16-C Yeast two-hybrid vector containing LexA activation domain, ampR, LEU2 Takara Bio Inc. 
pBTM116-PEX11 pBTM116 containing H. polymorpha PEX11 CDS, ampR, TRP1 This paper 
pVP16-PEX11 pVP16 containing H. polymorpha PEX11 CDS, ampR, LEU2 This paper 
pBTM116-PEX25 pBTM116 containing H. polymorpha PEX25 CDS, ampR, TRP1 This paper 
pVP16-PEX25 pVP16 containing H. polymorpha PEX25 CDS, ampR, LEU2 This paper 
pBTM116-RHO1 pBTM116 containing H. polymorpha RHO1CDS, ampR, TRP1 This paper 
pVP16-RHO1 pVP16 containing H. polymorpha RHO1 CDS, ampR, LEU2 This paper 
pBTM116-PEX3 pBTM116 containing H. polymorpha PEX3 CDS, ampR, TRP1 Saraya et al., 2010  
pVP16-PEX3 pVP16 containing H. polymorpha PEX3 CDS, ampR, LEU2 Saraya et al., 2010  
pBTM116-PEX19 pBTM116 containing H. polymorpha PEX19 CDS, ampR, TRP1 Saraya et al., 2010  
pVP16-PEX19 pVP16 containing H. polymorpha PEX19 CDS, ampR, LEU2 Saraya et al., 2010  
pR6-5 pBS URA3 containing RHO1ts This paper 
pBSK-URA3 pBluescript II containing H. polymorpha URA3 Leao-Helder et al., 2003  
pHIPH-Rho1 Plasmid containing RHO1ts and hygromycin marker This paper 
pENTR-221-RHO1 pENTR-221 containing RHO1, kanR This paper 
pENTR/41-PAMO-GFP Gateway vector containing PAMO GFP Nagotu et al., 2008a  
pENTR/23-TAMO Gateway vector containing AMO terminator Nagotu et al., 2008a  
pEXP-GFPRho1 pRSA07 containing GFP-RHO1 under control of the amine oxidase promoter, ampR This paper 
Plasmid Description Source/Reference 
pBluescript II Standard vector Fermentas 
pHIPZ4-DsRed-T1-SKL Plasmid containing PAOXDsRed-SKL, zeoR, ampR Monastyrska et al., 2005  
pANL29 pHIPZ4 containing PAOXGFP-SKL, zeoR, ampR Leao-Helder et al., 2003  
pHIPX4-GFP-SKL Plasmid containing PAOXGFP-SKL, zeoR, kanR Faber et al., 2002b  
pANL31 pHIPZ-eGFP fusionator, ampR Leao-Helder et al., 2003  
pSNA10 mGFP in pHIPZ vector, ampR Saraya et al., 2010  
pSNA03 Plasmid containing PAOXDsRed-SKL Nagotu et al., 2008b  
pCDNA3.1mCherry Plasmid containing mCherry, ampR Shaner et al., 2004  
pAG25 Plasmid containing nourseothricin marker, ampR Euroscarf 
pAG32 Plasmid containing hygromycine B marker, ampR Euroscarf 
pHIPZ4 pHIP containing zeocin marker, ampR Haan et al., 2002  
pHIPN4 pHIP containing nourseothricin marker, ampR This paper 
pHIPH4 pHIP containing hygromycine B marker, ampR This paper 
pHIPX7 pHIP containing leucine marker, kanR Baerends et al., 1996  
pHIPX4 Plasmid containing PAOX, Sc LEU2, kanR Gietl et al., 1994  
pENTR-PEX25 5′ pDONR-P4-P1R containing 5′ region of PEX25, kanR This paper 
pENTR-PEX25 3′ pDONR-P2R-P3 containing 3′ region of PEX25, kanR This paper 
pENTR-PEX11C 5′ pDONR-P4-P1R containing 5′ region of PEX11C, kanR This paper 
pENTR-PEX11C 3′ pDONR-P2R-P3 containing 3′ region of PEX11C, kanR This paper 
pENTR-221-NAT pENTR-221 containing nourseothricin marker, kanR This paper 
pENTR-221-HPH pENTR-221 containing hygromycine B marker, kanR This paper 
pAMK24 Plasmid containing PEX11C-GFP, ampR, zeoR This paper 
pMCE1 C-terminus of PEX25 fused toGFP in pSNA10, ampR Laboratory collection 
pHIPZ5-PEX3-GFP pHIPZ5 containing PEX3-GFP under control of amine oxidase promoter, zeoR Nagotu et al., 2008b  
pHOR46 Self-ligated 7.2-kb NotI-MluI (both Klenow-treated) fragment of pFEM152 Haan et al., 2002  
pDONR-P4-P1R Standard Gateway vector Invitrogen 
pDONR-P2R-P3 Standard Gateway vector Invitrogen 
pDONR-221 Standard Gateway vector Invitrogen 
pENTR-P4-P1R-PAOX pDONR-P4-P1R containing PAOX, kanR This paper 
pENTR-P4-P1R-PAOXBiPN30 pDONR-P4-P1R containing PAOXBIPN30, kanR This paper 
pENTR-221-PEX11 pENTR-221 containing PEX11, kanR This paper 
pENTR-221-PEX25 pENTR-221 containing PEX25, kanR This paper 
pENTR-221-PEX3-ATG pENTR-221 containing PEX3 without start codon, kanR This paper 
pDEST-R4-R3 Standard Gateway vector Invitrogen 
pDEST-R4-R3-NAT pDEST-R4-R3 containing nourseothricin marker, ampR This paper 
pRSA01 pHIPZ4-mCherry fusionator, zeoR This paper 
pRSA02 pDONR-P2R-P3 containing mCherry-TAMO, kanR This paper 
pRSA03 pDEST-R4-R3-NAT containing PEX11-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA06 pDEST-R4-R3-NAT containing PEX25-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA07 pDEST-R4-R3 containing zeocin marker, ampR This paper 
pRSA08 pRSA07 containing PEX25-mCherry under control of alcohol oxidase promoter, ampR This paper 
pRSA017 pHIPZ4 containing BIPN30 fused to GFP-HDEL under control of alcohol oxidase promoter, zeoR, ampR This paper 
pRSA018 pDEST-R4-R3 containing PEX25 deletion cassette, nourseothricin marker, ampR This paper 
pRSA019 pDEST-R4-R3 containing PEX11C deletion cassette, hygromycine B marker, ampR This paper 
pRSA022 pRSA07 containing PEX11-mCherry under control of alcohol oxidase promoter, ampR This paper 
pEXP-BiPN30-Pex3-mCherry pRSA07 containing BIPN30PEX3-mCherry under control of alcohol oxidase promoter, ampR This paper 
pEXP-PEX11-GFP pDEST-R4-R3-NAT containing PEX11-GFP under control of alcohol oxidase promoter, ampR Nagotu et al., 2008b  
pREMI-Z REMI plasmid, ampR van Dijk et al., 2001  
pHIPZ4-Nia pHIPZ4 containing Nia, ampR Faber et al., 2002a  
pDEST-Zeo-tussen pDEST with Zeocin marker, ampR This paper 
pBS-BiP p-Bluescript II containing BIP This paper 
pBS-BiPN30-GFP-HDEL p-Bluescript II containing BIPN30-GFP-HDEL, ampR, This paper 
pHIPX7-BiPN30-GFP-HDEL pHIPX7 containing BIPN30 fused to GFP-HDEL, ScLEU2, kanR This paper 
pHIPX4-BiPN30-GFP-HDEL pHIPX4 containing BIPN30 fused to GFP-HDEL, ScLEU2, kanR This paper 
pBTM116-C Yeast two-hybrid vector containing LexA binding domain, ampR, TRP1 Takara Bio Inc. 
pVP16-C Yeast two-hybrid vector containing LexA activation domain, ampR, LEU2 Takara Bio Inc. 
pBTM116-PEX11 pBTM116 containing H. polymorpha PEX11 CDS, ampR, TRP1 This paper 
pVP16-PEX11 pVP16 containing H. polymorpha PEX11 CDS, ampR, LEU2 This paper 
pBTM116-PEX25 pBTM116 containing H. polymorpha PEX25 CDS, ampR, TRP1 This paper 
pVP16-PEX25 pVP16 containing H. polymorpha PEX25 CDS, ampR, LEU2 This paper 
pBTM116-RHO1 pBTM116 containing H. polymorpha RHO1CDS, ampR, TRP1 This paper 
pVP16-RHO1 pVP16 containing H. polymorpha RHO1 CDS, ampR, LEU2 This paper 
pBTM116-PEX3 pBTM116 containing H. polymorpha PEX3 CDS, ampR, TRP1 Saraya et al., 2010  
pVP16-PEX3 pVP16 containing H. polymorpha PEX3 CDS, ampR, LEU2 Saraya et al., 2010  
pBTM116-PEX19 pBTM116 containing H. polymorpha PEX19 CDS, ampR, TRP1 Saraya et al., 2010  
pVP16-PEX19 pVP16 containing H. polymorpha PEX19 CDS, ampR, LEU2 Saraya et al., 2010  
pR6-5 pBS URA3 containing RHO1ts This paper 
pBSK-URA3 pBluescript II containing H. polymorpha URA3 Leao-Helder et al., 2003  
pHIPH-Rho1 Plasmid containing RHO1ts and hygromycin marker This paper 
pENTR-221-RHO1 pENTR-221 containing RHO1, kanR This paper 
pENTR/41-PAMO-GFP Gateway vector containing PAMO GFP Nagotu et al., 2008a  
pENTR/23-TAMO Gateway vector containing AMO terminator Nagotu et al., 2008a  
pEXP-GFPRho1 pRSA07 containing GFP-RHO1 under control of the amine oxidase promoter, ampR This paper 
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