Table II.

Yeast strains used in this study

Strain Description 
WT NCYC495 ura3 leu1.1 (Sudbery et al., 1988) 
WT DsRed-T1-SKL WT with pHIPZ4-DsRed-T1-SKL, leu1.1 (Monastyrska et al., 2005) 
pex11 PEX11 deletion, leu1.1 (Krikken et al., 2009) 
pex11.GFP-SKL pex11 with pHIPZ4-GFP-SKL (Nagotu et al., 2008b) 
pex25 PEX25 deletion, leu1.1, ura3 
pex25.DsRed-SKL pex25 with pHIPZ4-DsRed-T1-SKL 
WT.Pex11-GFP WT with pEXP-PEX11-GFP 
WT.Pex25-GFP WT with pMCE1 
WT.Pex11C-GFP WT with pAMK24 
pex11C PEX11C deletion, leu1.1, ura3 
pex11C. DsRed-SKL pex11C with pHIPZ4-DsRed-T1-SKL 
pex11 pex25 PEX11 PEX25 double deletion strain 
pex11 pex25.DsRed-SKL pex11 pex25 with pHIPZ4-DsRed-T1-SKL 
pex11 pex25.Pex3-GFP pex11 pex25 with pHOR46 
pex11 pex25.Pex11-mCherry pex11 pex25 with pRSA022 
pex11 pex25.Pex11-mCherry Pex3-GFP pex11 pex25 with pRSA022 and pHOR46 
pex11 pex25.Pex11-mCherry.BiPN30-GFP-HDEL pex11 pex25 with pRSA022 and pHIPX4-BiPN30-GFP-HDEL 
pex11 pex25.Pex11-mCherry.GFP-SKL pex11 pex25 with pRSA022 and pHIPX4-GFP-SKL 
pex11 pex25.Pex25-mCherry pex11 pex25 with pRSA08 
pex11 pex25.Pex25-mCherry Pex3-GFP pex11 pex25 with pRSA08 and pHOR46 
pex11 pex25.Pex25-mCherry.BiPN30-GFP-HDEL pex11 pex25 with pRSA08 and pHIPX4-BiPN30-GFP-HDEL 
pex11 pex25.Pex25-mCherry.GFP-SKL pex11 pex25 with pRSA08 and pHIPX4-GFP-SKL 
pex11 pex11C PEX11 PEX11C double deletion strain 
pex11 pex11C.DsRed-SKL pex11 pex11C with pHIPZ4-DsRed-T1-SKL 
pex25 pex11C PEX25 PEX11C double deletion strain 
pex25 pex11C.DsRed-SKL pex25 pex11C with pHIPZ4-T1-DsRed-SKL 
pex11.Pex11-mCherry pex11 with pRSA03 
pex3 pex11 PEX3 PEX11 double deletion strain 
pex3 pex11.Pex3-GFP pex3 pex11 with pHIPZ5-Pex3-GFP 
pex3 pex11.Pex11-mCherry pex3 pex11 with pRSA03 
pex3 pex11.Pex11-mCherry.BiPN30-GFP-HDEL pex3 pex11.Pex11-mCherry with pRSA017 
pex3.Pex25-mCherry.BiPN30-GFP-HDEL pex3 deletion strain with pRSA06 and pRSA017 
pex3.Pex3-GFP PEX3 deletion with pHIPZ5-Pex3-GFP (Nagotu et al., 2008b) 
pex3 PEX3 deletion strain, ura3 (Baerends et al., 1996) 
pex3 pex25 PEX3 PEX25 double deletion strain 
pex3 pex25.Pex3-GFP pex3 pex25 with pHIPZ5-Pex3-GFP 
pex3 pex11C PEX3 PEX11C double deletion strain 
pex3 pex11C.Pex3-GFP pex3 pex11C with pHIPZ5-Pex3-GFP 
pex3 pex11 pex25 PEX3 PEX11 PEX25 triple deletion strain 
pex3. BiPN30Pex3-mCherry PEX3 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex11.BiPN30Pex3-mCherry PEX3 PEX11 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex25.BiPN30Pex3-mCherry PEX3 PEX25 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex11 pex25.BiPN30Pex3-mCherry PEX3 PEX11 PEX25 deletion with pEXP-BiPN30-PEX3-mCherry 
L-40 S. cerevisiae MATa leu2 his3 trp1 ade2 GAL4 gal80 LYS2::(lexAop)4-HIS3 URA3::(lexAop)s-lacZ (Takara Bio Inc.) 
WT.GFP-Rho1 WT with pEXP-GFPRho1 
WT.GFP-Rho1 DsRed-SKL WT with pEXP-GFPRho1 and pSNA03 
pex11 pex25 RHO1tsPex25-mCherry PEX11 PEX25 double deletion with pHIPH-Rho1 and pRSA08 
pex3 N50.Pex3-GFP PEX3 deletion with N50.Pex3-GFP (Faber et al., 2002a) 
pex3 RHO1tsN50.Pex3-GFP PEX3 deletion with N50.Pex3-GFP with RHO1ts 
Strain Description 
WT NCYC495 ura3 leu1.1 (Sudbery et al., 1988) 
WT DsRed-T1-SKL WT with pHIPZ4-DsRed-T1-SKL, leu1.1 (Monastyrska et al., 2005) 
pex11 PEX11 deletion, leu1.1 (Krikken et al., 2009) 
pex11.GFP-SKL pex11 with pHIPZ4-GFP-SKL (Nagotu et al., 2008b) 
pex25 PEX25 deletion, leu1.1, ura3 
pex25.DsRed-SKL pex25 with pHIPZ4-DsRed-T1-SKL 
WT.Pex11-GFP WT with pEXP-PEX11-GFP 
WT.Pex25-GFP WT with pMCE1 
WT.Pex11C-GFP WT with pAMK24 
pex11C PEX11C deletion, leu1.1, ura3 
pex11C. DsRed-SKL pex11C with pHIPZ4-DsRed-T1-SKL 
pex11 pex25 PEX11 PEX25 double deletion strain 
pex11 pex25.DsRed-SKL pex11 pex25 with pHIPZ4-DsRed-T1-SKL 
pex11 pex25.Pex3-GFP pex11 pex25 with pHOR46 
pex11 pex25.Pex11-mCherry pex11 pex25 with pRSA022 
pex11 pex25.Pex11-mCherry Pex3-GFP pex11 pex25 with pRSA022 and pHOR46 
pex11 pex25.Pex11-mCherry.BiPN30-GFP-HDEL pex11 pex25 with pRSA022 and pHIPX4-BiPN30-GFP-HDEL 
pex11 pex25.Pex11-mCherry.GFP-SKL pex11 pex25 with pRSA022 and pHIPX4-GFP-SKL 
pex11 pex25.Pex25-mCherry pex11 pex25 with pRSA08 
pex11 pex25.Pex25-mCherry Pex3-GFP pex11 pex25 with pRSA08 and pHOR46 
pex11 pex25.Pex25-mCherry.BiPN30-GFP-HDEL pex11 pex25 with pRSA08 and pHIPX4-BiPN30-GFP-HDEL 
pex11 pex25.Pex25-mCherry.GFP-SKL pex11 pex25 with pRSA08 and pHIPX4-GFP-SKL 
pex11 pex11C PEX11 PEX11C double deletion strain 
pex11 pex11C.DsRed-SKL pex11 pex11C with pHIPZ4-DsRed-T1-SKL 
pex25 pex11C PEX25 PEX11C double deletion strain 
pex25 pex11C.DsRed-SKL pex25 pex11C with pHIPZ4-T1-DsRed-SKL 
pex11.Pex11-mCherry pex11 with pRSA03 
pex3 pex11 PEX3 PEX11 double deletion strain 
pex3 pex11.Pex3-GFP pex3 pex11 with pHIPZ5-Pex3-GFP 
pex3 pex11.Pex11-mCherry pex3 pex11 with pRSA03 
pex3 pex11.Pex11-mCherry.BiPN30-GFP-HDEL pex3 pex11.Pex11-mCherry with pRSA017 
pex3.Pex25-mCherry.BiPN30-GFP-HDEL pex3 deletion strain with pRSA06 and pRSA017 
pex3.Pex3-GFP PEX3 deletion with pHIPZ5-Pex3-GFP (Nagotu et al., 2008b) 
pex3 PEX3 deletion strain, ura3 (Baerends et al., 1996) 
pex3 pex25 PEX3 PEX25 double deletion strain 
pex3 pex25.Pex3-GFP pex3 pex25 with pHIPZ5-Pex3-GFP 
pex3 pex11C PEX3 PEX11C double deletion strain 
pex3 pex11C.Pex3-GFP pex3 pex11C with pHIPZ5-Pex3-GFP 
pex3 pex11 pex25 PEX3 PEX11 PEX25 triple deletion strain 
pex3. BiPN30Pex3-mCherry PEX3 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex11.BiPN30Pex3-mCherry PEX3 PEX11 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex25.BiPN30Pex3-mCherry PEX3 PEX25 deletion with pEXP-BiPN30-PEX3-mCherry 
pex3 pex11 pex25.BiPN30Pex3-mCherry PEX3 PEX11 PEX25 deletion with pEXP-BiPN30-PEX3-mCherry 
L-40 S. cerevisiae MATa leu2 his3 trp1 ade2 GAL4 gal80 LYS2::(lexAop)4-HIS3 URA3::(lexAop)s-lacZ (Takara Bio Inc.) 
WT.GFP-Rho1 WT with pEXP-GFPRho1 
WT.GFP-Rho1 DsRed-SKL WT with pEXP-GFPRho1 and pSNA03 
pex11 pex25 RHO1tsPex25-mCherry PEX11 PEX25 double deletion with pHIPH-Rho1 and pRSA08 
pex3 N50.Pex3-GFP PEX3 deletion with N50.Pex3-GFP (Faber et al., 2002a) 
pex3 RHO1tsN50.Pex3-GFP PEX3 deletion with N50.Pex3-GFP with RHO1ts 
View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal