Ig gene usage, CDR3 amino acid features, and reactivity of recombinant citrulline-specific IgG antibodies generated from synovial memory B cells of RA patients
| Clone | Heavy-chain | Light-chain | Reactivity | ||||||||||||||||||
| V | D | J | IgG SC | V-Mut. | CDR3 (aa) | (+) | Length | Family | V | J | V-Mut. | CDR3 (aa) | (+) | Length | CEP-1 | cit-fib | cit-vim | CCP | Polyreactivity | Tetanus | |
| 1276SF-B01 | 3-49 | 3-3 | 3 | IgG1 | 4 (3R/1S) | EYVDFWSDPSRARFDI | 2 | 16 | λ | 1-44 | 3 | 2 (2R) | AAWDDSLNGWV | 0 | 11 | + | + | + | neg | neg | neg |
| 1276SF-B07 | 3-23 | 4-4 | 4 | IgG1 | 8 (7R/1S) | DWRHNNYGPPHSFDY | 3 | 15 | λ | 2-14 | 3 | 4 (3R/1S) | SSYTSSSTWV | 0 | 10 | − | + | − | neg | neg | neg |
| 1276SF-C07 | 3-7 | 3-10 | 4 | IgG1 | 6 (4R/2S) | RGKCFFDC | 2 | 8 | λ | 7-43 | 2 | 6 (3R/3S) | VLYMGSGISV | 0 | 10 | ++ | − | + | pos | neg | neg |
| 1276SF-D10 | 3-30 | 6-13 | 6 | IgG1 | 6 (4R/2S) | VRGAAATGYYYGMDV | 1 | 15 | λ | 3-1 | 2 | 6 (6R) | QAWDSSTVV | 0 | 9 | +++ | ++ | ++ | pos | pos | neg |
| 1276SF-F12 | 4-59 | 4-17 | 4 | IgG1 | 3 (2R/1S) | RLLGDYIFDY | 1 | 10 | λ | 3-1 | 2 | 4 (4R) | VRRGTAAVV | 2 | 9 | − | + | + | pos | pos | neg |
| 1276SF-G08 | 4-39 | 3-10 | 4 | IgG1 | 1 (1R) | VRGYFDY | 1 | 7 | κ | 3-15 | 1 | 3 (2R/1S) | QHYNNWPPWT | 1 | 10 | ++ | + | + | neg | neg | neg |
| 1276SF-H10 | 4-39 | 3-22 | 4 | IgG1 | 4 (3R/1S) | LPNYYDSSGYARGGFDY | 1 | 17 | κ | 3-15 | 3 | 1 (1R) | QQYNNWPRGGFT | 1 | 12 | + | − | − | pos | neg | neg |
| 1103SF-A03 | 4-39 | 5-18 | 4 | IgG1 | 7 (2R/5S) | RRGYSYGYSRARGTTFDY | 5 | 20 | λ | 1-51 | 3 | 9 (6R/3S) | GTWDSSLSAGV | 0 | 11 | +++ | ++ | − | neg | neg | neg |
| 1103SF-A04 | 1-2 | 3-3 | 3 | IgG1 | 16 (14R/2S) | DRSPIDYDFWSGSTFYSYGMDV | 2 | 24 | λ | 1-47 | 2 | 6 (4R/2S) | AAWDDSLSGVV | 0 | 11 | +++ | + | ++ | pos | pos | neg |
| 1103SF-B02 | 1-69 | 3-9 | 4 | IgG1 | 8 (5R/3S) | INTSPILTGYYFPGVHDY | 1 | 18 | κ | 1-12 | 3 | 6 (4R/2S) | QQANSFPFT | 0 | 9 | ++ | + | + | pos | pos | neg |
| 1103SF-B03 | 4-31 | 3-22 | 4 | IgG1 | 3 (2R/1S) | GAREGLIVVDTFDY | 1 | 14 | κ | 1-39 | 3 | 4 (4R) | HQSYSTPQT | 1 | 9 | + | − | − | neg | neg | neg |
| 1103SF-B04 | 3-30-3 | 2-2 | 5 | IgG1 | 4 (3R/1S) | DLGDTSCYT | 0 | 10 | κ | 1-39 | 1 | 5 (4R/1S) | QQSYSTPQT | 0 | 9 | +++ | − | − | neg | neg | neg |
| 1103SF-H05 | 3-30-3 | 6-6 | 4 | IgG1 | 2 (1R/1S) | DPYRGKATQDY | 2 | 11 | κ | 1-39 | 3 | 7 (5R/2S) | QQSYSTPFT | 0 | 9 | + | + | − | pos | pos | neg |
| 1325SF-A04 | 3-23 | 2-21 | 4 | IgG1 | 34 (27R/7S) | VKAWEIIAS | 1 | 9 | λ | 3-21 | 3 | 29 (21R/8S) | QVWDSSADHPV | 1 | 11 | +++ | + | − | pos | neg | neg |
| 1325SF-B05 | 1-8 | 4-23 | 6 | IgG1 | 25 (18R/7S) | GGRPYYYYYGMDV | 2 | 17 | λ | 2-8 | 2 | 26 (19R/7S) | SSYAGGNVVV | 0 | 10 | ++ | − | − | pos | neg | neg |
| 1325SF-B07 | 3-9 | 3-22 | 4 | IgG1 | 2 (1R/1S) | GARASDSSGSNYFDY | 1 | 15 | κ | 1-5 | 5 | 4 (3R/1S) | QQHNHYSPIT | 2 | 10 | + | − | − | neg | neg | neg |
| 1325SF-C02 | 1-2 | 3-10 | 4 | IgG1 | 2 (1R/1S) | SGASITMIRGALEN | 1 | 14 | λ | 1-47 | 3 | 7 (5R/2S) | AAWDDSLRWV | 1 | 10 | + | − | − | neg | neg | neg |
| 1325SF-C04 | 3-21 | 4-23 | 4 | IgG1 | 8 (3R/5S) | GITVITPGSY | 0 | 11 | λ | 3-21 | 2 | 10 (7R/3S) | QVWDTSSDHHVV | 2 | 12 | ++ | − | − | pos | pos | neg |
| 1325SF-C05 | 1-2 | 4-17 | 5 | IgG1 | 8 (8R) | VMDPRPPYGDYAISH | 2 | 15 | λ | 1-47 | 2 | 9 (8R/1S) | AAWDDSLR | 1 | 8 | ++ | − | − | pos | neg | neg |
| 1325SF-D06 | 3-74 | 5-24 | 2 | IgG1 | 25 (15R/10S) | DAGNSGHDWYFDL | 1 | 13 | λ | 2-23 | 3 | 19 (15R/4S) | CSYAGRGLGV | 1 | 10 | +++ | +++ | + | pos | neg | neg |
| 1325SF-G12 | 4-39 | 5-24 | 4 | IgG3 | 5 (4R/1S) | HRNPPIAIVIFVFSPMDY | 1 | 18 | λ | 1-51 | 1 | 11 (7R/4S) | GTWDTSLNVPYV | 0 | 12 | + | − | − | pos | neg | neg |
| 1325SF-H07 | 3-23 | 3-3 | 4 | IgG1 | 3 (2R/1S) | GSLYDFWSGYPDSFDY | 0 | 16 | κ | 1-5 | 1 | 8 (6R/2S) | QQYNTYLWT | 0 | 9 | + | − | + | pos | pos | neg |
| 1325SF-B109 | 3-49 | 3-3 | 3 | IgG1 | 4 (4R) | TRDEYYDFWSGPSRAFDI | 2 | 18 | λ | 1-44 | 3 | 2 (2R) | AAWDDSLNGWV | 0 | 11 | ++ | + | + | neg | neg | neg |
| 1325SF-B117 | 4-59 | 5-12 | 4 | IgG2 | 13 (6R/7S) | LDVEYSGFDLAYYFDS | 1 | 18 | λ | 2-23 | 3 | 19 (16R/3S) | CSHARSYSLV | 2 | 10 | +++ | − | − | neg | neg | neg |
| 1325SF-C127 | 3-15 | 2-15 | 6 | IgG1 | 5 (4R/1S) | TTDPGYCSGGRCYHHFYYGMDV | 3 | 22 | λ | 1-51 | 3 | 5 (2R/3S) | GTWDSSLSPWV | 0 | 11 | ++ | ++ | − | neg | pos | neg |
| Clone | Heavy-chain | Light-chain | Reactivity | ||||||||||||||||||
| V | D | J | IgG SC | V-Mut. | CDR3 (aa) | (+) | Length | Family | V | J | V-Mut. | CDR3 (aa) | (+) | Length | CEP-1 | cit-fib | cit-vim | CCP | Polyreactivity | Tetanus | |
| 1276SF-B01 | 3-49 | 3-3 | 3 | IgG1 | 4 (3R/1S) | EYVDFWSDPSRARFDI | 2 | 16 | λ | 1-44 | 3 | 2 (2R) | AAWDDSLNGWV | 0 | 11 | + | + | + | neg | neg | neg |
| 1276SF-B07 | 3-23 | 4-4 | 4 | IgG1 | 8 (7R/1S) | DWRHNNYGPPHSFDY | 3 | 15 | λ | 2-14 | 3 | 4 (3R/1S) | SSYTSSSTWV | 0 | 10 | − | + | − | neg | neg | neg |
| 1276SF-C07 | 3-7 | 3-10 | 4 | IgG1 | 6 (4R/2S) | RGKCFFDC | 2 | 8 | λ | 7-43 | 2 | 6 (3R/3S) | VLYMGSGISV | 0 | 10 | ++ | − | + | pos | neg | neg |
| 1276SF-D10 | 3-30 | 6-13 | 6 | IgG1 | 6 (4R/2S) | VRGAAATGYYYGMDV | 1 | 15 | λ | 3-1 | 2 | 6 (6R) | QAWDSSTVV | 0 | 9 | +++ | ++ | ++ | pos | pos | neg |
| 1276SF-F12 | 4-59 | 4-17 | 4 | IgG1 | 3 (2R/1S) | RLLGDYIFDY | 1 | 10 | λ | 3-1 | 2 | 4 (4R) | VRRGTAAVV | 2 | 9 | − | + | + | pos | pos | neg |
| 1276SF-G08 | 4-39 | 3-10 | 4 | IgG1 | 1 (1R) | VRGYFDY | 1 | 7 | κ | 3-15 | 1 | 3 (2R/1S) | QHYNNWPPWT | 1 | 10 | ++ | + | + | neg | neg | neg |
| 1276SF-H10 | 4-39 | 3-22 | 4 | IgG1 | 4 (3R/1S) | LPNYYDSSGYARGGFDY | 1 | 17 | κ | 3-15 | 3 | 1 (1R) | QQYNNWPRGGFT | 1 | 12 | + | − | − | pos | neg | neg |
| 1103SF-A03 | 4-39 | 5-18 | 4 | IgG1 | 7 (2R/5S) | RRGYSYGYSRARGTTFDY | 5 | 20 | λ | 1-51 | 3 | 9 (6R/3S) | GTWDSSLSAGV | 0 | 11 | +++ | ++ | − | neg | neg | neg |
| 1103SF-A04 | 1-2 | 3-3 | 3 | IgG1 | 16 (14R/2S) | DRSPIDYDFWSGSTFYSYGMDV | 2 | 24 | λ | 1-47 | 2 | 6 (4R/2S) | AAWDDSLSGVV | 0 | 11 | +++ | + | ++ | pos | pos | neg |
| 1103SF-B02 | 1-69 | 3-9 | 4 | IgG1 | 8 (5R/3S) | INTSPILTGYYFPGVHDY | 1 | 18 | κ | 1-12 | 3 | 6 (4R/2S) | QQANSFPFT | 0 | 9 | ++ | + | + | pos | pos | neg |
| 1103SF-B03 | 4-31 | 3-22 | 4 | IgG1 | 3 (2R/1S) | GAREGLIVVDTFDY | 1 | 14 | κ | 1-39 | 3 | 4 (4R) | HQSYSTPQT | 1 | 9 | + | − | − | neg | neg | neg |
| 1103SF-B04 | 3-30-3 | 2-2 | 5 | IgG1 | 4 (3R/1S) | DLGDTSCYT | 0 | 10 | κ | 1-39 | 1 | 5 (4R/1S) | QQSYSTPQT | 0 | 9 | +++ | − | − | neg | neg | neg |
| 1103SF-H05 | 3-30-3 | 6-6 | 4 | IgG1 | 2 (1R/1S) | DPYRGKATQDY | 2 | 11 | κ | 1-39 | 3 | 7 (5R/2S) | QQSYSTPFT | 0 | 9 | + | + | − | pos | pos | neg |
| 1325SF-A04 | 3-23 | 2-21 | 4 | IgG1 | 34 (27R/7S) | VKAWEIIAS | 1 | 9 | λ | 3-21 | 3 | 29 (21R/8S) | QVWDSSADHPV | 1 | 11 | +++ | + | − | pos | neg | neg |
| 1325SF-B05 | 1-8 | 4-23 | 6 | IgG1 | 25 (18R/7S) | GGRPYYYYYGMDV | 2 | 17 | λ | 2-8 | 2 | 26 (19R/7S) | SSYAGGNVVV | 0 | 10 | ++ | − | − | pos | neg | neg |
| 1325SF-B07 | 3-9 | 3-22 | 4 | IgG1 | 2 (1R/1S) | GARASDSSGSNYFDY | 1 | 15 | κ | 1-5 | 5 | 4 (3R/1S) | QQHNHYSPIT | 2 | 10 | + | − | − | neg | neg | neg |
| 1325SF-C02 | 1-2 | 3-10 | 4 | IgG1 | 2 (1R/1S) | SGASITMIRGALEN | 1 | 14 | λ | 1-47 | 3 | 7 (5R/2S) | AAWDDSLRWV | 1 | 10 | + | − | − | neg | neg | neg |
| 1325SF-C04 | 3-21 | 4-23 | 4 | IgG1 | 8 (3R/5S) | GITVITPGSY | 0 | 11 | λ | 3-21 | 2 | 10 (7R/3S) | QVWDTSSDHHVV | 2 | 12 | ++ | − | − | pos | pos | neg |
| 1325SF-C05 | 1-2 | 4-17 | 5 | IgG1 | 8 (8R) | VMDPRPPYGDYAISH | 2 | 15 | λ | 1-47 | 2 | 9 (8R/1S) | AAWDDSLR | 1 | 8 | ++ | − | − | pos | neg | neg |
| 1325SF-D06 | 3-74 | 5-24 | 2 | IgG1 | 25 (15R/10S) | DAGNSGHDWYFDL | 1 | 13 | λ | 2-23 | 3 | 19 (15R/4S) | CSYAGRGLGV | 1 | 10 | +++ | +++ | + | pos | neg | neg |
| 1325SF-G12 | 4-39 | 5-24 | 4 | IgG3 | 5 (4R/1S) | HRNPPIAIVIFVFSPMDY | 1 | 18 | λ | 1-51 | 1 | 11 (7R/4S) | GTWDTSLNVPYV | 0 | 12 | + | − | − | pos | neg | neg |
| 1325SF-H07 | 3-23 | 3-3 | 4 | IgG1 | 3 (2R/1S) | GSLYDFWSGYPDSFDY | 0 | 16 | κ | 1-5 | 1 | 8 (6R/2S) | QQYNTYLWT | 0 | 9 | + | − | + | pos | pos | neg |
| 1325SF-B109 | 3-49 | 3-3 | 3 | IgG1 | 4 (4R) | TRDEYYDFWSGPSRAFDI | 2 | 18 | λ | 1-44 | 3 | 2 (2R) | AAWDDSLNGWV | 0 | 11 | ++ | + | + | neg | neg | neg |
| 1325SF-B117 | 4-59 | 5-12 | 4 | IgG2 | 13 (6R/7S) | LDVEYSGFDLAYYFDS | 1 | 18 | λ | 2-23 | 3 | 19 (16R/3S) | CSHARSYSLV | 2 | 10 | +++ | − | − | neg | neg | neg |
| 1325SF-C127 | 3-15 | 2-15 | 6 | IgG1 | 5 (4R/1S) | TTDPGYCSGGRCYHHFYYGMDV | 3 | 22 | λ | 1-51 | 3 | 5 (2R/3S) | GTWDSSLSPWV | 0 | 11 | ++ | ++ | − | neg | pos | neg |
For reactivity, increasing signal strength is denoted as + (negative control signal < OD 450 nm < 1.0), ++ (1.0 < OD 450 nm < 2.0), and +++ (OD 450 nm > 2.0).