List of primary antibodies used
| Name . | Type . | Dilution . | Company . |
|---|---|---|---|
| MAP2 | Polyclonal | 1:500 (ICC) | 5392; Abcam |
| VGLUT1 | Polyclonal | 1:1,000 (ICC) | 135302; SySy |
| HA | Monoclonal | 1:500 (ICC) | 12CA5; Roche |
| FLAG | Monoclonal | 1:1,000 (ICC) | F1804; Sigma-Aldrich |
| RIM1 | Polyclonal | 1:500 (ICC), 1:1,000 (WB) | 140003; SySy |
| MUNC13-1 | Polyclonal | 1:1,000 (ICC, WB) | 126103; SySy |
| α-tubulin | Monoclonal | 1:10,000 (WB) | 302211; SySy |
| p62/SQSTM1 | Polyclonal | 1:2,000 (WB) | PA5–20839; Thermo Fisher Scientific |
| Name . | Type . | Dilution . | Company . |
|---|---|---|---|
| MAP2 | Polyclonal | 1:500 (ICC) | 5392; Abcam |
| VGLUT1 | Polyclonal | 1:1,000 (ICC) | 135302; SySy |
| HA | Monoclonal | 1:500 (ICC) | 12CA5; Roche |
| FLAG | Monoclonal | 1:1,000 (ICC) | F1804; Sigma-Aldrich |
| RIM1 | Polyclonal | 1:500 (ICC), 1:1,000 (WB) | 140003; SySy |
| MUNC13-1 | Polyclonal | 1:1,000 (ICC, WB) | 126103; SySy |
| α-tubulin | Monoclonal | 1:10,000 (WB) | 302211; SySy |
| p62/SQSTM1 | Polyclonal | 1:2,000 (WB) | PA5–20839; Thermo Fisher Scientific |