The difference between the melting temperatures, ΔTm, of αTM1a1–14Zip in complex with WT and mutated Lmod2s1
Fragment
ΔTm (˚C)
P Value
Lmod2s1 [E34Q]
−2.9 ± 0.9
0.0228
Lmod2s1[S24K]
−1.4 ± 0.9
0.1588
Lmod2s1[S24L]
−2.8 ± 1.0
0.0583
Lmod2s1[E36V]
2.5 ± 0.8
0.0476
Lmod2s1Δ11
0.3
ND
Fragment
ΔTm (˚C)
P Value
Lmod2s1 [E34Q]
−2.9 ± 0.9
0.0228
Lmod2s1[S24K]
−1.4 ± 0.9
0.1588
Lmod2s1[S24L]
−2.8 ± 1.0
0.0583
Lmod2s1[E36V]
2.5 ± 0.8
0.0476
Lmod2s1Δ11
0.3
ND
To compare with WT, P values were calculated using an unpaired t test (n = 2, 2, 2, and 3 for S24K, S24L, E36V, and E34Q, respectively; n = 1 for Lmod2s1Δ11).