Table 2.

A list of antibodies used in immunofluorescence (IF) staining and immunoblotting (IB)

Associated figuresAssay typeProtein nameManufacturerCatalogue no.Dilution
Fig. 1 D  IF Nucleolin Bethyl A300–711A 1:1,000 
Fig. 1 J  IB UBE1 Abcam 10R-P104A 1:1,000 
Fig. 1 J  IB Sec61β A gift from Tom Rapoport lab (Harvard Medical School, Boston, MA, USA)  1:5,000 
Fig. 1, J and I; Fig. 2, C and D; Fig. 4, D and H  IB GFP Santa Cruz SC-9996 1:500 
Fig. 1 I; Fig. 2 C; Fig. 4, D and H  IB UFM1 Abcam Ab109305 1:1,000 
Fig. 1 I; Fig. 3 D  IB VCP/p97 Fitzgerald 10R-P104A 1:2,000 
Fig. 2, C and D  IB SAYSD1 Abcam Ab189918 1:1,000 
Fig. 2 D  IB RPL26 Bethyl A300–686A 1:1,000 
Fig. 3 D  IB Hrd1 Generated in-house Zhang et al. (2015)   1:1,000 
Fig. 4 A  IF GFP Thermo Fisher Scientific A11122 1:200 
Fig. 4 C  IB NEMF/SDCCAG1 Proteintech 11840-1-AP 1:1,000 
Fig. 4 C  IB LTN1/RNF160 Proteintech 28452-1-AP 1:2,000 
Fig. 4, C and H  IB HSP90 Santa Cruz SC-13119 1:1,000 
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