Table 3. Pairwise comparisons of Notch... | Rockefeller University Press

Table 3.

Pairwise comparisons of Notch^Y2328F distribution at late endosomal vesicles upon manipulations of Notch endocytic trafficking regulators

Condition (M/L ratio)	WT (1.21)	Abl OE (0.55)	Su(dx) OE (0.88)	Nedd4S OE (1.33)	Nedd4Lo OE (0.79)	Dx OE (2.94)	Nedd4 dsRNA 1 (1.49)	NYF (2.37)	NYF + Abl OE (2.47)	NYF + Su(dx) OE (2.58)	NYF + Nedd4Lo OE (1.75)
WT (1.21)	-	0.0002	0.0057	0.1244	0.0391	2.68 e-06	0.3606	8.81 e-06	3.52 e-08	1 e-07	0.0054
Abl OE (0.55)	0.0002	-	1.18 e-18	1.82 e-07	0.0641	6.26 e-14	1.6 e-05	2.83 e-06	1.48 e-18	6.10 e-16	1.92 e-09
Su(dx) OE (0.88)	0.0057	1.18 e-18	-	6.59 e-05	0.5859	8.53 e-11	0.0008	4.73 e-13	5.58 e-16	2.33 e-13	1.26 e-07
Nedd4S OE (1.33)	0.1244	1.82 e-07	6.59 e-05	-	0.0009	4.44 e-05	0.9430	0.0024	5.63 e-06	7 e-07	0.3218
Nedd4Lo OE (0.79)	0.0391	0.0641	0.5859	0.0009	-	1.11 e-07	0.0129	8.14 e-10	9.94 e-11	7.66 e-10	0.0004
Dx OE (2.94)	2.68 e-06	6.26 e-14	8.53 e-11	4.44 e-05	1.11 e-07	-	0.0002	0.1544	0.1916	0.5083	0.0018
Nedd4 dsRNA 1 (1.49)	0.3606	1.6 e-05	0.0008	0.9430	0.0129	0.0002	-	0.0099	5.26 e-05	1.90 e-06	0.2685
NYF (2.37)	8.81 e-06	2.83 e-06	4.73 e-13	0.0024	8.14 e-10	0.1544	0.0099	-	0.4710	0.0648	0.0020
NYF + Abl OE (2.47)	3.52 e-08	1.48 e-18	5.58 e-16	5.63 e-06	9.94 e-11	0.1916	5.26 e-05	0.4710	-	0.7203	0.0009
NYF + Su(dx) OE (2.58)	1 e-07	6.10 e-16	2.33 e-13	7 e-07	7.66 e-10	0.5083	1.90 e-06	0.0648	0.7203	-	0.0001
NYF + Nedd4Lo OE (1.75)	0.0054	1.92 e-09	1.26 e-07	0.3218	0.0004	0.0018	0.2685	0.0020	0.0009	0.0001	-

Condition (M/L ratio)	WT (1.21)	Abl OE (0.55)	Su(dx) OE(0.88)	Nedd4S OE (1.33)	Nedd4Lo OE (0.79)	Dx OE (2.94)	Nedd4 dsRNA 1 (1.49)	NYF (2.37)	NYF +Abl OE (2.47)	NYF +Su(dx) OE(2.58)	NYF +Nedd4Lo OE (1.75)
WT (1.21)	-	0.0002	0.0057	0.1244	0.0391	2.68 e-06	0.3606	8.81 e-06	3.52 e-08	1 e-07	0.0054
Abl OE (0.55)	0.0002	-	1.18 e-18	1.82 e-07	0.0641	6.26 e-14	1.6 e-05	2.83 e-06	1.48 e-18	6.10 e-16	1.92 e-09
Su(dx) OE(0.88)	0.0057	1.18 e-18	-	6.59 e-05	0.5859	8.53 e-11	0.0008	4.73 e-13	5.58 e-16	2.33 e-13	1.26 e-07
Nedd4S OE (1.33)	0.1244	1.82 e-07	6.59 e-05	-	0.0009	4.44 e-05	0.9430	0.0024	5.63 e-06	7 e-07	0.3218
Nedd4Lo OE (0.79)	0.0391	0.0641	0.5859	0.0009	-	1.11 e-07	0.0129	8.14 e-10	9.94 e-11	7.66 e-10	0.0004
Dx OE (2.94)	2.68 e-06	6.26 e-14	8.53 e-11	4.44 e-05	1.11 e-07	-	0.0002	0.1544	0.1916	0.5083	0.0018
Nedd4 dsRNA 1 (1.49)	0.3606	1.6 e-05	0.0008	0.9430	0.0129	0.0002	-	0.0099	5.26 e-05	1.90 e-06	0.2685
NYF (2.37)	8.81 e-06	2.83 e-06	4.73 e-13	0.0024	8.14 e-10	0.1544	0.0099	-	0.4710	0.0648	0.0020
NYF +Abl OE (2.47)	3.52 e-08	1.48 e-18	5.58 e-16	5.63 e-06	9.94 e-11	0.1916	5.26 e-05	0.4710	-	0.7203	0.0009
NYF +Su(dx) OE(2.58)	1 e-07	6.10 e-16	2.33 e-13	7 e-07	7.66 e-10	0.5083	1.90 e-06	0.0648	0.7203	-	0.0001
NYF +Nedd4Lo OE (1.75)	0.0054	1.92 e-09	1.26 e-07	0.3218	0.0004	0.0018	0.2685	0.0020	0.0009	0.0001	-

The average ratio of Notch signal at the membrane and at the lumen (M/L) of late endosomal compartments is shown in parenthesis for each individual genetic manipulation in S2 cells. A pairwise two-sided Kolmogorov–Smirnov test was performed, and P values are indicated. Sample size (# of LEs evaluated): Notch (WT, N = 38), Notch + Abl (N = 27), Notch + Su(dx) (N = 51), Notch + Nedd4S (N = 27), Notch + Nedd4Lo (N = 36), Notch + Dx (N = 68), Notch + Nedd4 dsRNA1 (N = 25), Notch^Y2328F (NYF, N = 43), Notch^Y2328F + Abl (N = 73), Notch^Y2328F + Su(dx) (N = 38), and Notch^Y2328F + Nedd4Lo (N = 52).

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