Table 2. Pairwise comparisons of Notch... | Rockefeller University Press

Table 2.

Pairwise comparisons of Notch distribution at late endosomal vesicles upon manipulations of Notch endocytic trafficking

Condition (M/L ratio)	WT (1.21)	LacZ dsRNA (1.22)	Abl dsRNA (1.69)	Imatinib (1.51)	Abl OE (0.55)	Abl^K417N OE (1.37)	Su(dx) dsRNA (2.18)	Su(dx) OE (0.88)	Abl dsRNA + Su(dx) dsRNA (2.42)	Abl OE + Su(dx) OE (0.76)	Abl OE + Su(dx) dsRNA (1.84)	Su(dx) OE + Abl dsRNA (0.96)
WT (1.21)	-	0.0425	0.0202	0.0019	0.0002	0.7132	7.86 e-07	0.0057	2.04 e-07	0.0440	0.0047	0.3806
LacZ dsRNA (1.22)	0.0425	-	0.2693	0.4104	5.07 e-06	0.6403	0.0001	0.0013	2.09 e-06	0.0132	0.1377	0.2739
Abl dsRNA (1.69)	0.0202	0.2693	-	0.0642	7.05 e-10	0.0168	0.0060	3.85 e-07	0.0027	4.33 e-05	0.7467	0.0066
Imatinib (1.51)	0.0019	0.4104	0.0642	-	1.20 e-11	0.0235	2.65 e-07	3.40 e-10	8.9 e-10	1.39 e-06	0.1219	0.0004
Abl OE (0.55)	0.0002	5.07 e-06	7.05 e-10	1.20 e-11	-	6.11 e-05	1.18 e-18	0.3060	3.70 e-15	0.1560	3.25 e-10	0.0006
Abl^K417N OE (1.37)	0.7132	0.6403	0.0168	0.0235	6.11 e-05	-	3.51 e-08	8.40 e-05	1.83 e-06	0.0019	0.0035	0.0482
Su(dx) dsRNA (2.18)	7.86 e-07	0.0001	0.0060	2.65 e-07	1.18 e-18	3.51 e-08	-	3.05 e-17	0.0193	1.04 e-08	0.0507	3.55 e-06
Su(dx) OE (0.88)	0.0057	0.0013	3.85 e-07	3.40 e-10	0.3060	8.40 e-05	3.05 e-17	-	4.99 e-13	0.7768	1 e-07	0.0144
Abl dsRNA + Su(dx) dsRNA (2.42)	2.04 e-07	2.09 e-06	0.0027	8.9 e-10	3.70 e-15	1.83 e-06	0.0193	4.99 e-13	-	4.22 e-08	0.0058	8.74 e-05
Abl OE + Su(dx) OE (0.76)	0.0440	0.0132	4.33 e-05	1.39 e-06	0.1560	0.0019	1.04 e-08	0.7768	4.22 e-08	-	4.77 e-05	0.0995
Abl OE + Su(dx) dsRNA (1.84)	0.0047	0.1377	0.7467	0.1219	3.25 e-10	0.0035	0.0507	1 e-07	0.0058	4.77 e-05	-	0.0036
Su(dx) OE + Abl dsRNA (0.96)	0.3806	0.2739	0.0066	0.0004	0.0006	0.0482	3.55 e-06	0.0144	8.74 e-05	0.0995	0.0036	-

Condition (M/L ratio)	WT (1.21)	LacZ dsRNA (1.22)	Abl dsRNA (1.69)	Imatinib (1.51)	Abl OE (0.55)	Abl^K417N OE (1.37)	Su(dx) dsRNA(2.18)	Su(dx) OE(0.88)	Abl dsRNA +Su(dx) dsRNA(2.42)	Abl OE +Su(dx) OE(0.76)	Abl OE +Su(dx) dsRNA(1.84)	Su(dx) OE +Abl dsRNA (0.96)
WT (1.21)	-	0.0425	0.0202	0.0019	0.0002	0.7132	7.86 e-07	0.0057	2.04 e-07	0.0440	0.0047	0.3806
LacZ dsRNA (1.22)	0.0425	-	0.2693	0.4104	5.07 e-06	0.6403	0.0001	0.0013	2.09 e-06	0.0132	0.1377	0.2739
Abl dsRNA (1.69)	0.0202	0.2693	-	0.0642	7.05 e-10	0.0168	0.0060	3.85 e-07	0.0027	4.33 e-05	0.7467	0.0066
Imatinib (1.51)	0.0019	0.4104	0.0642	-	1.20 e-11	0.0235	2.65 e-07	3.40 e-10	8.9 e-10	1.39 e-06	0.1219	0.0004
Abl OE (0.55)	0.0002	5.07 e-06	7.05 e-10	1.20 e-11	-	6.11 e-05	1.18 e-18	0.3060	3.70 e-15	0.1560	3.25 e-10	0.0006
Abl^K417N OE (1.37)	0.7132	0.6403	0.0168	0.0235	6.11 e-05	-	3.51 e-08	8.40 e-05	1.83 e-06	0.0019	0.0035	0.0482
Su(dx) dsRNA(2.18)	7.86 e-07	0.0001	0.0060	2.65 e-07	1.18 e-18	3.51 e-08	-	3.05 e-17	0.0193	1.04 e-08	0.0507	3.55 e-06
Su(dx) OE(0.88)	0.0057	0.0013	3.85 e-07	3.40 e-10	0.3060	8.40 e-05	3.05 e-17	-	4.99 e-13	0.7768	1 e-07	0.0144
Abl dsRNA +Su(dx) dsRNA(2.42)	2.04 e-07	2.09 e-06	0.0027	8.9 e-10	3.70 e-15	1.83 e-06	0.0193	4.99 e-13	-	4.22 e-08	0.0058	8.74 e-05
Abl OE +Su(dx) OE(0.76)	0.0440	0.0132	4.33 e-05	1.39 e-06	0.1560	0.0019	1.04 e-08	0.7768	4.22 e-08	-	4.77 e-05	0.0995
Abl OE +Su(dx) dsRNA(1.84)	0.0047	0.1377	0.7467	0.1219	3.25 e-10	0.0035	0.0507	1 e-07	0.0058	4.77 e-05	-	0.0036
Su(dx) OE +Abl dsRNA (0.96)	0.3806	0.2739	0.0066	0.0004	0.0006	0.0482	3.55 e-06	0.0144	8.74 e-05	0.0995	0.0036	-

The average ratio of Notch signal at the membrane and at the lumen (M/L ratio) of late endosomal compartments is shown in parenthesis for each individual genetic manipulation in S2 cells. A pairwise two-sided Kolmogorov–Smirnov test was performed, and P values are indicated. Sample size (# of LEs evaluated): Notch (WT, N = 38), LacZ dsRA (N = 25) Notch + Abl dsRNA (N = 32), Notch + imatinib (N = 50), Notch + Abl (N = 27), Notch + Abl^K417N (N = 52), Notch + Su(dx) dsRNA (N = 68), Notch + Su(dx) (N = 51), Notch + Abl dsRNA + Su(dx) dsRNA (N = 53), Notch + Abl + Su(dx) (N = 25), Notch + Abl + Su(dx) dsRNA (N = 27), and Notch + Su(dx) + Abl dsRNA (N = 47).

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