Table 1.

Antibodies used for flow cytometry

MarkerFluorophoreCloneManufacturer
CD45 AlexaFluor700 30-F11 BioLegend 
CD45 VF500 30-F11 Tonbo Biosciences 
F4/80 BV421 BM8 BioLegend 
F4/80 APC BM8 BioLegend 
F4/80 RedFluor710 BM8.1 Tonbo Biosciences 
CD11c BV605 N418 BioLegend 
Ly6C BV650 HK1.4 BioLegend 
CD11b BV711 M1/70 BioLegend 
CD8a BV785 53–6.7 BioLegend 
CD8a PE-Cy7 2.43 Tonbo Biosciences 
CD3e BUV395 145-2C11 BD Biosciences 
CD4 BUV563 GK1.5 BD Biosciences 
B220 BUV661 RA3–6B2 BD Biosciences 
Ly6G PE-CF594 1A8 BD Biosciences 
MHC-II PE-Cy7 M5/114.15.2 BioLegend 
MHC-II FITC M5/114.15.2 BioLegend 
CX3CR1 APC SA011F11 BioLegend 
CX3CR1 PE SA011F11 BioLegend 
CX3CR1 BV421 28–50 BD Biosciences 
Arg1 eFluor450 A1exF5 Invitrogen 
CD206 BV785 C068C2 BioLegend 
CD206 PE C068C2 BioLegend 
TNFα PerCPCy5.5 MP6-XT22 BioLegend 
Foxp3 APC FJK-16s Invitrogen 
CD25 BV421 PC61 BioLegend 
CD25 eFluor450 PC61.5 Invitrogen 
PD-1 BV605 29F.1A12 BioLegend 
Tim-3 APC RMT3–23 BioLegend 
TIGIT BV421 1G9 BioLegend 
IL17A BV650 TC11–18H10.1 BioLegend 
IL-4 BV786 11B11 BD Biosciences 
IFNγ BUV737 XMG1.2 BioLegend 
CD39 PE-Dazzle594 Duha59 BioLegend 
CD69 BV605 H1.2F3 BioLegend 
CD44 BV785 IM7 BioLegend 
CD62L BUV737 MEL-14 BD Biosciences 
NKp46 BV421 29A1.4 BioLegend 
MHC-I (H-2Kq) BV421 KH114 BD Biosciences 
MHC-I (H-2Dq, Lq) Biotin KH117 BD Biosciences 
CD16/32 N/A 2.4G2 Tonbo Biosciences 
Live/dead Fixable near-IR N/A Invitrogen 

Antibodies used for flow cytometry listed by marker, fluorophore, clone, and manufacturer.

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