Health of fluorescently tagged type IV collagen strains
| Strain | Fluorophore insertion site | Additional days to starvation relative to wild typea | ||
|---|---|---|---|---|
| Replicate 1 | Replicate 2 | Replicate 3 | ||
| let-2::mNG | C | 0 | 0 | 0 |
| let-2::mRuby2 | C | 0 | 0 | 0 |
| emb-9::mNG | C | 0 | 0 | 0 |
| emb-9::mRuby2 | C | 0 | 0 | 0 |
| emb-9::mRuby2; let-2::mNG | C; C | 0 | 0 | 0 |
| emb-9::mNG; let-2::mRuby2 | C; C | 0 | 0 | 0 |
| emb-9::mEos2 | C | 0 | 0 | 0 |
| let-2::mMaple | C | 1 | 1 | 1 |
| emb-9::mRuby2 | IS1 | 1 | 1 | 1 |
| emb-9::mScarlet-I | IS1 | 1 | 1 | 1 |
| emb-9::mCherry | IS2 | 2 | 2 | 2 |
| emb-9::mEos2 | IS1 | 5 | 5 | 5 |
| emb-9::mNG | IS1 | 6 | 8 | 8 |
| Strain | Fluorophore insertion site | Additional days to starvation relative to wild type | ||
|---|---|---|---|---|
| Replicate 1 | Replicate 2 | Replicate 3 | ||
| C | 0 | 0 | 0 | |
| C | 0 | 0 | 0 | |
| C | 0 | 0 | 0 | |
| C | 0 | 0 | 0 | |
| C; C | 0 | 0 | 0 | |
| C; C | 0 | 0 | 0 | |
| C | 0 | 0 | 0 | |
| C | 1 | 1 | 1 | |
| IS1 | 1 | 1 | 1 | |
| IS1 | 1 | 1 | 1 | |
| IS2 | 2 | 2 | 2 | |
| IS1 | 5 | 5 | 5 | |
| IS1 | 6 | 8 | 8 | |
For each strain, four L4 larvae were plated on 400 μl of OP50 E. coli and allowed to grow at 20°C (replicates of 3). With every batch of experiments, wild-type N2 animals were also plated. Plates were checked twice per day, and the time at which food was depleted was recorded (time to starvation). Numbers above indicate how much longer strains took to starve than N2 animals. No strains starved faster than N2 animals.
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