Oligonucleotides
| Primer name . | Primer sequence . | Purpose . |
|---|---|---|
| PBS2.XbaI.For | 5′-GATCtctagaACTAGAATGGAAGACAAGTTTGCTAA-3′ | PBS2 cloning |
| PBS2.Not1.Rev | 5′-GATCgcggccgcTAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| HOG1.XbaI.For | 5′-GATCtctagaACTAGAATGACCACTAACGAGGAATT-3′ | HOG1 cloning |
| HOG1.Not1.Rev | 5′-GATCgcggccgcCTGTTGGAACTCATTAGCGTACT-3′ | HOG1 cloning |
| JLP1.XbaI.For | 5′-GATCtctagaATGTCTCCTGCAGCAGCTCAAACAGCA-3′ | JLP1 cloning |
| JLP1.Not1.Rev | 5′-GATCgcggccgcAATATCCAAAGTTAATGAAGAAGGA-3′ | JLP1 cloning |
| SAM1.XbaI.For | 5′-GATCtctagaATGGCCGGTACATTTTTATTC-3′ | SAM1 cloning |
| SAM1.Not1.Rev | 5′-GATCgcggccgcGAACTTCAAAGTCTTAGGCT-3′ | SAM1 cloning |
| KAP123.XbaI.For | 5′-GATCtctagaATGGATCAACAATTTCTAAG-3′ | KAP123 cloning |
| KAP123.Not1.Rev | 5′-GATCgcggccgcAGCAATGACGGCAGCTAAAAC-3′ | KAP123 cloning |
| pMET25.DUR12.start.For | 5′-ATACAATTCTATTACCCCCATCCATACTCTAGAACTAGAATGACAGTTAGTTCCGATAC-3′ | DUR12 cloning |
| pMET25.DUR12.start.Rev | 5′-CTAACGATATTTCAGCAGTTGTATCGGAACTAACTGTCATTCTAGTTCTAGAGTATGGAT-3′ | DUR12 cloning |
| pMET25.start.EcoR1 | 5′-GATCgaattcAGCTCCGGATGCAAGGGTTC-3′ | DUR12 cloning |
| DUR12.Not1.Rev | 5′-GATCgcggccgcTGCCAATGTCTCTATGACGGCCACT-3′ | DUR12 cloning |
| PBS2.Sma1.Rev | 5′-GATCcccgggATAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| pMET25.start.HindIII | 5′-GATCaagcttAGCTCCGGATGCAAGGGTTC-3′ | HOG1 cloning |
| HOG1.BamH1.Xba1.Rev | 5′-GATCggatcctctagaGCCTGTTGGAACTCATTAGCGTACT-3′ | HOG1 cloning |
| pPAB1.SacI.For | 5′-GATCgagctcTAGATCAATCATGTTTAACG-3′ | PAB1 cloning |
| PAB1.Pac1.Rev | 5′-GATCttaattaaAGCTTGCTCAGTTTGTTGTTC-3′ | PAB1 cloning |
| pHOG1.Sac1.For | 5′-GATCgagctcATCGATTGAAGGAAATAAGAGGAAT-3′ | HOG1 cloning |
| GFP.Not1.Rev | 5′-GATCgcggccgcCTTCACATGTTCCGCAGAT-3′ | HOG1 cloning |
| pMET25.Sac1.For | 5′-GATCgagctcAGCTCCGGATGCAAGGGTTC-3′ | PBS2 cloning |
| PBS2.BamH1.Rev | 5′-GATCggatccATAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| JLP1.BspE1.Rev | 5′-GATCtccggaAATATCCAAAGTTAATGAAGAAGGA-3′ | JLP1 cloning |
| PBS2-S514DT518D | 5′-CGGTGTTTCTGGTAATTTGGTGGCAGATTTAGCGAAGGATAATATTGGTTGTCAGTCATACATG-3′ | PBS2-DD cloning |
| PBS2-S514DT518D.anti | 5′-CATGTATGACTGACAACCAATATTATCCTTCGCTAAATCTGCCACCAAATTACCAGAAACACCG-3′ | PBS2-DD cloning |
| HOG1-DADA | 5′-GCTCCTTACCACGCTCCAACGGCTGAACCAGTAGCC-3′ | HOG1-DADA cloning |
| HOG1-DADA.anti | 5′-GGCTACTGGTTCAGCCGTTGGAGCGTGGTAAGGAGC-3′ | HOG1-DADA cloning |
| Primer name . | Primer sequence . | Purpose . |
|---|---|---|
| PBS2.XbaI.For | 5′-GATCtctagaACTAGAATGGAAGACAAGTTTGCTAA-3′ | PBS2 cloning |
| PBS2.Not1.Rev | 5′-GATCgcggccgcTAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| HOG1.XbaI.For | 5′-GATCtctagaACTAGAATGACCACTAACGAGGAATT-3′ | HOG1 cloning |
| HOG1.Not1.Rev | 5′-GATCgcggccgcCTGTTGGAACTCATTAGCGTACT-3′ | HOG1 cloning |
| JLP1.XbaI.For | 5′-GATCtctagaATGTCTCCTGCAGCAGCTCAAACAGCA-3′ | JLP1 cloning |
| JLP1.Not1.Rev | 5′-GATCgcggccgcAATATCCAAAGTTAATGAAGAAGGA-3′ | JLP1 cloning |
| SAM1.XbaI.For | 5′-GATCtctagaATGGCCGGTACATTTTTATTC-3′ | SAM1 cloning |
| SAM1.Not1.Rev | 5′-GATCgcggccgcGAACTTCAAAGTCTTAGGCT-3′ | SAM1 cloning |
| KAP123.XbaI.For | 5′-GATCtctagaATGGATCAACAATTTCTAAG-3′ | KAP123 cloning |
| KAP123.Not1.Rev | 5′-GATCgcggccgcAGCAATGACGGCAGCTAAAAC-3′ | KAP123 cloning |
| pMET25.DUR12.start.For | 5′-ATACAATTCTATTACCCCCATCCATACTCTAGAACTAGAATGACAGTTAGTTCCGATAC-3′ | DUR12 cloning |
| pMET25.DUR12.start.Rev | 5′-CTAACGATATTTCAGCAGTTGTATCGGAACTAACTGTCATTCTAGTTCTAGAGTATGGAT-3′ | DUR12 cloning |
| pMET25.start.EcoR1 | 5′-GATCgaattcAGCTCCGGATGCAAGGGTTC-3′ | DUR12 cloning |
| DUR12.Not1.Rev | 5′-GATCgcggccgcTGCCAATGTCTCTATGACGGCCACT-3′ | DUR12 cloning |
| PBS2.Sma1.Rev | 5′-GATCcccgggATAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| pMET25.start.HindIII | 5′-GATCaagcttAGCTCCGGATGCAAGGGTTC-3′ | HOG1 cloning |
| HOG1.BamH1.Xba1.Rev | 5′-GATCggatcctctagaGCCTGTTGGAACTCATTAGCGTACT-3′ | HOG1 cloning |
| pPAB1.SacI.For | 5′-GATCgagctcTAGATCAATCATGTTTAACG-3′ | PAB1 cloning |
| PAB1.Pac1.Rev | 5′-GATCttaattaaAGCTTGCTCAGTTTGTTGTTC-3′ | PAB1 cloning |
| pHOG1.Sac1.For | 5′-GATCgagctcATCGATTGAAGGAAATAAGAGGAAT-3′ | HOG1 cloning |
| GFP.Not1.Rev | 5′-GATCgcggccgcCTTCACATGTTCCGCAGAT-3′ | HOG1 cloning |
| pMET25.Sac1.For | 5′-GATCgagctcAGCTCCGGATGCAAGGGTTC-3′ | PBS2 cloning |
| PBS2.BamH1.Rev | 5′-GATCggatccATAAACCACCCATATGTAATG-3′ | PBS2 cloning |
| JLP1.BspE1.Rev | 5′-GATCtccggaAATATCCAAAGTTAATGAAGAAGGA-3′ | JLP1 cloning |
| PBS2-S514DT518D | 5′-CGGTGTTTCTGGTAATTTGGTGGCAGATTTAGCGAAGGATAATATTGGTTGTCAGTCATACATG-3′ | PBS2-DD cloning |
| PBS2-S514DT518D.anti | 5′-CATGTATGACTGACAACCAATATTATCCTTCGCTAAATCTGCCACCAAATTACCAGAAACACCG-3′ | PBS2-DD cloning |
| HOG1-DADA | 5′-GCTCCTTACCACGCTCCAACGGCTGAACCAGTAGCC-3′ | HOG1-DADA cloning |
| HOG1-DADA.anti | 5′-GGCTACTGGTTCAGCCGTTGGAGCGTGGTAAGGAGC-3′ | HOG1-DADA cloning |
Restrictions sites are in lower case.