Plasmids
| Plasmid . | Description . | Source or reference . |
|---|---|---|
| p115 | pRS316 | Sikorski and Hieter (1989) |
| p1105 | pRS425 | Sikorski and Hieter (1989) |
| p1230 | pYES2-GAL1P-STE11-N∆ | pNC544, Posas and Saito (1997) |
| p2823 | pAG32 | Goldstein and McCusker (1999) |
| p3121 | YEp181-MET25P-FPS1-Myc | Lee et al. (2013) |
| p3148 | pRS316-3HA-ADH1T | Lee et al. (2013) |
| p3151 | pRS316-MET25P-RGC2-3HA | Lee et al. (2013) |
| p3177 | pRS416-HOG1-GFP | Ferrigno et al. (1998) |
| p3199 | pRS413-CFP-C | Lipatova et al. (2012) |
| p3200 | pRS415-CFP-N | Lipatova et al. (2012) |
| p3217 | pRS413-CFP-C-HOG1 (BiFC) | Lee et al. (2013) |
| p3261 | pFA6a-KanMX6 | Longtine et al. (1998) |
| p3263 | pFA6a-HIS3MX6 | Longtine et al. (1998) |
| p3370 | pYES2-GAL1P-PBS2-DD | pGPBD21, Wurgler-Murphy et al. (1997) |
| p3454 | pUT36- MET25P-HOG1-HIS6 | Lee and Levin (2018) |
| p3649 | GAL1P-GST-SEC53 | Open Biosystems |
| p3651 | GAL1P-JLP1-TAP | Open Biosystems |
| p3652 | GAL1P-KAP123-TAP | Open Biosystems |
| p3668 | TDH3-mCherry vector, URA3 | pRP1669, Roy Parker, University of Colorado, Boulder, CO, USA |
| p3670 | PAB1-GFP, HIS3 | pRP2477, Roy Parker, University of Colorado, Boulder, CO, USA |
| p3671 | pRS316-MET25P-PBS2-3HA | This study |
| p3672 | pRS316-MET25P-HOG1-3HA | This study |
| p3673 | YEp181-MET25P-HOG1-Myc | This study |
| p3674 | YEp181-MET25P-PBS2-Myc | This study |
| p3675 | pRS316-MET25P-PBS2-S514DT518D-(DD)-3HA | This study |
| p3676 | pRS316-MET25P-JLP1-3HA | This study |
| p3677 | pRS316-MET25P-SAM1-3HA | This study |
| p3678 | pRS316-MET25P-KAP123-3HA | This study |
| p3679 | pRS316-MET25P-DUR12-3HA | This study |
| p3680 | pRS425-HOG1-GFP | This study |
| p3681 | pRP1669-PAB1-mCherry (URA3) | This study |
| p3683 | pRS415-JLP1-CFP-N (BiFC) | This study |
| p3685 | YEp181-MET25P-HOG1-D307A/D310A-Myc (HOG1-DADA) | This study |
| p3686 | pRP1669-MET25P-PBS2-mCherry (URA3) | This study |
| Plasmid . | Description . | Source or reference . |
|---|---|---|
| p115 | pRS316 | Sikorski and Hieter (1989) |
| p1105 | pRS425 | Sikorski and Hieter (1989) |
| p1230 | pYES2-GAL1P-STE11-N∆ | pNC544, Posas and Saito (1997) |
| p2823 | pAG32 | Goldstein and McCusker (1999) |
| p3121 | YEp181-MET25P-FPS1-Myc | Lee et al. (2013) |
| p3148 | pRS316-3HA-ADH1T | Lee et al. (2013) |
| p3151 | pRS316-MET25P-RGC2-3HA | Lee et al. (2013) |
| p3177 | pRS416-HOG1-GFP | Ferrigno et al. (1998) |
| p3199 | pRS413-CFP-C | Lipatova et al. (2012) |
| p3200 | pRS415-CFP-N | Lipatova et al. (2012) |
| p3217 | pRS413-CFP-C-HOG1 (BiFC) | Lee et al. (2013) |
| p3261 | pFA6a-KanMX6 | Longtine et al. (1998) |
| p3263 | pFA6a-HIS3MX6 | Longtine et al. (1998) |
| p3370 | pYES2-GAL1P-PBS2-DD | pGPBD21, Wurgler-Murphy et al. (1997) |
| p3454 | pUT36- MET25P-HOG1-HIS6 | Lee and Levin (2018) |
| p3649 | GAL1P-GST-SEC53 | Open Biosystems |
| p3651 | GAL1P-JLP1-TAP | Open Biosystems |
| p3652 | GAL1P-KAP123-TAP | Open Biosystems |
| p3668 | TDH3-mCherry vector, URA3 | pRP1669, Roy Parker, University of Colorado, Boulder, CO, USA |
| p3670 | PAB1-GFP, HIS3 | pRP2477, Roy Parker, University of Colorado, Boulder, CO, USA |
| p3671 | pRS316-MET25P-PBS2-3HA | This study |
| p3672 | pRS316-MET25P-HOG1-3HA | This study |
| p3673 | YEp181-MET25P-HOG1-Myc | This study |
| p3674 | YEp181-MET25P-PBS2-Myc | This study |
| p3675 | pRS316-MET25P-PBS2-S514DT518D-(DD)-3HA | This study |
| p3676 | pRS316-MET25P-JLP1-3HA | This study |
| p3677 | pRS316-MET25P-SAM1-3HA | This study |
| p3678 | pRS316-MET25P-KAP123-3HA | This study |
| p3679 | pRS316-MET25P-DUR12-3HA | This study |
| p3680 | pRS425-HOG1-GFP | This study |
| p3681 | pRP1669-PAB1-mCherry (URA3) | This study |
| p3683 | pRS415-JLP1-CFP-N (BiFC) | This study |
| p3685 | YEp181-MET25P-HOG1-D307A/D310A-Myc (HOG1-DADA) | This study |
| p3686 | pRP1669-MET25P-PBS2-mCherry (URA3) | This study |