Table 1.

Resources

Reagent or ResourceSourceIdentifier
Antibodies 
N-term CIZ1 Rabbit pAb Coverley et al. (2005)  1793 
C-term CIZ1 Mouse mAb Ex17 FDAB 87 Sofi et al. (2022)  
CIZ1 Rabbit pAb Ex17 Novus NB100-74624 
Rabbit H3K27me3 CST 9733S 
Rabbit H2AK119ub1 CST 8240 
Goat α Rabbit Alexa Fluor 568 (Red) Invitrogen A11011 
Goat α Mouse Alexa Fluor 488 (Green) Invitrogen A11001 
Goat α Rabbit Alexa Fluor 488 (Green) Invitrogen A11034 
Goat α Mouse Alexa Fluor 568 (Red) Invitrogen A11031 
Histone H3 Abcam ab1791 
β-Actin Abcam ab11003 
Peroxidase IgG α Rabbit Jackson 211-032-171 
Peroxidase IgG α Mouse Jackson 155-035-174 
Bacterial and virus strains 
Escherichia coli DH5α competent cells Invitrogen Cat#18265017 
Escherichia coli Stbl3 competent cells Invitrogen Cat#C737303 
Chemicals, peptides, and recombinant proteins 
MEBM basal medium Lonza Cat#CC-3150 
MEGM SingleQuots Lonza Cat#CC-4136 
HG DMEM Gibco Cat#31966-021 
Collagen Type I, Rat tail Thistle Scientific Cat#50201 
X2 Transfection reagent Mirus Cat#MIR 6003 
PR-619 Bio-Techne Cat#4482/10 
Nocodazole Sigma-Aldrich Cat#M1404 
Thymidine Sigma-Aldrich Cat#T1895 
PolyFect transfection reagent QIAGEN Cat#301105 
CloneAmp HiFi PCR premix Takara Cat#639298 
QIAprep Spin Miniprep Kit QIAGEN Cat#27104 
Protease inhibitor cocktail, EDTA-free BioVision Cat#K272-1 
PreScission protease GE Healthcare Cat#27084301 
Critical commercial assays 
TissueScan cancer and normal tissue cDNA arrays Origene https://www.origene.com/products/tissues/tissuescan 
Deposited data   
Murine cell transcriptomes BMK gene National Center for Biotechnology Information short Read archive project PRJNA1144651 
Experimental models: Cells   
Mouse: Female 3T3 fibroblast cell line Stewart et al. (2019)  D3T3 
Mouse: Primary embryonic fibroblasts (PEFs) Ridings-Figueroa et al. (2017) and this study Strain specific identifiers 
Human: Primary mammary epithelial cells Breast Cancer now HMEC 
Human: MCF-10A cell line ATCC CRL-10317 
Human: MCF7 cell line ECACC 86012803 
Human: BT-474 cell line TCBSR BT-474 
Human: SK-BR-3 cell line TCBSR SK-BR-3 
Human: MDA-MB-231 cell line ATCC HTB-26 
Human: Lenti-X 293T cell line Takara Cat#632180 
Experimental models: Organisms/strains 
Ciz1 null mice Ciz1Gt(IST13830B6)Tigm Ridings-Figueroa et al. (2017)  N/A 
Oligonucleotides 
qPCR primers   
5′-CAG​GGG​CAT​AAG​GAC​AAA​G-3′ CIZ1 13F P1 
5′-TCC​GAG​CCC​TTC​CAC​TCC​TCT​CTG​G-3′ CIZ1 15R P2 
5′-CGA​GGG​TGA​TGA​AGA​AGA​GGA-3′ CIZ1 14F P6 
5′-CCC​CTG​AGT​TGC​TGT​GAT​A-3′ CIZ1 16R P7 
5′-CAC​AAC​TGG​CCA​CTC​CAA​AT-3′ CIZ1 5F P9 
5′-CCT​CTA​CCA​CCC​CCA​ATC​G-3′ CIZ1 5R P10 
5′-ACA​CAC​CAG​AAG​ACC​AAG​ATT​TAC​C-3′ CIZ1 6/7 junction F P13 
5′-TGC​TGG​AGT​GCG​TTT​TTC​CT-3′ CIZ1 7R P14 
5′-CAA​CCG​CGA​GAA​GAT​GAC​C-3′  Actin F 
5′-TCC​AGG​GCG​ACG​TAG​CAC​A-3′  Actin R 
qPCR probes   
5′-CGC​CAG​TCC​TTG​CTG​GGA​CC-3′ Ciz1 5 5 
5′-CCC​TGC​CCA​GAG​GAC​ATC​GCC-3′ Ciz1 7 7 
5′-TGG​TCC​TCA​TCT​TGG​CCA​GCA-3′ Ciz1 14 14 
5′-CAC​GGG​CAC​CAG​GAA​GTC​CA-3′ Ciz1 16 16 
5′-CCC​TGT​ACG​CCT​CTG​GCC​GT-3′  Actin 
Mutagenesis primers  Plasmid generated 
FP - 5′-GGA​GAG​ATT​GAG​GTG​AAG​CCG​AGA​GAA​ACA​TCC-3′  GFP-C181 Δ706–713 (CIZ1B) 
RP - 5′-GGA​TGT​TTC​TCT​CGG​CTT​CAC​CTC​AAT​CTC​TCC-3′   
FP - 5′-GGA​TTT​CCT​GGT​GCC​AGT​GAT​GAA​AGC​CAA​GAA​CCC​AAG​C  GFP-C181 Δ746–779 (ΔMH3) 
RP - 5′-GCT​TGG​GTT​CTT​GGC​TTT​CAT​CAC​TGG​CAC​CAG​GAA​ATC​C-3′   
FP - 5′-CCT​GAC​TGC​ACT​GTT​CTG​ATA​GAA​GCT​TCG​AAT​TCT​GC-3′  GFP-C181 Δ809–845 (Δ37) 
RP - 5′-GCA​GAA​TTC​GAA​GCT​TCT​ATC​AGA​ACA​GTG​CAG​TCA​GG-3′   
FP - 5′-GCC​CTC​CTC​CTA​CCA​GCC​ACC​AGC​CCA​GCC-3′  GFP-C181 Δ796–811 (ΔNALTAF) 
RP - 5′-GGC​TGG​GCT​GGT​GGC​TGG​TAG​GAG​GAG​GGC-3′   
FP - 5′-ATC​CCC​GAA​TTC​CCG​GGT​CGA​CAA​GGA​GAC​AGG​CAG​CCC  GST-C181 from GST-C275 
RP - 5′-GGG​CTG​CCT​GTC​TCC​TTG​TCG​ACC​CGG​GAA​TTC​GGG​GAT-3′   
FP- 5′-GCT​TTG​AGA​GTG​GTC​AAT​TCT​GCA​AGC​AGG​TGA​AGC-3′  GST-C181 Δ689–709 (ΔAcD) 
RP- 5′-GCT​TCA​CCT​GCT​TGC​AGA​ATT​GAC​CAC​TCT​CAA​AGC-3′   
FP - 5′-GGA​TTT​CCT​GGT​GCC​AGT​GAT​GAA​AGC​CAA​GAA​CCC​AAG​C-3′  GST- C181 Δ746–779 (ΔMH3) 
RP - 5′-GCT​TGG​GTT​CTT​GGC​TTT​CAT​CAC​TGG​CAC​CAG​GAA​ATC​C-3′   
FP - 5′-CCT​GAC​TGC​ACT​GTT​CTG​ATA​GAG​GGA​GC-3′  GST-C181 Δ809–845 (Δ37) 
RP - 5′-GCT​CCC​TCT​ATC​AGA​ACA​GTG​CAG​TCA​GG-3′   
5′-AGA​CAG​GCA​GCC​CAG​ATG​AGG-3′  Sequencing primer 
Recombinant plasmids 
Murine GFP-C275 Ainscough et al. (2007)  N/A 
Murine GFP-C181 This paper N/A 
Murine GFP-C181 CIZ1B This paper N/A 
Murine GFP-C181 ΔMH3 This paper N/A 
Murine GFP-C181 ΔNALTALF This paper N/A 
Murine GFP-C181 Δ37 This paper N/A 
Murine GFP-C181 Δ37, ΔMH3 This paper N/A 
Murine GFP-I122 Ainscough et al. (2007)  N/A 
psPAX2 Trono Lab unpublished Addgene plasmid #12260 
pMD2.G Trono Lab unpublished Addgene plasmid #12259 
pLVX-EF1α-IRES-ZsGreen1 Takara Cat#631982 
Software and algorithms 
Gene expression profiling Interactive analysis (GEPIA) Tang et al. (2017)  http://gepia.cancer-pku.cn/ 
The cancer genome atlas (TCGA)  https://portal.gdc.cancer.gov/ 
UCSC genome browser Kent et al. (2002)  https://genome.ucsc.edu/ 
Catalogue of somatic Mutations In Cancer (COSMIC) Tate et al. (2019)  https://www.sanger.ac.uk/tool/cosmic/ 
Ensembl genome browser Cunningham et al. (2022)  https://www.ensembl.org/index.html 
Functional annotation of the mammalian genome (FANTOM5) Lizio et al. (2015)  https://fantom.gsc.riken.jp/5/ 
Cellosaurus Bairoch (2018)  https://www.cellosaurus.org/ 
GSEAPY Fang et al. (2023)  https://gseapy.readthedocs.io/en/latest/introduction.html 
FIJI Schindelin et al. (2012)  https://imagej.net/software/fiji/ 
IBM SPSS Statistics for Macintosh, version 28.0 IBM N/A 
nfcore/atacseq v2.1.2 workflow Zenodo https://doi.org/10.5281/zenodo.8222875 
LCsolution software Shimadzu N/A 
Astra V software Wyatt N/A 
PXi GenSys software Syngene N/A 
EVOS Xl digital inverted microscope software AMG (now Thermo Fisher Scientific) N/A 
Axiovision image acquisition software (SE64 release 4.9.1) Zeiss N/A 
CLASTR Bairoch (2018)  https://web.expasy.org/cellosaurus-str-search/ 
GSEA Broad Institute https://www.gsea-msigdb.org/gsea/index.jsp 
National center for biotechnology information genes and disease  https://www.ncbi.nlm.nih.gov/books/NBK22266/ 
[ViewLarge]
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Please sign in to your personal account to gift article access.

Register

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Gift articles remaining: --

Gift article access

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Gift articles remaining: --

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses.

You have reached the limit of 10 links within a 30 day period.

Sharing Unavailable

Sharing content requires targeting cookies to be enabled. Please update your cookie preferences to use this feature.