Orientations and relative angle changes of the four conformational states of AdiC
| . | θ1 Ex . | θ2 Eo . | θ3 Io . | θ4 Ix . |
|---|---|---|---|---|
| Crystallographya | 93.8° | 83.6° | 71.7° | 95.3° |
| Polarization (BFb, Agm2+) | 90° ± 5.3° | 88.7° ± 6.7° | 77.4° ± 9.3° | 90° ± 5.3° |
| Polarization (BFb, Arg+) | 90° ± 4.7° | 87.8° ± 7.2° | 76.6° ± 9.0° | 90° ± 4.5° |
| Polarization (MFc, Arg+) | 90° ± 4.6° | 89.0° ± 5.5° | 79.9° ± 7.3° | 90° ± 4.1° |
| φ1 Ex | φ2 Eo | φ3 Io | φ4 Ix | |
| Crystallographya | −1.89° | 20.9° | 27.5° | 38.7° |
| Polarization (BFb, Agm2+) | 0° ± 4.5° | 17.9° ± 6.3° | 27.2° ± 9.3° | 40.5° ± 10° |
| Polarization (BFb, Arg+) | 0° ± 4.0° | 16.6° ± 7.2° | 26.6° ± 10° | 38.5° ± 11° |
| Polarization (MFc, Arg+) | 0° ± 3.9° | 13.7° ± 4.8° | 22.7° ± 6.7° | 39.5° ± 7.5° |
| . | θ1 Ex . | θ2 Eo . | θ3 Io . | θ4 Ix . |
|---|---|---|---|---|
| Crystallographya | 93.8° | 83.6° | 71.7° | 95.3° |
| Polarization (BFb, Agm2+) | 90° ± 5.3° | 88.7° ± 6.7° | 77.4° ± 9.3° | 90° ± 5.3° |
| Polarization (BFb, Arg+) | 90° ± 4.7° | 87.8° ± 7.2° | 76.6° ± 9.0° | 90° ± 4.5° |
| Polarization (MFc, Arg+) | 90° ± 4.6° | 89.0° ± 5.5° | 79.9° ± 7.3° | 90° ± 4.1° |
| φ1 Ex | φ2 Eo | φ3 Io | φ4 Ix | |
| Crystallographya | −1.89° | 20.9° | 27.5° | 38.7° |
| Polarization (BFb, Agm2+) | 0° ± 4.5° | 17.9° ± 6.3° | 27.2° ± 9.3° | 40.5° ± 10° |
| Polarization (BFb, Arg+) | 0° ± 4.0° | 16.6° ± 7.2° | 26.6° ± 10° | 38.5° ± 11° |
| Polarization (MFc, Arg+) | 0° ± 3.9° | 13.7° ± 4.8° | 22.7° ± 6.7° | 39.5° ± 7.5° |
In the structural analysis, the AdiC structures in the states EO (PDB: 7O82) and EX (PDB: 3L1L) and the BasC and ApcT structures in the states IO (PDB: 6F2G) and IX (PDB: 3GIA) were used. Data are shown as mean ± σ.
“BF” indicates the fluorophore rhodamine bifunctionally attached.
“MF” indicates the fluorophore ATTO-550 monofunctionally attached.