Cryo-EM data collection, refinement, and validation statistics
| Dataset . | F-tractin-F-actin complex . | F-tractinopt-F-actin complex . |
|---|---|---|
| Microscope | Talos Arctica | |
| Voltage (kV) | 200 | |
| Defocus range (µm) | −0.5 to −2.5 | |
| Camera | Gatan K3 Superresolution mode | |
| Pixel size (Å) | 1.04 | |
| Total electron dose (e/Å2) | 45 | 55 |
| Exposure time (s) | 3.5 | 4 |
| Frames per movie | 50 | 50 |
| Number of movies | 3,391 (8,100) | 3,681 (4,222) |
| 3D Refinement | ||
| Number of particles | 265,256 | 298,240 |
| Final resolution (Å) | 3.2 | 3.4 |
| Helical rise (Å) | 27.4 | 27.4 |
| Helical twist (°) | −166.6 | −166.6 |
| Atomic model statistics | ||
| Non-hydrogen atoms | 14,815 | 14,798 |
| Molprobity score | 1.53 | 1.47 |
| Rama distribution Z-score | −1.88 ± 0.17 | −1.83 ± 0.18 |
| Clashscore | 4.96 | 4.42 |
| Bond RMSD (Å) | 0.003 | 0.003 |
| Angle RMSD (°) | 0.732 | 0.726 |
| Cβ deviations >0.25 Å | 0 | 0 |
| Poor rotamers (%) | 0 | 0 |
| Favored rotamers (%) | 99.62 | 99.62 |
| Ramachandran favored (%) | 96.11 | 96.27 |
| Ramachandran allowed (%) | 3.89 | 3.79 |
| Ramachandran outliers (%) | 0 | 0 |
| Dataset . | F-tractin-F-actin complex . | F-tractinopt-F-actin complex . |
|---|---|---|
| Microscope | Talos Arctica | |
| Voltage (kV) | 200 | |
| Defocus range (µm) | −0.5 to −2.5 | |
| Camera | Gatan K3 Superresolution mode | |
| Pixel size (Å) | 1.04 | |
| Total electron dose (e/Å2) | 45 | 55 |
| Exposure time (s) | 3.5 | 4 |
| Frames per movie | 50 | 50 |
| Number of movies | 3,391 (8,100) | 3,681 (4,222) |
| 3D Refinement | ||
| Number of particles | 265,256 | 298,240 |
| Final resolution (Å) | 3.2 | 3.4 |
| Helical rise (Å) | 27.4 | 27.4 |
| Helical twist (°) | −166.6 | −166.6 |
| Atomic model statistics | ||
| Non-hydrogen atoms | 14,815 | 14,798 |
| Molprobity score | 1.53 | 1.47 |
| Rama distribution Z-score | −1.88 ± 0.17 | −1.83 ± 0.18 |
| Clashscore | 4.96 | 4.42 |
| Bond RMSD (Å) | 0.003 | 0.003 |
| Angle RMSD (°) | 0.732 | 0.726 |
| Cβ deviations >0.25 Å | 0 | 0 |
| Poor rotamers (%) | 0 | 0 |
| Favored rotamers (%) | 99.62 | 99.62 |
| Ramachandran favored (%) | 96.11 | 96.27 |
| Ramachandran allowed (%) | 3.89 | 3.79 |
| Ramachandran outliers (%) | 0 | 0 |