Key resources table
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Rabbit anti-ARR3 | Millipore Sigma | Cat#AB15282; RRID:AB_ 1163387 |
| Goat anti-Bhlhb5 | Santa Cruz | Cat#sc-6045; RRID:AB_2065343 |
| Mouse anti-CTBP2 | BD Biosciences | Cat#612044; RRID:AB_399431 |
| Rabbit anti-dsRed | Clontech | Cat#632496; RRID:AB_10013483 |
| Mouse anti-FAT3 | Avilés et al. (2022), Deans et al. (2011) | N/A; RRID:AB_2904260 |
| Chicken anti-GFP | Aves | Cat#GFP-1020; RRID:AB_10000240 |
| Mouse anti-GRIK1 (GluR5) | Santa Cruz Biotechnology | Cat#sc-393420; RRID:AB_2716684 |
| Sheep anti-GRM6 | Cao et al. (2009) | N/A |
| Goat anti-PTPσ | R&D Systems | Cat#AF3430; RRID:AB_2175157 |
| Mouse anti-PTPσ | Medimabs | Cat#MM-0020-P |
| Rabbit anti-VGAT | Synaptic systems | Cat#131002; RRID:AB_887871 |
| Mouse anti-VSX2 (Chx10) | Santa Cruz Biotechnology | Cat#sc-365519; RRID:AB_10842442 |
| Donkey anti-chicken, Alexa Fluor 488 | Jackson ImmunoResearch | Cat#703-545-155; RRID:AB_2340375 |
| Donkey anti-goat, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A11057; RRID:AB_142581 |
| Donkey anti-mouse, Alexa Fluor 488 | Abcam | Cat#ab150105; RRID:AB_2732856 |
| Donkey anti-mouse, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A10037; RRID:AB_2534013 |
| Goat anti-mouse, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A-21235; RRID:AB_2535804 |
| Donkey anti-rabbit, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A21206; RRID:AB_2535792 |
| Donkey anti-rabbit, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A10042; RRID:AB_2534017 |
| Donkey anti-rabbit, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A31573; RRID:AB_2536183 |
| Donkey anti-sheep, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A-21099; RRID:AB_2535753 |
| Goat anti-mouse – HRP | BioRad | Cat#170-6516; RRID:AB_11125547 |
| Commercial assays | ||
| RNAscope Multiplex Fluorescent Reagent Kit v2 | ACD | Cat#323100 |
| HCR IHC Bundle | Molecular Instruments | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: Ptf1aCRE | C. Wright, Vanderbilt U (Fujitani et al., 2006) | MGI:2387812 |
| Mouse: Isl1CRE | The Jackson Laboratory (Yang et al., 2006) | Strain #: 024242 |
| Mouse: Bhlhe22CRE | M.E. Greenberg (Harvard Medical School) (Ross et al., 2010) | N/A |
| Mouse: Fat3floxed | Deans et al. (2011) | N/A |
| Mouse: Fat3∆TM | Deans et al. (2011) | N/A |
| Mouse: Fat3∆ICD-GFP | Avilés et al. (2022) | N/A |
| Mouse: Grik1−/− | Christophe Mulle (University of Bordeaux) (Mulle et al., 2000) | N/A |
| Mouse: Grm6−/− | The Jackson Laboratory (Maddox et al., 2008) | Strain #: 016883 |
| Mouse: Ptprs−/− | Michel Tremblay (Elchebly et al., 1999) | MGI:2158757 |
| In situ hybridization probes | ||
| RNAscope Probe- Mm-Fat3-O1 | ACD | Cat#509051 |
| RNAscope Probe- Mm-Grik1-C3 | ACD | Cat#438771-C3 |
| Recombinant DNA | ||
| AAV-Grik1-GFP plasmid | This paper | N/A |
| Viral vectors | ||
| AAV8-Grik1-GFP | This paper | N/A |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Rabbit anti-ARR3 | Millipore Sigma | Cat#AB15282; RRID:AB_ 1163387 |
| Goat anti-Bhlhb5 | Santa Cruz | Cat#sc-6045; RRID:AB_2065343 |
| Mouse anti-CTBP2 | BD Biosciences | Cat#612044; RRID:AB_399431 |
| Rabbit anti-dsRed | Clontech | Cat#632496; RRID:AB_10013483 |
| Mouse anti-FAT3 | Avilés et al. (2022), Deans et al. (2011) | N/A; RRID:AB_2904260 |
| Chicken anti-GFP | Aves | Cat#GFP-1020; RRID:AB_10000240 |
| Mouse anti-GRIK1 (GluR5) | Santa Cruz Biotechnology | Cat#sc-393420; RRID:AB_2716684 |
| Sheep anti-GRM6 | Cao et al. (2009) | N/A |
| Goat anti-PTPσ | R&D Systems | Cat#AF3430; RRID:AB_2175157 |
| Mouse anti-PTPσ | Medimabs | Cat#MM-0020-P |
| Rabbit anti-VGAT | Synaptic systems | Cat#131002; RRID:AB_887871 |
| Mouse anti-VSX2 (Chx10) | Santa Cruz Biotechnology | Cat#sc-365519; RRID:AB_10842442 |
| Donkey anti-chicken, Alexa Fluor 488 | Jackson ImmunoResearch | Cat#703-545-155; RRID:AB_2340375 |
| Donkey anti-goat, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A11057; RRID:AB_142581 |
| Donkey anti-mouse, Alexa Fluor 488 | Abcam | Cat#ab150105; RRID:AB_2732856 |
| Donkey anti-mouse, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A10037; RRID:AB_2534013 |
| Goat anti-mouse, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A-21235; RRID:AB_2535804 |
| Donkey anti-rabbit, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A21206; RRID:AB_2535792 |
| Donkey anti-rabbit, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A10042; RRID:AB_2534017 |
| Donkey anti-rabbit, Alexa Fluor 647 | Thermo Fisher Scientific | Cat#A31573; RRID:AB_2536183 |
| Donkey anti-sheep, Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A-21099; RRID:AB_2535753 |
| Goat anti-mouse – HRP | BioRad | Cat#170-6516; RRID:AB_11125547 |
| Commercial assays | ||
| RNAscope Multiplex Fluorescent Reagent Kit v2 | ACD | Cat#323100 |
| HCR IHC Bundle | Molecular Instruments | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: Ptf1aCRE | C. Wright, Vanderbilt U (Fujitani et al., 2006) | MGI:2387812 |
| Mouse: Isl1CRE | The Jackson Laboratory (Yang et al., 2006) | Strain #: 024242 |
| Mouse: Bhlhe22CRE | M.E. Greenberg (Harvard Medical School) (Ross et al., 2010) | N/A |
| Mouse: Fat3floxed | Deans et al. (2011) | N/A |
| Mouse: Fat3∆TM | Deans et al. (2011) | N/A |
| Mouse: Fat3∆ICD-GFP | Avilés et al. (2022) | N/A |
| Mouse: Grik1−/− | Christophe Mulle (University of Bordeaux) (Mulle et al., 2000) | N/A |
| Mouse: Grm6−/− | The Jackson Laboratory (Maddox et al., 2008) | Strain #: 016883 |
| Mouse: Ptprs−/− | Michel Tremblay (Elchebly et al., 1999) | MGI:2158757 |
| In situ hybridization probes | ||
| RNAscope Probe- Mm-Fat3-O1 | ACD | Cat#509051 |
| RNAscope Probe- Mm-Grik1-C3 | ACD | Cat#438771-C3 |
| Recombinant DNA | ||
| AAV-Grik1-GFP plasmid | This paper | N/A |
| Viral vectors | ||
| AAV8-Grik1-GFP | This paper | N/A |