Overview of α-Syn and Tau interactomes
| Analyzed protein . | Model . | Method . | Hits . | Enriched pathways . | References . |
|---|---|---|---|---|---|
| α-Syn interactomes | |||||
| α-SynWT | Mouse brain | Co-IP | LRRK2 WT and G2019S | NA | Guerreiro et al. (2013) |
| α-Syn WT and S129A | Yeast | BioID-SILAC with mass spectrometry | 44 | Vesicle transport, endocytosis, membrane organization, actin cytoskeleton, cell cycle, signaling | Popova et al. (2021) |
| α-Syn | Primary rat cortical neurons | Lentivirus transduction of α-Syn–APEX2 | 225 | Vesicle trafficking, calcium, RNA binding, metabolism, actin cytoskeleton, phosphatase, mitophagy | Chung et al. (2017) |
| α-Syn (oligomeric and fibrillar) | Primary rat cortical neurons | Pulldown and mass spectrometry | 32/178 | NA | Shrivastava et al. (2015) |
| α-Syn WT and A53T Tau WT and P301L | Human NGN2-induced synaptophysin+/vesicular glutamate transporter 1+ excitatory neurons | BioID2 | >2,000 | Synapse, plasma membrane, cell cycle, microtubules, RNA, protein containing complex | Griffin et al. (2023) |
| α-SynWT, phosphomimetic S129D, phosphodead S129A | Pulldown with recombinant α-Syn-GST from mouse brain lysate | Co-IP and mass spectrometry | NA | Synaptic proteins, endo- and exocytosis, SV proteins | Parra-Rivas et al. (2023) |
| α-Syn (monomeric and aggregated) | Various human and rodent cell models, patient brains, mouse models | Co-IPs and stainings, no unbiased omics | Rab5, Rab7, Rab11, LAMP1, LAMP2A | Endolysosomal proteins | Hasegawa et al. (2011) and reviewed in Teixeira et al. (2021) |
| Tau interactomes | |||||
| Tau WT, V337M, and P301L | Human NGN2-induced excitatory neurons | APEX with mass spectrometry | 250 | SV, mitochondria, autophagy, proteasome, ribosome | Tracy et al. (2022) |
| TauWT | In vitro interaction between SV and recombinant Tau | Pulldown and mass spectrometry | Syngr3, Syt1, SYNJ1, Rph-3A, Dyn1-3, Sept7 | NA | McInnes et al. (2018) |
| p-Tau | Human sporadic AD brain | Co-IP and mass spectrometry | 125 | Ubiquitin-proteasome, phagosome maturation, necrosis, microtubule associated proteins, 14-3-3 family, kinases, septins | Drummond et al. (2020) |
| Fibrillar Tau | Primary mouse cortical neurons | Pulldown and mass spectrometry | 950/29 membrane protein with extracellular domain | NA | Shrivastava et al. (2019) |
| Analyzed protein . | Model . | Method . | Hits . | Enriched pathways . | References . |
|---|---|---|---|---|---|
| α-Syn interactomes | |||||
| α-SynWT | Mouse brain | Co-IP | LRRK2 WT and G2019S | NA | Guerreiro et al. (2013) |
| α-Syn WT and S129A | Yeast | BioID-SILAC with mass spectrometry | 44 | Vesicle transport, endocytosis, membrane organization, actin cytoskeleton, cell cycle, signaling | Popova et al. (2021) |
| α-Syn | Primary rat cortical neurons | Lentivirus transduction of α-Syn–APEX2 | 225 | Vesicle trafficking, calcium, RNA binding, metabolism, actin cytoskeleton, phosphatase, mitophagy | Chung et al. (2017) |
| α-Syn (oligomeric and fibrillar) | Primary rat cortical neurons | Pulldown and mass spectrometry | 32/178 | NA | Shrivastava et al. (2015) |
| α-Syn WT and A53T Tau WT and P301L | Human NGN2-induced synaptophysin+/vesicular glutamate transporter 1+ excitatory neurons | BioID2 | >2,000 | Synapse, plasma membrane, cell cycle, microtubules, RNA, protein containing complex | Griffin et al. (2023) |
| α-SynWT, phosphomimetic S129D, phosphodead S129A | Pulldown with recombinant α-Syn-GST from mouse brain lysate | Co-IP and mass spectrometry | NA | Synaptic proteins, endo- and exocytosis, SV proteins | Parra-Rivas et al. (2023) |
| α-Syn (monomeric and aggregated) | Various human and rodent cell models, patient brains, mouse models | Co-IPs and stainings, no unbiased omics | Rab5, Rab7, Rab11, LAMP1, LAMP2A | Endolysosomal proteins | Hasegawa et al. (2011) and reviewed in Teixeira et al. (2021) |
| Tau interactomes | |||||
| Tau WT, V337M, and P301L | Human NGN2-induced excitatory neurons | APEX with mass spectrometry | 250 | SV, mitochondria, autophagy, proteasome, ribosome | Tracy et al. (2022) |
| TauWT | In vitro interaction between SV and recombinant Tau | Pulldown and mass spectrometry | Syngr3, Syt1, SYNJ1, Rph-3A, Dyn1-3, Sept7 | NA | McInnes et al. (2018) |
| p-Tau | Human sporadic AD brain | Co-IP and mass spectrometry | 125 | Ubiquitin-proteasome, phagosome maturation, necrosis, microtubule associated proteins, 14-3-3 family, kinases, septins | Drummond et al. (2020) |
| Fibrillar Tau | Primary mouse cortical neurons | Pulldown and mass spectrometry | 950/29 membrane protein with extracellular domain | NA | Shrivastava et al. (2019) |
NA: Not available, SILAC: Stable isotope labeling of amino acids.