Table 1.

List of cell lines

NameTransfectionResistanceCell line number
HeLa EB3-tdTomato-cas9 Retrovirus Puromycin/Hygromycin cER20 
K562 EB3-tdTomato-cas9 Retrovirus Puromycin/Hygromycin cER12 
DLGAP5 iKO Lentivirus Puromycin/Hygromycin cER35 (K562), cER37 (HeLa) 
HMMR iKO Lentivirus Puromycin/Hygromycin cER33 (K562), cER36 (HeLa) 
SAMDH1 iKO Lentivirus Puromycin/Hygromycin cER35 (K562), cER38 (HeLa) 
Kif15 iKO Lentivirus Puromycin/Hygromycin cER13 (HeLa), cER22 (K562) 
HN1 iKO Lentivirus Puromycin/Hygromycin cER17 (HeLa), cER26 (K562) 
HN1L iKO Lentivirus Puromycin/Hygromycin cER16 (HeLa), cER25 (K562) 
HN1/HN1L double knockout Lentivirus Puromycin/Hygromycin cER39 (HeLa) 
CARNMT1 iKO Lentivirus Puromycin/Hygromycin cER15 (HeLa), cER24 (K562) 
NameTransfectionResistanceCell line number
HeLa EB3-tdTomato-cas9 Retrovirus Puromycin/Hygromycin cER20 
K562 EB3-tdTomato-cas9 Retrovirus Puromycin/Hygromycin cER12 
DLGAP5 iKO Lentivirus Puromycin/Hygromycin cER35 (K562), cER37 (HeLa) 
HMMR iKO Lentivirus Puromycin/Hygromycin cER33 (K562), cER36 (HeLa) 
SAMDH1 iKO Lentivirus Puromycin/Hygromycin cER35 (K562), cER38 (HeLa) 
Kif15 iKO Lentivirus Puromycin/Hygromycin cER13 (HeLa), cER22 (K562) 
HN1 iKO Lentivirus Puromycin/Hygromycin cER17 (HeLa), cER26 (K562) 
HN1L iKO Lentivirus Puromycin/Hygromycin cER16 (HeLa), cER25 (K562) 
HN1/HN1L double knockout Lentivirus Puromycin/Hygromycin cER39 (HeLa) 
CARNMT1 iKO Lentivirus Puromycin/Hygromycin cER15 (HeLa), cER24 (K562) 

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