Summary of patches/events for different human NMDAR subunits
| Construct . | Internal soln . | pH . | Interval (s) . | Total events (# of patches) . | Average latency (ms) . | Successes % (global) . | Efficiency (tcrit) . |
|---|---|---|---|---|---|---|---|
| rN1/rN2A | ATP | 7.4 | 10 | 1,407 (21) | 42 ± 7 | 83 ± 2 (85) | 0.78 ± 0.02 (69.8) |
| hN1/hN2A | “ | “ | “ | 469 (16) | 23 ± 5 | 87 ± 2 (88) | 0.85 ± 0.03 (70.1) |
| hN1/hN2B | “ | “ | 20 | 1,234 (25) | 290 ± 50 | 76 ± 3 (76) | 0.56 ± 0.04 (148) |
| hN1/hN2C | “ | “ | 10 | 660 (18) | 76 ± 9 | 97 ± 1 (97) | 0.92 ± 0.02 (284) |
| hN1/hN2D | “ | “ | “ | 486 (10) | 74 ± 15 | 98 ± 1 (98) | 0.92 ± 0.02 (201) |
| Construct . | Internal soln . | pH . | Interval (s) . | Total events (# of patches) . | Average latency (ms) . | Successes % (global) . | Efficiency (tcrit) . |
|---|---|---|---|---|---|---|---|
| rN1/rN2A | ATP | 7.4 | 10 | 1,407 (21) | 42 ± 7 | 83 ± 2 (85) | 0.78 ± 0.02 (69.8) |
| hN1/hN2A | “ | “ | “ | 469 (16) | 23 ± 5 | 87 ± 2 (88) | 0.85 ± 0.03 (70.1) |
| hN1/hN2B | “ | “ | 20 | 1,234 (25) | 290 ± 50 | 76 ± 3 (76) | 0.56 ± 0.04 (148) |
| hN1/hN2C | “ | “ | 10 | 660 (18) | 76 ± 9 | 97 ± 1 (97) | 0.92 ± 0.02 (284) |
| hN1/hN2D | “ | “ | “ | 486 (10) | 74 ± 15 | 98 ± 1 (98) | 0.92 ± 0.02 (201) |
Values are displayed as in Table 1. For all recordings, our internal solution was energy-based (ATP and GTP), the external solution was our standard external solution at pH 7.4; and there was a 10- or 20-s delay between applications. Constructs are either rat (r) or human (h). In all instances, the GluN1 subunit was the GluN1-1a splice variant.