Summary of patches/events under different physiological conditions
| Construct . | Internal soln . | pH . | Interval (s) . | Total events (total # of patches) . | Average latency (ms) . | Successes % (global) . | Efficiency (tcrit) . |
|---|---|---|---|---|---|---|---|
| rN1-1a/rN2A | No ATP, GTP | 8 | 4 | 2,053 (22) | 47 ± 7 | 80 ± 2 (74) | 0.72 ± 0.02 (42.7) |
| ATP and GTP | 8 | 4 | 559 (6) | 25 ± 8 | 93 ± 3 (91) | 0.90 ± 0.04 (50.5) | |
| No ATP, GTP | 8 | 10 | 789 (14) | 22 ± 7 | 88 ± 3 (90) | 0.86 ± 0.04 (59.7) | |
| ATP and GTP | 8 | 10 | 583 (12) | 23 ± 6 | 93 ± 2 (91) | 0.90 ± 0.02 (52.9) | |
| ATP and GTP | 7.4 | 10 | 1,407 (21) | 42 ± 7 | 83 ± 2 (85) | 0.78 ± 0.02 (70.1) |
| Construct . | Internal soln . | pH . | Interval (s) . | Total events (total # of patches) . | Average latency (ms) . | Successes % (global) . | Efficiency (tcrit) . |
|---|---|---|---|---|---|---|---|
| rN1-1a/rN2A | No ATP, GTP | 8 | 4 | 2,053 (22) | 47 ± 7 | 80 ± 2 (74) | 0.72 ± 0.02 (42.7) |
| ATP and GTP | 8 | 4 | 559 (6) | 25 ± 8 | 93 ± 3 (91) | 0.90 ± 0.04 (50.5) | |
| No ATP, GTP | 8 | 10 | 789 (14) | 22 ± 7 | 88 ± 3 (90) | 0.86 ± 0.04 (59.7) | |
| ATP and GTP | 8 | 10 | 583 (12) | 23 ± 6 | 93 ± 2 (91) | 0.90 ± 0.02 (52.9) | |
| ATP and GTP | 7.4 | 10 | 1,407 (21) | 42 ± 7 | 83 ± 2 (85) | 0.78 ± 0.02 (70.1) |
Values shown are mean ± SEM for average latency of the delay to first opening, Successes (average of successes for individual patches), and Efficiency (= [successes − slow component]/total trial number); see Materials and methods). The number below the total events shows the total number of patches recorded (a patch required a minimum of 10 trials to be included). Global successes (total number successes/total number of trials) and tcrit are indicated in the last two columns. Recordings were made under different conditions: an internal solution either containing no ATP and GTP (energy-free) or one containing these elements (energy-based); our standard external solution at pH 8 or 7.4; and/or a delay between the start of glutamate applications of 4 or 10 s. All constructs are rats. Single channels were recorded in the outside-out mode at −60 mV (e.g., Fig. 1) and analyzed in Igor Pro (see Materials and methods).