List of monoclonal antibodies and live/dead cellular dyes used
| Specificity . | Fluorochrome . | Clone . | Company/Source . |
|---|---|---|---|
| Used in mouse experiments | |||
| CD16/32 | N/A | 2.4G2 | Produced in-house |
| 7-AAD | N/A | N/A | Sigma-Aldrich |
| Zombie NIR fixable viability dye | N/A | N/A | BioLegend |
| Annexin V | BV711 | N/A | BD Horizon |
| Annexin V | FITC | N/A | BD Pharmingen |
| ARTC2 | APC | Nika102 | Novus Biologicals |
| B220 (CD45R) | BUV496 | RA3-6B2 | BD Horizon |
| B220 (CD45R) | BV786 | RA3-6B2 | BD Horizon |
| CD3 | BUV395 | 145-2C11 | BD Horizon |
| CD4 | BUV395 | GK1.5 | BD Horizon |
| CD4 | AF532 | RM4-5 | Invitrogen |
| CD8α | BUV805 | 53-6.7 | BD Horizon |
| CD27 | BUV737 | LG.3A10 | BD Horizon |
| CD27 | BV605 | LG.3A10 | BD Horizon |
| CD38 | FITC | 90/CD38 | BD Pharmingen |
| CD38 | BV711 | 90/CD38 | BD OptiBuild |
| CD44 | AF700 | IM7 | Invitrogen |
| CD45.1 | PE-Cy7 | A20 | BD Pharmingen |
| CD62L | APC | MEL-14 | Invitrogen |
| CD62L | PerCP-Cy5.5 | MEL-14 | Invitrogen |
| CD69 | BUV737 | H1.2F3 | BD Horizon |
| CD319 | APC | 4G2 | BioLegend |
| ICOS (CD278) | BV711 | C398.4A | BioLegend |
| IFN-γ | PE-Cy7 | XMG1.2 | BioLegend |
| IFN-γ | FITC | XMG1.2 | BD Pharmingen |
| IL-4 | AF647 | 11B11 | BD Pharmingen |
| IL-17A | AF647 | TC11-18H10 | BD Pharmingen |
| IL-17A | PerCP-Cy5.5 | TC11-18H10 | BD Pharmingen |
| P2RX7 | PE | 1F11 | BioLegend |
| P2RX7 | PE-Cy7 | 1F11 | BioLegend |
| PLZF | PE-Cy7 | 9E12 | BioLegend |
| RORγt | BV786 | Q31-378 | BD Horizon |
| RORγt | PerCP-Cy5.5 | Q31-378 | BD Pharmingen |
| T-bet | AF488 | 4B10 | BioLegend |
| T-bet | BV711 | 4B10 | BioLegend |
| TCRβ | APC-Cy7 | H57-597 | BD Pharmingen |
| γδTCR | BV605 | GL3 | BioLegend |
| γδTCR | BV605 | GL3 | BD OptiBuild |
| γδTCR | FITC | GL3 | BD Pharmingen |
| Used in human experiments | |||
| 7-AAD | N/A | N/A | Sigma-Aldrich |
| LIVE/DEAD blue Near-IR | N/A | N/A | Invitrogen |
| LIVE/DEAD fixable near-IR | N/A | N/A | Invitrogen |
| Zombie NIR fixable viability dye | N/A | N/A | BioLegend |
| CD3 | AF700 | UCHT2 | BD Pharmingen |
| CD3 | BUV395 | UCHT2 | BD Horizon |
| CD4 | BUV496 | SK3 | BD Horizon |
| CD4 | AF532 | RM4-5 | Invitrogen |
| CD8α | BUV805 | SK1 | BD Pharmingen |
| CD14 | BUV395 | MφP9 | BD Pharmingen |
| CD14 | APC-Cy7 | MφP9 | BD Pharmingen |
| CD14 | BV570 | M5E2 | BioLegend |
| CD19 | APC-Cy7 | SJ25C1 | BD Pharmingen |
| CD19 | BV570 | HIB19 | BD Pharmingen |
| CD19 | BUV737 | SJ25C1 | BioLegend |
| CD27 | BV785 | O323 | BioLegend |
| CD45 | AF700 | 2D1 | BioLegend |
| CD62L | FITC | DREG-56 | BD Pharmingen |
| CD161 | PerCP-Cy5.5 | HP-3G10 | BioLegend |
| CD161 | PE-Cy7 | HP-3G10 | BioLegend |
| FcR blocking reagent | N/A | N/A | Miltenyi Biotec |
| P2RX7 | AF647 | L4 | (Buell et al., 1998; Elhage et al., 2022) Provided by Dr. Xin Huang and Dr. Ben Gu, the University of Melbourne, Florey Institute of Neuroscience and Mental Health |
| Vα7.2 | BV711 | 3C10 | BioLegend |
| Vα7.2 | BV605 | 3C10 | BioLegend |
| Vδ1 | FITC | TS8.2 | Invitrogen |
| Vδ1 | VioBlue | REA173 | Miltenyi Biotec |
| Vδ2 | BV711 | B6 | BioLegend |
| Vδ2 | BUV563 | B6 | BD OptiBuild |
| γδTCR | PE-Cy7 | 11F2 | BD |
| γδTCR | R718 | 11F2 | BD OptiBuild |
| Specificity . | Fluorochrome . | Clone . | Company/Source . |
|---|---|---|---|
| Used in mouse experiments | |||
| CD16/32 | N/A | 2.4G2 | Produced in-house |
| 7-AAD | N/A | N/A | Sigma-Aldrich |
| Zombie NIR fixable viability dye | N/A | N/A | BioLegend |
| Annexin V | BV711 | N/A | BD Horizon |
| Annexin V | FITC | N/A | BD Pharmingen |
| ARTC2 | APC | Nika102 | Novus Biologicals |
| B220 (CD45R) | BUV496 | RA3-6B2 | BD Horizon |
| B220 (CD45R) | BV786 | RA3-6B2 | BD Horizon |
| CD3 | BUV395 | 145-2C11 | BD Horizon |
| CD4 | BUV395 | GK1.5 | BD Horizon |
| CD4 | AF532 | RM4-5 | Invitrogen |
| CD8α | BUV805 | 53-6.7 | BD Horizon |
| CD27 | BUV737 | LG.3A10 | BD Horizon |
| CD27 | BV605 | LG.3A10 | BD Horizon |
| CD38 | FITC | 90/CD38 | BD Pharmingen |
| CD38 | BV711 | 90/CD38 | BD OptiBuild |
| CD44 | AF700 | IM7 | Invitrogen |
| CD45.1 | PE-Cy7 | A20 | BD Pharmingen |
| CD62L | APC | MEL-14 | Invitrogen |
| CD62L | PerCP-Cy5.5 | MEL-14 | Invitrogen |
| CD69 | BUV737 | H1.2F3 | BD Horizon |
| CD319 | APC | 4G2 | BioLegend |
| ICOS (CD278) | BV711 | C398.4A | BioLegend |
| IFN-γ | PE-Cy7 | XMG1.2 | BioLegend |
| IFN-γ | FITC | XMG1.2 | BD Pharmingen |
| IL-4 | AF647 | 11B11 | BD Pharmingen |
| IL-17A | AF647 | TC11-18H10 | BD Pharmingen |
| IL-17A | PerCP-Cy5.5 | TC11-18H10 | BD Pharmingen |
| P2RX7 | PE | 1F11 | BioLegend |
| P2RX7 | PE-Cy7 | 1F11 | BioLegend |
| PLZF | PE-Cy7 | 9E12 | BioLegend |
| RORγt | BV786 | Q31-378 | BD Horizon |
| RORγt | PerCP-Cy5.5 | Q31-378 | BD Pharmingen |
| T-bet | AF488 | 4B10 | BioLegend |
| T-bet | BV711 | 4B10 | BioLegend |
| TCRβ | APC-Cy7 | H57-597 | BD Pharmingen |
| γδTCR | BV605 | GL3 | BioLegend |
| γδTCR | BV605 | GL3 | BD OptiBuild |
| γδTCR | FITC | GL3 | BD Pharmingen |
| Used in human experiments | |||
| 7-AAD | N/A | N/A | Sigma-Aldrich |
| LIVE/DEAD blue Near-IR | N/A | N/A | Invitrogen |
| LIVE/DEAD fixable near-IR | N/A | N/A | Invitrogen |
| Zombie NIR fixable viability dye | N/A | N/A | BioLegend |
| CD3 | AF700 | UCHT2 | BD Pharmingen |
| CD3 | BUV395 | UCHT2 | BD Horizon |
| CD4 | BUV496 | SK3 | BD Horizon |
| CD4 | AF532 | RM4-5 | Invitrogen |
| CD8α | BUV805 | SK1 | BD Pharmingen |
| CD14 | BUV395 | MφP9 | BD Pharmingen |
| CD14 | APC-Cy7 | MφP9 | BD Pharmingen |
| CD14 | BV570 | M5E2 | BioLegend |
| CD19 | APC-Cy7 | SJ25C1 | BD Pharmingen |
| CD19 | BV570 | HIB19 | BD Pharmingen |
| CD19 | BUV737 | SJ25C1 | BioLegend |
| CD27 | BV785 | O323 | BioLegend |
| CD45 | AF700 | 2D1 | BioLegend |
| CD62L | FITC | DREG-56 | BD Pharmingen |
| CD161 | PerCP-Cy5.5 | HP-3G10 | BioLegend |
| CD161 | PE-Cy7 | HP-3G10 | BioLegend |
| FcR blocking reagent | N/A | N/A | Miltenyi Biotec |
| P2RX7 | AF647 | L4 | (Buell et al., 1998; Elhage et al., 2022) Provided by Dr. Xin Huang and Dr. Ben Gu, the University of Melbourne, Florey Institute of Neuroscience and Mental Health |
| Vα7.2 | BV711 | 3C10 | BioLegend |
| Vα7.2 | BV605 | 3C10 | BioLegend |
| Vδ1 | FITC | TS8.2 | Invitrogen |
| Vδ1 | VioBlue | REA173 | Miltenyi Biotec |
| Vδ2 | BV711 | B6 | BioLegend |
| Vδ2 | BUV563 | B6 | BD OptiBuild |
| γδTCR | PE-Cy7 | 11F2 | BD |
| γδTCR | R718 | 11F2 | BD OptiBuild |