Reagents and resources
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Dulbecco’s phosphate buffered saline (1×) | Sigma-Aldrich | BSS-1005-B |
| Dulbecco’s phosphate buffered saline (10×) | Sigma-Aldrich | 6506-OP |
| Dulbecco’s phosphate buffered saline (1×) w/o CA+ & Mg++ | Sigma-Aldrich | BSS-1006 |
| Hanks’ balanced salt solution w/ CA++ & Mg++ (1×) | GIBCO | 14175095 |
| Bovine serum albumin | Millipore sigma | B6917 |
| GlutaMAX | GIBCO | 35050061 |
| Sodium pyruvate | GIBCO | 11360070 |
| MEM non-essential amino acids solution | GIBCO | 11140050 |
| 2-Mercaptoethanol | Sigma-Aldrich | M6250 |
| Red cell lysis buffer | Biolegend | 420301 |
| Percoll | Sigma-Aldrich | P1644 |
| Dulbecco’s modified Eagle medium | Gibco | 11995-065 |
| RPMI-1640 | Sigma-Aldrich | R8758-500 ML |
| DNAse | Sigma-Aldrich | DN-25 |
| Collagenase | Sigma-Aldrich | C5138-1G |
| EDTA pH 8.0, 0.5M | BioShop | EDT111.500 |
| HEPES | Gibco | 15630-080 |
| Fetal bovine serum | Gibco | 26140079 |
| Foxp3 transcription factor staining buffer set | eBioscience | 00-5523-00 |
| DNAZap | Invitrogen | AM9890 |
| Fischerbrand superfrost plus microscope slides | Fischerscientific | 12-550-15 |
| TritonX | Sigma-Aldrich | T9284 |
| Streptomycin sulfate salt | Sigma-Aldrich | S6501 |
| Penicillin-streptomycin | Sigma-Aldrich | P4333 |
| Fluoroshield mounting media | Sigma-Aldrich | F6182 |
| Fluoroshield mounting media with DAPI | Sigma-Aldrich | F6057 |
| Microvette CB 300Z blood collection capillary tube | Sarstedt | 16.440.100 |
| Nunc MaxiSorp ELISA plates, uncoated | BioLegend | 423501 |
| Mouse Anti-OVA IgA antibody assay kit | Chondrex | 3018 |
| TMB substrate | Biolegend | 422101 |
| Sulfuric acid (5.0N Solution) | Bioshop | SUL500.1 |
| Tween20 | Bioshop | TWN510.500 |
| UltraPure 1M Tric-HCL pH 7.5 | Invitrogen | 15567-027 |
| Ovalumin grade V | Millipore sigma | A5503 |
| Zirconium beads | BioSpec | 11079101z |
| QIAquick qPCR purification kit | QIAGEN | 28104 |
| TRI reagent (Trizol) | Sigma-Aldrich | T9424 |
| RNeasy mini kit | QIAGEN | 74106 |
| High-capacity RNA-to-cDNA | Applied Biosystems | 4387406 |
| QX200 ddPCR EvaGreen supermix | Bio-Rad | 1864034 |
| QX200 droplet Generation oil for EvaGreen | Bio-Rad | 1864005 |
| PowerTrack SYBR green MM | ThermoFisher | A46012 |
| QIAamp DNA microbiome kit | QIAGEN | 51704 |
| Streptomycin sulfate salt | Sigma-Aldrich | S6501-50 G |
| Phenol-chloroform | Ambion | AM9732 |
| Chloroform | Caledon | 3000-1-10 |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Dulbecco’s phosphate buffered saline (1×) | Sigma-Aldrich | BSS-1005-B |
| Dulbecco’s phosphate buffered saline (10×) | Sigma-Aldrich | 6506-OP |
| Dulbecco’s phosphate buffered saline (1×) w/o CA+ & Mg++ | Sigma-Aldrich | BSS-1006 |
| Hanks’ balanced salt solution w/ CA++ & Mg++ (1×) | GIBCO | 14175095 |
| Bovine serum albumin | Millipore sigma | B6917 |
| GlutaMAX | GIBCO | 35050061 |
| Sodium pyruvate | GIBCO | 11360070 |
| MEM non-essential amino acids solution | GIBCO | 11140050 |
| 2-Mercaptoethanol | Sigma-Aldrich | M6250 |
| Red cell lysis buffer | Biolegend | 420301 |
| Percoll | Sigma-Aldrich | P1644 |
| Dulbecco’s modified Eagle medium | Gibco | 11995-065 |
| RPMI-1640 | Sigma-Aldrich | R8758-500 ML |
| DNAse | Sigma-Aldrich | DN-25 |
| Collagenase | Sigma-Aldrich | C5138-1G |
| EDTA pH 8.0, 0.5M | BioShop | EDT111.500 |
| HEPES | Gibco | 15630-080 |
| Fetal bovine serum | Gibco | 26140079 |
| Foxp3 transcription factor staining buffer set | eBioscience | 00-5523-00 |
| DNAZap | Invitrogen | AM9890 |
| Fischerbrand superfrost plus microscope slides | Fischerscientific | 12-550-15 |
| TritonX | Sigma-Aldrich | T9284 |
| Streptomycin sulfate salt | Sigma-Aldrich | S6501 |
| Penicillin-streptomycin | Sigma-Aldrich | P4333 |
| Fluoroshield mounting media | Sigma-Aldrich | F6182 |
| Fluoroshield mounting media with DAPI | Sigma-Aldrich | F6057 |
| Microvette CB 300Z blood collection capillary tube | Sarstedt | 16.440.100 |
| Nunc MaxiSorp ELISA plates, uncoated | BioLegend | 423501 |
| Mouse Anti-OVA IgA antibody assay kit | Chondrex | 3018 |
| TMB substrate | Biolegend | 422101 |
| Sulfuric acid (5.0N Solution) | Bioshop | SUL500.1 |
| Tween20 | Bioshop | TWN510.500 |
| UltraPure 1M Tric-HCL pH 7.5 | Invitrogen | 15567-027 |
| Ovalumin grade V | Millipore sigma | A5503 |
| Zirconium beads | BioSpec | 11079101z |
| QIAquick qPCR purification kit | QIAGEN | 28104 |
| TRI reagent (Trizol) | Sigma-Aldrich | T9424 |
| RNeasy mini kit | QIAGEN | 74106 |
| High-capacity RNA-to-cDNA | Applied Biosystems | 4387406 |
| QX200 ddPCR EvaGreen supermix | Bio-Rad | 1864034 |
| QX200 droplet Generation oil for EvaGreen | Bio-Rad | 1864005 |
| PowerTrack SYBR green MM | ThermoFisher | A46012 |
| QIAamp DNA microbiome kit | QIAGEN | 51704 |
| Streptomycin sulfate salt | Sigma-Aldrich | S6501-50 G |
| Phenol-chloroform | Ambion | AM9732 |
| Chloroform | Caledon | 3000-1-10 |