Table 1.

Antibodies

FluorophoreAntibodyCloneCompanyReference #
APC Rat anti-mouse B220 (CD45R) RA3-6B2 BioLegend 103212 
APC Rat anti-mouse PD-1 (CD279) 29F.1A12 BioLegend 135210 
AF647 Goat anti-mouse IgA N/A Southern Biotech 1040-31 
eF450 Rat anti-mouse CD3 17A2 eBioscience 48-0032-82 
eF450 Rat anti-mouse MHC-II (I-A/I-E) M5/114.15.2 BioLegend 107607 
FITC Rat anti-mouse CD40L (CD154) SA047C3 BioLegend 157005 
FITC Rat anti-mouse CD4 GK1.5 BioLegend 100406 
FITC Rat anti-mouse CD40L (CD154) SA047C3 BioLegend 157006 
FITC Anti-mouse FAS (CD95) SA367H8 BioLegend 152606 
FITC Goat anti-mouse IgA N/A Southern Biotech 1165-02 
FITC Rat anti-mouse Ki-67 16A8 BioLegend 652410 
FITC Rat anti-mouse/rat XCR1 ZET BioLegend 148210 
FITC SYTOBC green fluorescent nucleic acid stain N/A ThermoFisher S34855 
APC Rat anti-mouse B220 RA3-6B2 BioLegend 103231 
APC Rat anti-mouse PD-1 RMP1-30 BioLegend 109111 
APC Rat anti-mouse CD40 3/23 BioLegend 124612 
APC Cy7 Anti-mouse CD45.2 104 BioLegend 109824 
PE Rat anti-mouse CD197 (CCR7) 4B12 BioLegend 120106 
PE Rat anti-mouse CD138 281-2 BioLegend 142504 
PE Hamster anti-mouse CD154 MR1 BioLegend 106505 
PE Rat anti-mouse GL-7 GL7 BioLegend 144608 
PE Rat anti-mouse Foxp3 MF-14 BioLegend 126404 
PE Rat anti-mouse CCR7 4B12 BioLegend 120106 
PE Rat anti-mouse ICOSL HK5.3 BioLegend 107405 
PE Cy7 Anti-mouse/human CD11b M1/70 BioLegend 101216 
PE Cy7 Hamster anti-mouse ICOS C398.4A BioLegend 313520 
PE Cy7 Rat anti-mouse CD4 GK1.5 BioLegend 100422 
PerCP Cy5.5 Rat anti-mouse IgM RMM-1 BioLegend 406512 
PerCP e710 Rat anti-mouse CXCR5 SPRCL5 eBioscience 46-7185-82 
BV 605 Rat anti-mouse B220 RA3-6B2 BioLegend 103243 
eFluor 506 Fixable viability N/A eBioscience 65-0866-14 
HRP Goat anti-mouse IgA N/A Southern Biotech 1040-05 
N/A InVivoMAb anti-mouse ICOSL HK5.3, BioXCell BE0028 
N/A InVivoMAb anti-mouse CD4 GK1.5 BioXCell BE0003-1 
FluorophoreAntibodyCloneCompanyReference #
APC Rat anti-mouse B220 (CD45R) RA3-6B2 BioLegend 103212 
APC Rat anti-mouse PD-1 (CD279) 29F.1A12 BioLegend 135210 
AF647 Goat anti-mouse IgA N/A Southern Biotech 1040-31 
eF450 Rat anti-mouse CD3 17A2 eBioscience 48-0032-82 
eF450 Rat anti-mouse MHC-II (I-A/I-E) M5/114.15.2 BioLegend 107607 
FITC Rat anti-mouse CD40L (CD154) SA047C3 BioLegend 157005 
FITC Rat anti-mouse CD4 GK1.5 BioLegend 100406 
FITC Rat anti-mouse CD40L (CD154) SA047C3 BioLegend 157006 
FITC Anti-mouse FAS (CD95) SA367H8 BioLegend 152606 
FITC Goat anti-mouse IgA N/A Southern Biotech 1165-02 
FITC Rat anti-mouse Ki-67 16A8 BioLegend 652410 
FITC Rat anti-mouse/rat XCR1 ZET BioLegend 148210 
FITC SYTOBC green fluorescent nucleic acid stain N/A ThermoFisher S34855 
APC Rat anti-mouse B220 RA3-6B2 BioLegend 103231 
APC Rat anti-mouse PD-1 RMP1-30 BioLegend 109111 
APC Rat anti-mouse CD40 3/23 BioLegend 124612 
APC Cy7 Anti-mouse CD45.2 104 BioLegend 109824 
PE Rat anti-mouse CD197 (CCR7) 4B12 BioLegend 120106 
PE Rat anti-mouse CD138 281-2 BioLegend 142504 
PE Hamster anti-mouse CD154 MR1 BioLegend 106505 
PE Rat anti-mouse GL-7 GL7 BioLegend 144608 
PE Rat anti-mouse Foxp3 MF-14 BioLegend 126404 
PE Rat anti-mouse CCR7 4B12 BioLegend 120106 
PE Rat anti-mouse ICOSL HK5.3 BioLegend 107405 
PE Cy7 Anti-mouse/human CD11b M1/70 BioLegend 101216 
PE Cy7 Hamster anti-mouse ICOS C398.4A BioLegend 313520 
PE Cy7 Rat anti-mouse CD4 GK1.5 BioLegend 100422 
PerCP Cy5.5 Rat anti-mouse IgM RMM-1 BioLegend 406512 
PerCP e710 Rat anti-mouse CXCR5 SPRCL5 eBioscience 46-7185-82 
BV 605 Rat anti-mouse B220 RA3-6B2 BioLegend 103243 
eFluor 506 Fixable viability N/A eBioscience 65-0866-14 
HRP Goat anti-mouse IgA N/A Southern Biotech 1040-05 
N/A InVivoMAb anti-mouse ICOSL HK5.3, BioXCell BE0028 
N/A InVivoMAb anti-mouse CD4 GK1.5 BioXCell BE0003-1 

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