Antibodies and reagents for flow cytometry and immunofluorescence microscopy
| Antibody target or reagent . | Fluorochrome . | Clone . | Supplier . | Reference . |
|---|---|---|---|---|
| Perilipin-2/ADFP | Uncoupled | EPR3713 | Abcam | ab108323 |
| Phospho-AMPKα (T183/T172) | Uncoupled | Polyclonal | Abcam | ab23875 |
| SQSTM1/p62 | Uncoupled | 2C11 | Abcam | ab56416 |
| Active Caspase-3 | FITC | C92-605 | BD Biosciences | 559341 |
| BrdU | FITC | B44 | BD Biosciences | 552598 |
| CD103 | PE | M290 | BD Biosciences | 557495 |
| CD11c | PerCP-Cy5.5 | HL3 | BD Biosciences | 560584 |
| CD36 | PE | CRF D-2712 | BD Biosciences | 562702 |
| CD3ε | FITC | 145-2C11 | BD Biosciences | 553062 |
| CD71 | FITC | C2 | BD Biosciences | 553266 |
| CD8α | APC | 53-6.7 | BD Biosciences | 561093 |
| Gr-1 | PE | RB6-8C5 | BD Biosciences | 553128 |
| I-A/I-E | Biotinylated | M5114.15.2 | BD Biosciences | 553622 |
| Ki67 | PerCP-Cy5.5 | B56 | BD Biosciences | 561284 |
| Ly-6G | FITC | 1A8 | BD Biosciences | 551460 |
| β3-tubulin | Uncoupled | TUJ1 | Biolegend | 801202 |
| CD11b | PerCP-Cy5.5 | M1/70 | Biolegend | 101228 |
| CD3ε | APC | 145-2C11 | Biolegend | 100312 |
| CD3ε | PerCP-Cy5.5 | 145-2C11 | Biolegend | 100328 |
| CD45 | PE-Cy7 | 30F11 | Biolegend | 103114 |
| CD45 | APC-Cy7 | 30F11 | Biolegend | 103116 |
| CD86 | PE | GL-1 | Biolegend | 105008 |
| I-A/I-E | AlexaFluor 700 | M5114.15.2 | Biolegend | 107622 |
| TCRγδd | PE | GL3 | Biolegend | 118107 |
| Fixable viability dye | eFluor450 | N/A | eBioscience | 65-0863-14 |
| Fixable viability dye | eFluor780 | N/A | eBioscience | 65-0865-14 |
| I-A/I-E | PE | M5114.15.1 | eBioscience | 12-5321-81 |
| TCRγδ | APC | GL3 | eBioscience | 17-5711-82 |
| CD207 | AlexaFluor 647 | 929F3 | Eurobio/Dendritics | DDX0362A647 |
| CD207 | AlexaFluor 488 | 929F3 | Eurobio/Dendritics | DDX0362A488 |
| Bodipy | Bodipy 493/503 | N/A | Invitrogen | D3922 |
| Bodipy C11 | Bodipy 581/591 | N/A | Invitrogen | D3861 |
| Bodipy FL C16 | Bodipy 505/512 | N/A | Invitrogen | D3821 |
| DAPI | N/A | N/A | Invitrogen | D3571 |
| Donkey anti Rabbit IgG | AlexaFluor 647 | Polyclonal | Invitrogen | A31573 |
| ER tracker Blue/White DPX | N/A | N/A | Invitrogen | E12353 |
| Lysosensor green DND-189 | N/A | N/A | Invitrogen | L7535 |
| Lysotracker red DND-99 | N/A | N/A | Invitrogen | L7528 |
| Mitosox red | N/A | N/A | Invitrogen | M36008 |
| Mitotracker deep red 633 | N/A | N/A | Invitrogen | M22426 |
| Mitotracker green FM | N/A | N/A | Invitrogen | M7514 |
| Mouse IgG(H+L) | AlexaFluor 555 | Polyclonal | Invitrogen | A31570 |
| Mouse IgG(H+L) | AlexaFluor 594 | Polyclonal | Invitrogen | A11032 |
| Streptavidin | AlexaFluor 488 | N/A | Invitrogen | S11223 |
| Streptavidin | AlexaFluor 546 | N/A | Invitrogen | S11225 |
| Poly (I:C) | N/A | N/A | InvivoGen | tlrl-pic |
| Guava autophagy LC3 assay kit | FITC | 4E12 | Luminex | FCCH100171 |
| CD11b-coupled Microbeads | N/A | N/A | Miltenyi Biotec | 130-049-601 |
| 2-NBDG | N/A | N/A | Sigma-Aldrich | 72987 |
| Aluminium hydroxide | N/A | N/A | Sigma-Aldrich | 239186 |
| Etomoxir | N/A | N/A | Sigma-Aldrich | E1905 |
| Ferrostatin-1 | N/A | N/A | Sigma-Aldrich | SML0583 |
| Wortmannin | N/A | N/A | Sigma-Aldrich | W1628 |
| Fast SYBR green master mix | N/A | N/A | Thermo Fisher Scientific | 4385612 |
| RevertAid H minus first strand cDNA synthesis kit | N/A | N/A | Thermo Fisher Scientific | K1632 |
| GLUT1 | Uncoupled | EPR3915 | Abcam | ab115730 |
| BioTracker FerroOrange | N/A | N/A | Sigma-Aldrich | SCT210 |
| BD Phosflow fix buffer I | N/A | N/A | BD Biosciences | 557870 |
| BD Phosflow Perm buffer III | N/A | N/A | BD Biosciences | 558050 |
| Phospho-4E-BP1 (pT36/pT45) | AlexaFluor 488 | M31-16 | BD Biosciences | 560287 |
| Phospho-S6 (pS244) | PE | N5-676 | BD Biosciences | 560462 |
| Phospho-Akt (pS473) | Brilliant Violet 421 | M89-61 | BD Biosciences | 562599 |
| Antibody target or reagent . | Fluorochrome . | Clone . | Supplier . | Reference . |
|---|---|---|---|---|
| Perilipin-2/ADFP | Uncoupled | EPR3713 | Abcam | ab108323 |
| Phospho-AMPKα (T183/T172) | Uncoupled | Polyclonal | Abcam | ab23875 |
| SQSTM1/p62 | Uncoupled | 2C11 | Abcam | ab56416 |
| Active Caspase-3 | FITC | C92-605 | BD Biosciences | 559341 |
| BrdU | FITC | B44 | BD Biosciences | 552598 |
| CD103 | PE | M290 | BD Biosciences | 557495 |
| CD11c | PerCP-Cy5.5 | HL3 | BD Biosciences | 560584 |
| CD36 | PE | CRF D-2712 | BD Biosciences | 562702 |
| CD3ε | FITC | 145-2C11 | BD Biosciences | 553062 |
| CD71 | FITC | C2 | BD Biosciences | 553266 |
| CD8α | APC | 53-6.7 | BD Biosciences | 561093 |
| Gr-1 | PE | RB6-8C5 | BD Biosciences | 553128 |
| I-A/I-E | Biotinylated | M5114.15.2 | BD Biosciences | 553622 |
| Ki67 | PerCP-Cy5.5 | B56 | BD Biosciences | 561284 |
| Ly-6G | FITC | 1A8 | BD Biosciences | 551460 |
| β3-tubulin | Uncoupled | TUJ1 | Biolegend | 801202 |
| CD11b | PerCP-Cy5.5 | M1/70 | Biolegend | 101228 |
| CD3ε | APC | 145-2C11 | Biolegend | 100312 |
| CD3ε | PerCP-Cy5.5 | 145-2C11 | Biolegend | 100328 |
| CD45 | PE-Cy7 | 30F11 | Biolegend | 103114 |
| CD45 | APC-Cy7 | 30F11 | Biolegend | 103116 |
| CD86 | PE | GL-1 | Biolegend | 105008 |
| I-A/I-E | AlexaFluor 700 | M5114.15.2 | Biolegend | 107622 |
| TCRγδd | PE | GL3 | Biolegend | 118107 |
| Fixable viability dye | eFluor450 | N/A | eBioscience | 65-0863-14 |
| Fixable viability dye | eFluor780 | N/A | eBioscience | 65-0865-14 |
| I-A/I-E | PE | M5114.15.1 | eBioscience | 12-5321-81 |
| TCRγδ | APC | GL3 | eBioscience | 17-5711-82 |
| CD207 | AlexaFluor 647 | 929F3 | Eurobio/Dendritics | DDX0362A647 |
| CD207 | AlexaFluor 488 | 929F3 | Eurobio/Dendritics | DDX0362A488 |
| Bodipy | Bodipy 493/503 | N/A | Invitrogen | D3922 |
| Bodipy C11 | Bodipy 581/591 | N/A | Invitrogen | D3861 |
| Bodipy FL C16 | Bodipy 505/512 | N/A | Invitrogen | D3821 |
| DAPI | N/A | N/A | Invitrogen | D3571 |
| Donkey anti Rabbit IgG | AlexaFluor 647 | Polyclonal | Invitrogen | A31573 |
| ER tracker Blue/White DPX | N/A | N/A | Invitrogen | E12353 |
| Lysosensor green DND-189 | N/A | N/A | Invitrogen | L7535 |
| Lysotracker red DND-99 | N/A | N/A | Invitrogen | L7528 |
| Mitosox red | N/A | N/A | Invitrogen | M36008 |
| Mitotracker deep red 633 | N/A | N/A | Invitrogen | M22426 |
| Mitotracker green FM | N/A | N/A | Invitrogen | M7514 |
| Mouse IgG(H+L) | AlexaFluor 555 | Polyclonal | Invitrogen | A31570 |
| Mouse IgG(H+L) | AlexaFluor 594 | Polyclonal | Invitrogen | A11032 |
| Streptavidin | AlexaFluor 488 | N/A | Invitrogen | S11223 |
| Streptavidin | AlexaFluor 546 | N/A | Invitrogen | S11225 |
| Poly (I:C) | N/A | N/A | InvivoGen | tlrl-pic |
| Guava autophagy LC3 assay kit | FITC | 4E12 | Luminex | FCCH100171 |
| CD11b-coupled Microbeads | N/A | N/A | Miltenyi Biotec | 130-049-601 |
| 2-NBDG | N/A | N/A | Sigma-Aldrich | 72987 |
| Aluminium hydroxide | N/A | N/A | Sigma-Aldrich | 239186 |
| Etomoxir | N/A | N/A | Sigma-Aldrich | E1905 |
| Ferrostatin-1 | N/A | N/A | Sigma-Aldrich | SML0583 |
| Wortmannin | N/A | N/A | Sigma-Aldrich | W1628 |
| Fast SYBR green master mix | N/A | N/A | Thermo Fisher Scientific | 4385612 |
| RevertAid H minus first strand cDNA synthesis kit | N/A | N/A | Thermo Fisher Scientific | K1632 |
| GLUT1 | Uncoupled | EPR3915 | Abcam | ab115730 |
| BioTracker FerroOrange | N/A | N/A | Sigma-Aldrich | SCT210 |
| BD Phosflow fix buffer I | N/A | N/A | BD Biosciences | 557870 |
| BD Phosflow Perm buffer III | N/A | N/A | BD Biosciences | 558050 |
| Phospho-4E-BP1 (pT36/pT45) | AlexaFluor 488 | M31-16 | BD Biosciences | 560287 |
| Phospho-S6 (pS244) | PE | N5-676 | BD Biosciences | 560462 |
| Phospho-Akt (pS473) | Brilliant Violet 421 | M89-61 | BD Biosciences | 562599 |