Table 1.

Reagents

Reagent or resourceSourceIdentifier
Antibodies 
Mouse monoclonal anti-Notch, extracellular domain (ECD) (Drosophila) Developmental Studies Hybridoma Bank (DSHB) C458.2H 
Mouse monoclonal anti-delta (Drosophila) DSHB C594.9B 
Mouse monoclonal anti-tyrosine-protein phosphatase 69D (Ptp69D) (Drosophila) DSHB 3F11 
Rabbit polyclonal anti-PHB Sigma-Aldrich HPA003280 
Rabbit polyclonal anti-EXOC1 Sigma-Aldrich HPA037706 
Rabbit polyclonal anti-EXOC3 Sigma-Aldrich HPA037880 
Rabbit polyclonal anti-EXOC6 Sigma-Aldrich HPA036285 
Mouse monoclonal anti-ATP5A [15H4C4] Abcam Ab14748 
Rabbit polyclonal anti-phospho-Histone H3 (Ser10) Cell Signaling Technology 9701S 
Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 Thermo Fisher Scientific A32731 
Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 Thermo Fisher Scientific A32723 
Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 Thermo Fisher Scientific A32740 
Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 Thermo Fisher Scientific A32742 
Mouse monoclonal anti-PIP2 Thermo Fisher Scientific 2C11 
Bacterial and virus strains 
Biological samples   
Chemicals, peptides, and recombinant proteins 
Dihydroethidium (DHE) Sigma-Aldrich 37291; CAS: 104821-25-2 
Tetramethylrhodamine ethyl ester perchlorate (TMRE) Sigma-Aldrich 87917; CAS: 115532-52-0 
Critical commercial assays 
ATP determination kit Thermo Fisher Scientific A22066 
Mem-PER plus membrane protein extraction kit Thermo Fisher Scientific 89842 
D-Alanine Sigma-Aldrich A7377 
Deposited data 
Massive.ucsd.edu  MSV000091074 
NCBI-GEO  GSE223669 
NCBI-GEO  GSE227554 
Experimental models: Cell lines 
Human: MCF7 ATCC HTB-22 
Mouse: 3T3 ATCC CRL-1658 
HEk293 ATCC CRL-1573 
BT549 ATCC HTB -122 
Experimental models: Organisms/strains 
D. melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00702} attP2 Bloomington Drosophila Stock Center BDSC:32912 
D. melanogaster: RNAi of Bor: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC06013} attP40 Bloomington Drosophila Stock Center BDSC:65057 
D. melanogaster: RNAi of Fib: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ02126} attP40 Bloomington Drosophila Stock Center BDSC:42553 
D. melanogaster: RNAi of Nop5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.GL00413} attP2 Bloomington Drosophila Stock Center BDSC:35484 
D. melanogaster: Overexpression of Sod2: P{w[+mC] = UAS-Sod2.Z}1B, y [1] w [*]; Sod2[Delta02]/CyO Bloomington Drosophila Stock Center BDSC:27645 
D. melanogaster: Overexpression of Rab11: w [*]; P{w[+mC] = UAS-Rab11-GFP}2 Bloomington Drosophila Stock Center BDSC: 8506 
D. melanogaster: RNAi of Phb2: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02001} attP40/CyO Bloomington Drosophila Stock Center BDSC: 40835 
D. melanogaster: RNAi of CCT1: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00639} attP2 Bloomington Drosophila Stock Center BDSC: 32854 
D. melanogaster: RNAi of Proteasome β5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00119} attP2 Bloomington Drosophila Stock Center BDSC: 34810 
D. melanogaster: RNAi of Proteasome α7: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00068} attP2 Bloomington Drosophila Stock Center BDSC: 33660 
D. melanogaster: RNAi of Hsc70-4: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00152} attP2/TM3, Sb [1] Bloomington Drosophila Stock Center BDSC: 34836 
D. melanogaster: RNAi of ND75: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00853} attP2 Bloomington Drosophila Stock Center BDSC: 33910 
D. melanogaster: RNAi of Cype: y (1), v (1); UAS-cype-RNAi Lab stock  
D. melanogaster: RNAi of Tom22: y (1), v (1); UAS-tom22-RNAi (HMC04737) Lab stock  
D. melanogaster: RNAi of ATPSynCf6: y (1), v (1); UAS-ATPSynCf6-RNAi (HMC03238) Lab stock  
Strains 
D. melanogaster RNAi of skittles/PIP5K: y (1), v (1); UAS-PIP5K-RNAi (GL00072) Bloomington Drosophila Stock Center BDSC: 33910 
D. melanogaster y(1), v(1); Rab11(S25N)-YFP Bloomington Drosophila Stock Center BDSC: 33910 
D. melanogaster. w(1,118);Tub-roGFP TM3 Bloomington Drosophila Stock Center BDSC:68669 
D.Melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00399} attP2   
Recombinant DNA 
Software and algorithms 
Proteome discoverer v2.4 SP1   
Sequest HT   
Fiji   
GraphPad Prism 10   
MetaboAnalyst 6.0   
Sciex SIMCA 
Other   
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