Reagents
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Notch, extracellular domain (ECD) (Drosophila) | Developmental Studies Hybridoma Bank (DSHB) | C458.2H |
| Mouse monoclonal anti-delta (Drosophila) | DSHB | C594.9B |
| Mouse monoclonal anti-tyrosine-protein phosphatase 69D (Ptp69D) (Drosophila) | DSHB | 3F11 |
| Rabbit polyclonal anti-PHB | Sigma-Aldrich | HPA003280 |
| Rabbit polyclonal anti-EXOC1 | Sigma-Aldrich | HPA037706 |
| Rabbit polyclonal anti-EXOC3 | Sigma-Aldrich | HPA037880 |
| Rabbit polyclonal anti-EXOC6 | Sigma-Aldrich | HPA036285 |
| Mouse monoclonal anti-ATP5A [15H4C4] | Abcam | Ab14748 |
| Rabbit polyclonal anti-phospho-Histone H3 (Ser10) | Cell Signaling Technology | 9701S |
| Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 | Thermo Fisher Scientific | A32731 |
| Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 | Thermo Fisher Scientific | A32723 |
| Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 | Thermo Fisher Scientific | A32740 |
| Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 | Thermo Fisher Scientific | A32742 |
| Mouse monoclonal anti-PIP2 | Thermo Fisher Scientific | 2C11 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Dihydroethidium (DHE) | Sigma-Aldrich | 37291; CAS: 104821-25-2 |
| Tetramethylrhodamine ethyl ester perchlorate (TMRE) | Sigma-Aldrich | 87917; CAS: 115532-52-0 |
| Critical commercial assays | ||
| ATP determination kit | Thermo Fisher Scientific | A22066 |
| Mem-PER plus membrane protein extraction kit | Thermo Fisher Scientific | 89842 |
| D-Alanine | Sigma-Aldrich | A7377 |
| Deposited data | ||
| Massive.ucsd.edu | MSV000091074 | |
| NCBI-GEO | GSE223669 | |
| NCBI-GEO | GSE227554 | |
| Experimental models: Cell lines | ||
| Human: MCF7 | ATCC | HTB-22 |
| Mouse: 3T3 | ATCC | CRL-1658 |
| HEk293 | ATCC | CRL-1573 |
| BT549 | ATCC | HTB -122 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00702} attP2 | Bloomington Drosophila Stock Center | BDSC:32912 |
| D. melanogaster: RNAi of Bor: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC06013} attP40 | Bloomington Drosophila Stock Center | BDSC:65057 |
| D. melanogaster: RNAi of Fib: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ02126} attP40 | Bloomington Drosophila Stock Center | BDSC:42553 |
| D. melanogaster: RNAi of Nop5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.GL00413} attP2 | Bloomington Drosophila Stock Center | BDSC:35484 |
| D. melanogaster: Overexpression of Sod2: P{w[+mC] = UAS-Sod2.Z}1B, y [1] w [*]; Sod2[Delta02]/CyO | Bloomington Drosophila Stock Center | BDSC:27645 |
| D. melanogaster: Overexpression of Rab11: w [*]; P{w[+mC] = UAS-Rab11-GFP}2 | Bloomington Drosophila Stock Center | BDSC: 8506 |
| D. melanogaster: RNAi of Phb2: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02001} attP40/CyO | Bloomington Drosophila Stock Center | BDSC: 40835 |
| D. melanogaster: RNAi of CCT1: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00639} attP2 | Bloomington Drosophila Stock Center | BDSC: 32854 |
| D. melanogaster: RNAi of Proteasome β5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00119} attP2 | Bloomington Drosophila Stock Center | BDSC: 34810 |
| D. melanogaster: RNAi of Proteasome α7: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00068} attP2 | Bloomington Drosophila Stock Center | BDSC: 33660 |
| D. melanogaster: RNAi of Hsc70-4: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00152} attP2/TM3, Sb [1] | Bloomington Drosophila Stock Center | BDSC: 34836 |
| D. melanogaster: RNAi of ND75: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00853} attP2 | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster: RNAi of Cype: y (1), v (1); UAS-cype-RNAi | Lab stock | |
| D. melanogaster: RNAi of Tom22: y (1), v (1); UAS-tom22-RNAi (HMC04737) | Lab stock | |
| D. melanogaster: RNAi of ATPSynCf6: y (1), v (1); UAS-ATPSynCf6-RNAi (HMC03238) | Lab stock | |
| Strains | ||
| D. melanogaster RNAi of skittles/PIP5K: y (1), v (1); UAS-PIP5K-RNAi (GL00072) | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster y(1), v(1); Rab11(S25N)-YFP | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster. w(1,118);Tub-roGFP TM3 | Bloomington Drosophila Stock Center | BDSC:68669 |
| D.Melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00399} attP2 | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| Proteome discoverer v2.4 SP1 | ||
| Sequest HT | ||
| Fiji | ||
| GraphPad Prism 10 | ||
| MetaboAnalyst 6.0 | ||
| Sciex SIMCA | ||
| Other | ||
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Notch, extracellular domain (ECD) (Drosophila) | Developmental Studies Hybridoma Bank (DSHB) | C458.2H |
| Mouse monoclonal anti-delta (Drosophila) | DSHB | C594.9B |
| Mouse monoclonal anti-tyrosine-protein phosphatase 69D (Ptp69D) (Drosophila) | DSHB | 3F11 |
| Rabbit polyclonal anti-PHB | Sigma-Aldrich | HPA003280 |
| Rabbit polyclonal anti-EXOC1 | Sigma-Aldrich | HPA037706 |
| Rabbit polyclonal anti-EXOC3 | Sigma-Aldrich | HPA037880 |
| Rabbit polyclonal anti-EXOC6 | Sigma-Aldrich | HPA036285 |
| Mouse monoclonal anti-ATP5A [15H4C4] | Abcam | Ab14748 |
| Rabbit polyclonal anti-phospho-Histone H3 (Ser10) | Cell Signaling Technology | 9701S |
| Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 | Thermo Fisher Scientific | A32731 |
| Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 488 | Thermo Fisher Scientific | A32723 |
| Goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 | Thermo Fisher Scientific | A32740 |
| Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor plus 594 | Thermo Fisher Scientific | A32742 |
| Mouse monoclonal anti-PIP2 | Thermo Fisher Scientific | 2C11 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Dihydroethidium (DHE) | Sigma-Aldrich | 37291; CAS: 104821-25-2 |
| Tetramethylrhodamine ethyl ester perchlorate (TMRE) | Sigma-Aldrich | 87917; CAS: 115532-52-0 |
| Critical commercial assays | ||
| ATP determination kit | Thermo Fisher Scientific | A22066 |
| Mem-PER plus membrane protein extraction kit | Thermo Fisher Scientific | 89842 |
| D-Alanine | Sigma-Aldrich | A7377 |
| Deposited data | ||
| Massive.ucsd.edu | MSV000091074 | |
| NCBI-GEO | GSE223669 | |
| NCBI-GEO | GSE227554 | |
| Experimental models: Cell lines | ||
| Human: MCF7 | ATCC | HTB-22 |
| Mouse: 3T3 | ATCC | CRL-1658 |
| HEk293 | ATCC | CRL-1573 |
| BT549 | ATCC | HTB -122 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00702} attP2 | Bloomington Drosophila Stock Center | BDSC:32912 |
| D. melanogaster: RNAi of Bor: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMC06013} attP40 | Bloomington Drosophila Stock Center | BDSC:65057 |
| D. melanogaster: RNAi of Fib: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ02126} attP40 | Bloomington Drosophila Stock Center | BDSC:42553 |
| D. melanogaster: RNAi of Nop5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.GL00413} attP2 | Bloomington Drosophila Stock Center | BDSC:35484 |
| D. melanogaster: Overexpression of Sod2: P{w[+mC] = UAS-Sod2.Z}1B, y [1] w [*]; Sod2[Delta02]/CyO | Bloomington Drosophila Stock Center | BDSC:27645 |
| D. melanogaster: Overexpression of Rab11: w [*]; P{w[+mC] = UAS-Rab11-GFP}2 | Bloomington Drosophila Stock Center | BDSC: 8506 |
| D. melanogaster: RNAi of Phb2: y [1] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS02001} attP40/CyO | Bloomington Drosophila Stock Center | BDSC: 40835 |
| D. melanogaster: RNAi of CCT1: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00639} attP2 | Bloomington Drosophila Stock Center | BDSC: 32854 |
| D. melanogaster: RNAi of Proteasome β5: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00119} attP2 | Bloomington Drosophila Stock Center | BDSC: 34810 |
| D. melanogaster: RNAi of Proteasome α7: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00068} attP2 | Bloomington Drosophila Stock Center | BDSC: 33660 |
| D. melanogaster: RNAi of Hsc70-4: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00152} attP2/TM3, Sb [1] | Bloomington Drosophila Stock Center | BDSC: 34836 |
| D. melanogaster: RNAi of ND75: y [1] sc[*] v [1] sev[21]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00853} attP2 | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster: RNAi of Cype: y (1), v (1); UAS-cype-RNAi | Lab stock | |
| D. melanogaster: RNAi of Tom22: y (1), v (1); UAS-tom22-RNAi (HMC04737) | Lab stock | |
| D. melanogaster: RNAi of ATPSynCf6: y (1), v (1); UAS-ATPSynCf6-RNAi (HMC03238) | Lab stock | |
| Strains | ||
| D. melanogaster RNAi of skittles/PIP5K: y (1), v (1); UAS-PIP5K-RNAi (GL00072) | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster y(1), v(1); Rab11(S25N)-YFP | Bloomington Drosophila Stock Center | BDSC: 33910 |
| D. melanogaster. w(1,118);Tub-roGFP TM3 | Bloomington Drosophila Stock Center | BDSC:68669 |
| D.Melanogaster: RNAi of Phb1: y [1] sc[*] v [1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00399} attP2 | ||
| Recombinant DNA | ||
| Software and algorithms | ||
| Proteome discoverer v2.4 SP1 | ||
| Sequest HT | ||
| Fiji | ||
| GraphPad Prism 10 | ||
| MetaboAnalyst 6.0 | ||
| Sciex SIMCA | ||
| Other | ||