Table 4.

Chemicals, peptides, and recombinant proteins

SourceIdentifier
X-tremeGENE 9 DNA Transfection Reagent Merck Cat# 6365809001 
ANTI-FLAG M2 Affinity gel Merck Cat# A2220-5ML 
Polybrene Santa Cruz Biotechnology Cat# sc-134220 
LysoTracker Deep Red Invitrogen Cat# L12492 
Human Transferrin AlexaFluor647 Jackson ImmunoResearch Cat# 009-600-050 
Glutathione Sepharose 4B Merck Cat# GE17-0756-01 
GFP-Trap agarose Proteintech/Chromotek Cat# gta 
SUMOstar protease LifeSensors Cat# 4110-500UNITS 
Amylose Resin New England Biolabs Cat# E8021L 
Pierce Cell surface Biotinylation and Isolation kit Thermo Fisher Scientific Cat# A44390 
DirectPCR lysis reagent Nordic BioSite Cat# 250-301-C 
cOmplete, EDTA-free Protease inhibitor Cocktail Roche Cat# 5056489001 
Supersignal west dura extended duration substrate Thermo Fisher Scientific Cat# 34075 
SourceIdentifier
X-tremeGENE 9 DNA Transfection Reagent Merck Cat# 6365809001 
ANTI-FLAG M2 Affinity gel Merck Cat# A2220-5ML 
Polybrene Santa Cruz Biotechnology Cat# sc-134220 
LysoTracker Deep Red Invitrogen Cat# L12492 
Human Transferrin AlexaFluor647 Jackson ImmunoResearch Cat# 009-600-050 
Glutathione Sepharose 4B Merck Cat# GE17-0756-01 
GFP-Trap agarose Proteintech/Chromotek Cat# gta 
SUMOstar protease LifeSensors Cat# 4110-500UNITS 
Amylose Resin New England Biolabs Cat# E8021L 
Pierce Cell surface Biotinylation and Isolation kit Thermo Fisher Scientific Cat# A44390 
DirectPCR lysis reagent Nordic BioSite Cat# 250-301-C 
cOmplete, EDTA-free Protease inhibitor Cocktail Roche Cat# 5056489001 
Supersignal west dura extended duration substrate Thermo Fisher Scientific Cat# 34075 

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