Antibodies, DNA imager oligomers, and drug treatments
| Antibody/oligo . | Identifier . | Experiments used . | Dilutions . |
|---|---|---|---|
| Sheep Anti-LAMP1 | RRID:AB_1026176 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Mouse Anti-LAMP2 | RRID:AB_626858 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Mouse Anti-CD63 | RRID:AB_2884028 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Goat anti-Cathepsin D | RRID:AB_2087218 | DNA-PAINT; WB | 1:100; 1:1,000 |
| Rabbit Anti-TMEM192 | RRID:AB_3095683 | DNA-PAINT; widefield imaging; WB | 1:25; 1:500; 1:1,000 |
| Rabbit Anti-NPC1 | RRID:AB_10001101 | DNA-PAINT | 1:100 |
| Rabbit Anti-LAMTOR4 | RRID:AB_2798129 | DNA-PAINT; widefield imaging; WB | 1:100; 1:500 |
| Mouse Anti-TOM20 | RRID:AB_628381 | DNA-PAINT | 1:100 |
| Mouse Anti-P230 (TGN) | RRID:AB_398808 | DNA-PAINT | 1:25 |
| Mouse Anti-flag | RRID:AB_262044 | Widefield imaging | 1:500 |
| Rabbit Anti-HA | RRID:AB_307019 | Widefield imaging | 1:500 |
| Rabbit Anti-EEA1 | RRID:AB_2096811 | DNA-PAINT | 1:100 |
| Rabbit Anti-TMEM192 (alternative antibody) | RRID:AB_2902909 | DNA-PAINT | 1:100 |
| Rabbit Anti-NPC1 | RRID:AB_2734695 | WB | 1:1,000 |
| Mouse Anti-GAPDH | RRID:AB_2107436 | WB | 1:1,000 |
| Rabbit Anti-TOM20 | RRID:AB_2207530 | DNA-PAINT | 1:100 |
| Rabbit anti-tubulin, acetylated | RRID:AB_477585 | DNA-PAINT | 1:100 |
| Anti-Mouse IgG+ Docking site 1 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 1:100 |
| Anti-Rabbit IgG+ Docking site 2 | Massive Photonics Massive-AB 2-Plex | DNA-PAINT | 1:100 |
| Donkey anti-sheep IgG+ Docking site E2 | RRID:AB_2340704 | DNA-PAINT | 1:100 |
| Donkey anti-Goat IgG+ Docking site E2 | RRID:AB_2340384 | DNA-PAINT | 1:100 |
| Tag-Q Anti-GFP single-Domain Antibody+ Docking site 3 | Massive Photonics, Massive-TagQ Anti-GFP | DNA-PAINT | 1:100 |
| Anti-Rabbit single-Domain Antibody+ Docking site F1 | Massive Photonics, custom order | DNA-PAINT | 1:25 |
| Anti-Rabbit single-Domain Antibody+ Docking site F2 | Massive Photonics, Massive SDAB fast one-Plex | DNA-PAINT | 1:25 |
| Donkey anti-Mouse IgG+ Alexa fluor 647 | RRID:AB_162542 | Widefield imaging | 1:500 |
| Goat anti-Rabbit IgG+ Cy3 | RRID:AB_10563288 | Widefield imaging | 1:500 |
| IRDye800CW Donkey anti-Rabbit | RRID:AB_621848 | WB | 1:10,000 |
| IRDye680RD Donkey anti-Mouse | RRID:AB_10953628 | WB | 1:10,000 |
| IRDye800CW Donkey anti-Goat | RRID:AB_2687553 | WB | 1:10,000 |
| Imager oligo for Docking site 1+ Cy3B or ATTO655 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 500 pM |
| Imager oligo for Docking site 2+ Cy3B or ATTO655 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 500 pM |
| Imager oligo for Docking site 3+ Cy3B or ATTO655 | Massive Photonics, Massive-TagQ Anti-GFP | DNA-PAINT | 100–300 pM |
| Imager oligo for Docking site E2+ Cy3 | IDT, custom order | DNA-PAINT | 500 pM |
| Imager oligo for Docking site F1+ Cy3B or ATTO655 | Massive Photonics, custom order | DNA-PAINT | 1.5 nM |
| Imager oligo for Docking site F2+ Cy3B or ATTO655 | Massive Photonics, Massive SDAB fast one-Plex | DNA-PAINT | 1.5 nM |
| EN6 | Tocris Biosciences #7474 | DNA-PAINT; WB | 15 µM, 16 h |
| BafA1 | Sigma-Aldrich #SML1661 | DNA-PAINT; WB | 100 nM, 16 h |
| Antibody/oligo . | Identifier . | Experiments used . | Dilutions . |
|---|---|---|---|
| Sheep Anti-LAMP1 | RRID:AB_1026176 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Mouse Anti-LAMP2 | RRID:AB_626858 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Mouse Anti-CD63 | RRID:AB_2884028 | DNA-PAINT; widefield imaging | 1:100; 1:500 |
| Goat anti-Cathepsin D | RRID:AB_2087218 | DNA-PAINT; WB | 1:100; 1:1,000 |
| Rabbit Anti-TMEM192 | RRID:AB_3095683 | DNA-PAINT; widefield imaging; WB | 1:25; 1:500; 1:1,000 |
| Rabbit Anti-NPC1 | RRID:AB_10001101 | DNA-PAINT | 1:100 |
| Rabbit Anti-LAMTOR4 | RRID:AB_2798129 | DNA-PAINT; widefield imaging; WB | 1:100; 1:500 |
| Mouse Anti-TOM20 | RRID:AB_628381 | DNA-PAINT | 1:100 |
| Mouse Anti-P230 (TGN) | RRID:AB_398808 | DNA-PAINT | 1:25 |
| Mouse Anti-flag | RRID:AB_262044 | Widefield imaging | 1:500 |
| Rabbit Anti-HA | RRID:AB_307019 | Widefield imaging | 1:500 |
| Rabbit Anti-EEA1 | RRID:AB_2096811 | DNA-PAINT | 1:100 |
| Rabbit Anti-TMEM192 (alternative antibody) | RRID:AB_2902909 | DNA-PAINT | 1:100 |
| Rabbit Anti-NPC1 | RRID:AB_2734695 | WB | 1:1,000 |
| Mouse Anti-GAPDH | RRID:AB_2107436 | WB | 1:1,000 |
| Rabbit Anti-TOM20 | RRID:AB_2207530 | DNA-PAINT | 1:100 |
| Rabbit anti-tubulin, acetylated | RRID:AB_477585 | DNA-PAINT | 1:100 |
| Anti-Mouse IgG+ Docking site 1 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 1:100 |
| Anti-Rabbit IgG+ Docking site 2 | Massive Photonics Massive-AB 2-Plex | DNA-PAINT | 1:100 |
| Donkey anti-sheep IgG+ Docking site E2 | RRID:AB_2340704 | DNA-PAINT | 1:100 |
| Donkey anti-Goat IgG+ Docking site E2 | RRID:AB_2340384 | DNA-PAINT | 1:100 |
| Tag-Q Anti-GFP single-Domain Antibody+ Docking site 3 | Massive Photonics, Massive-TagQ Anti-GFP | DNA-PAINT | 1:100 |
| Anti-Rabbit single-Domain Antibody+ Docking site F1 | Massive Photonics, custom order | DNA-PAINT | 1:25 |
| Anti-Rabbit single-Domain Antibody+ Docking site F2 | Massive Photonics, Massive SDAB fast one-Plex | DNA-PAINT | 1:25 |
| Donkey anti-Mouse IgG+ Alexa fluor 647 | RRID:AB_162542 | Widefield imaging | 1:500 |
| Goat anti-Rabbit IgG+ Cy3 | RRID:AB_10563288 | Widefield imaging | 1:500 |
| IRDye800CW Donkey anti-Rabbit | RRID:AB_621848 | WB | 1:10,000 |
| IRDye680RD Donkey anti-Mouse | RRID:AB_10953628 | WB | 1:10,000 |
| IRDye800CW Donkey anti-Goat | RRID:AB_2687553 | WB | 1:10,000 |
| Imager oligo for Docking site 1+ Cy3B or ATTO655 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 500 pM |
| Imager oligo for Docking site 2+ Cy3B or ATTO655 | Massive Photonics, Massive-AB 2-Plex | DNA-PAINT | 500 pM |
| Imager oligo for Docking site 3+ Cy3B or ATTO655 | Massive Photonics, Massive-TagQ Anti-GFP | DNA-PAINT | 100–300 pM |
| Imager oligo for Docking site E2+ Cy3 | IDT, custom order | DNA-PAINT | 500 pM |
| Imager oligo for Docking site F1+ Cy3B or ATTO655 | Massive Photonics, custom order | DNA-PAINT | 1.5 nM |
| Imager oligo for Docking site F2+ Cy3B or ATTO655 | Massive Photonics, Massive SDAB fast one-Plex | DNA-PAINT | 1.5 nM |
| EN6 | Tocris Biosciences #7474 | DNA-PAINT; WB | 15 µM, 16 h |
| BafA1 | Sigma-Aldrich #SML1661 | DNA-PAINT; WB | 100 nM, 16 h |