Plasmids and siRNAs
| Plasmid name . | Identifier . | Insert . | Species . |
|---|---|---|---|
| LAMP1-mGFP | RRID:Addgene_34831 | LAMP1 | Homo sapiens (human) |
| pRK5-LAMP1-FLAG | RRID:Addgene_71868 | LAMP1 | H. sapiens (human) |
| LAMP2-BFP | Gift from Holzbaur lab (University of Pennsylvania, Philadelphia, PA, USA) (Gallagher and Holzbaur, 2023) | LAMP2 | H. sapiens (human) |
| pMEH371 CD63 HA | RRID:Addgene_168220 | CD63 | H. sapiens (human) |
| pLJC5-Tmem192-3xHA | RRID:Addgene_102930 | TMEM192 | H. sapiens (human) |
| pcDNA 3.1(+) – hNPC1(WT)-FLAG | Custom generated by Genscript, re-cloned from RRID:Addgene_164972 | NPC1 | H. sapiens (human) |
| pRK5-FLAG-C7orf59 | RRID:Addgene_42332 | LAMTOR4 | H. sapiens (human) |
| TMEM192 siRNA | Santa Cruz Biotech #sc-89327 | siRNA to TMEM192 | H. sapiens (human) |
| Cathepsin D siRNA | Santa Cruz Biotech #sc-29239 | siRNA to Cathepsin D | H. sapiens (human) |
| Control siRNA | Santa Cruz Biotech #sc-37007 | Control siRNA | H. sapiens (human) |
| Plasmid name . | Identifier . | Insert . | Species . |
|---|---|---|---|
| LAMP1-mGFP | RRID:Addgene_34831 | LAMP1 | Homo sapiens (human) |
| pRK5-LAMP1-FLAG | RRID:Addgene_71868 | LAMP1 | H. sapiens (human) |
| LAMP2-BFP | Gift from Holzbaur lab (University of Pennsylvania, Philadelphia, PA, USA) (Gallagher and Holzbaur, 2023) | LAMP2 | H. sapiens (human) |
| pMEH371 CD63 HA | RRID:Addgene_168220 | CD63 | H. sapiens (human) |
| pLJC5-Tmem192-3xHA | RRID:Addgene_102930 | TMEM192 | H. sapiens (human) |
| pcDNA 3.1(+) – hNPC1(WT)-FLAG | Custom generated by Genscript, re-cloned from RRID:Addgene_164972 | NPC1 | H. sapiens (human) |
| pRK5-FLAG-C7orf59 | RRID:Addgene_42332 | LAMTOR4 | H. sapiens (human) |
| TMEM192 siRNA | Santa Cruz Biotech #sc-89327 | siRNA to TMEM192 | H. sapiens (human) |
| Cathepsin D siRNA | Santa Cruz Biotech #sc-29239 | siRNA to Cathepsin D | H. sapiens (human) |
| Control siRNA | Santa Cruz Biotech #sc-37007 | Control siRNA | H. sapiens (human) |