Table 1. Kinetic parameters of toxin... | Rockefeller University Press

Table 1.

Kinetic parameters of toxin association and dissociation from SPR fits

Toxin	K_ON (M⁻1 s⁻1)	K_OFF (s⁻1)	K_D (M)
PCTX1	3.81e⁷ ± 3.5e⁵ (1.03e⁷± 5.1e⁴)^{^a}	2.73e⁻⁴ ± 1.10e⁻⁶ (0.146 ± 3.60e⁻⁴)^{^a}	7.12e⁻¹² (1.42e⁻⁸)^{^a}
HI1A	6.88e⁷ ± 8.5e⁵ (7.49e⁵± 5.2e³)^{^a}	0.014 ± 1.70e⁻⁴ (0.157 ± 6.00e⁻⁴)^{^a}	1.98e⁻¹⁰ (2.09e⁻⁷)^{^a}
N	4.68e⁶ ± 1.3e⁴ (2.06e⁶± 2.7e³)^{^a}	0.007 ± 1.50e⁻⁵ (0.320 ± 1.50e⁻⁴)^{^a}	1.42e⁻⁹ (1.5e⁻⁷)^{^a}
C	2.10e⁷ ± 1.8e⁵	0.184 ± 0.0017	8.75e⁻⁹
HI1A-CN	3.61e⁷ ± 5.2e⁵	0.030 ± 4.4e⁻⁴	8.19e⁻¹⁰
S27R	4.79e⁷ ± 2.9e⁵	0.001 ± 8.10e⁻⁶	2.77e⁻¹¹
G28R	2.29e⁷ ± 4.0e⁵	0.048 ± 9.10e⁻⁴	2.11e⁻⁹
N29S	1.53e⁷ ± 2.5e⁵	0.182 ± 0.003	1.27e⁻⁸

Toxin	K_ON (M⁻1 s⁻1)	K_OFF (s⁻1)	K_D (M)
PCTX1	3.81e⁷ ± 3.5e⁵ (1.03e⁷± 5.1e⁴)^{^a}	2.73e⁻⁴ ± 1.10e⁻⁶ (0.146 ± 3.60e⁻⁴)^{^a}	7.12e⁻¹² (1.42e⁻⁸)^{^a}
HI1A	6.88e⁷ ± 8.5e⁵ (7.49e⁵± 5.2e³)^{^a}	0.014 ± 1.70e⁻⁴ (0.157 ± 6.00e⁻⁴)^{^a}	1.98e⁻¹⁰ (2.09e⁻⁷)^{^a}
N	4.68e⁶ ± 1.3e⁴ (2.06e⁶± 2.7e³)^{^a}	0.007 ± 1.50e⁻⁵ (0.320 ± 1.50e⁻⁴)^{^a}	1.42e⁻⁹ (1.5e⁻⁷)^{^a}
C	2.10e⁷ ± 1.8e⁵	0.184 ± 0.0017	8.75e⁻⁹
HI1A-CN	3.61e⁷ ± 5.2e⁵	0.030 ± 4.4e⁻⁴	8.19e⁻¹⁰
S27R	4.79e⁷ ± 2.9e⁵	0.001 ± 8.10e⁻⁶	2.77e⁻¹¹
G28R	2.29e⁷ ± 4.0e⁵	0.048 ± 9.10e⁻⁴	2.11e⁻⁹
N29S	1.53e⁷ ± 2.5e⁵	0.182 ± 0.003	1.27e⁻⁸

Smaller italicized values are using F352A hASIC1a mutation.