List of reagents
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Q5 HF DNA polymerase | NEB | Cat# M0491L |
| Gibson assembly master mix | NEB | Cat# E2611L |
| DMEM + GlutaMAX-l | GIBCO | Cat# 61965-026 |
| Dulbecco’s Phosphate-Buffered Saline (DPBS) | Corning | Cat# 21-031-CV |
| Pen strep | GIBCO | Cat# 15140-122 |
| Trypsin-EDTA | Gibco | Cat# 25200-056 |
| Fetal bovine serum | Sigma-Aldrich | Cat# F7525-500ML |
| Fibronectin | Sigma-Aldrich | Cat# F1141-2MG |
| Blasticidin | Thermo Fisher Scientific | Cat# R21001 |
| Hygromycin B | Corning | Cat# 30-240CR |
| Paclitaxel/Taxol | Thermo Fisher Scientific | Cat# P3456 |
| Paclitaxel/Taxol (for in vitro microtubule reconstitutions) | Enzo Life Sciences | Cat# BML-T104 |
| Doxycycline | Sigma-Aldrich | Cat# 24390-14-5 |
| fBSA-Au5 | Cell Microscopy Core, Utrecht University Medical Center (UMC) | https://cellmicroscopy.nl/products |
| ProtA-Au5 | Cell Microscopy Core, Utrecht UMC | https://cellmicroscopy.nl/products |
| Dynabeads MyOne carboxylic acid | Thermo Fisher Scientific | Cat# 65011 |
| Cellview cell culture dish | Greiner bio-one | Cat# 627860 |
| Quantifoil 200 mesh holey carbon R2/2 gold grids | Quantifoil Micro Tools | Not applicable |
| Quantifoil 200 mesh holey carbon R2/2 copper grids | Quantifoil Micro Tools | Not applicable |
| Whatman filter paper | Whatman | Cat# 10311610 |
| Porcine brain tubulin protein (>99% pure) | Cytoskeleton | Cat# T240 |
| Fluorescent HiLyte 647 porcine brain tubulin protein | Cytoskeleton | Cat# TL670M |
| Biotin porcine brain tubulin protein | Cytoskeleton | Cat# 333P |
| X-rhodamine porcine brain tubulin protein | Cytoskeleten | Cat# TL620M |
| PIPES | Sigma-Aldrich | Cat# P6757 |
| MgCl2 | Sigma-Aldrich | Cat# M2670 |
| EGTA | Sigma-Aldrich | Cat# E4378 |
| GpCpp (GMPCPP) | Jena Biocsciences | Cat# NU-405L |
| GTP | Sigma-Aldrich | Cat# G8877 |
| DTT | Thermo Fisher Scientific | Cat# R0861 |
| Reagent or resource . | Source . | Identifier . |
|---|---|---|
| Q5 HF DNA polymerase | NEB | Cat# M0491L |
| Gibson assembly master mix | NEB | Cat# E2611L |
| DMEM + GlutaMAX-l | GIBCO | Cat# 61965-026 |
| Dulbecco’s Phosphate-Buffered Saline (DPBS) | Corning | Cat# 21-031-CV |
| Pen strep | GIBCO | Cat# 15140-122 |
| Trypsin-EDTA | Gibco | Cat# 25200-056 |
| Fetal bovine serum | Sigma-Aldrich | Cat# F7525-500ML |
| Fibronectin | Sigma-Aldrich | Cat# F1141-2MG |
| Blasticidin | Thermo Fisher Scientific | Cat# R21001 |
| Hygromycin B | Corning | Cat# 30-240CR |
| Paclitaxel/Taxol | Thermo Fisher Scientific | Cat# P3456 |
| Paclitaxel/Taxol (for in vitro microtubule reconstitutions) | Enzo Life Sciences | Cat# BML-T104 |
| Doxycycline | Sigma-Aldrich | Cat# 24390-14-5 |
| fBSA-Au5 | Cell Microscopy Core, Utrecht University Medical Center (UMC) | https://cellmicroscopy.nl/products |
| ProtA-Au5 | Cell Microscopy Core, Utrecht UMC | https://cellmicroscopy.nl/products |
| Dynabeads MyOne carboxylic acid | Thermo Fisher Scientific | Cat# 65011 |
| Cellview cell culture dish | Greiner bio-one | Cat# 627860 |
| Quantifoil 200 mesh holey carbon R2/2 gold grids | Quantifoil Micro Tools | Not applicable |
| Quantifoil 200 mesh holey carbon R2/2 copper grids | Quantifoil Micro Tools | Not applicable |
| Whatman filter paper | Whatman | Cat# 10311610 |
| Porcine brain tubulin protein (>99% pure) | Cytoskeleton | Cat# T240 |
| Fluorescent HiLyte 647 porcine brain tubulin protein | Cytoskeleton | Cat# TL670M |
| Biotin porcine brain tubulin protein | Cytoskeleton | Cat# 333P |
| X-rhodamine porcine brain tubulin protein | Cytoskeleten | Cat# TL620M |
| PIPES | Sigma-Aldrich | Cat# P6757 |
| MgCl2 | Sigma-Aldrich | Cat# M2670 |
| EGTA | Sigma-Aldrich | Cat# E4378 |
| GpCpp (GMPCPP) | Jena Biocsciences | Cat# NU-405L |
| GTP | Sigma-Aldrich | Cat# G8877 |
| DTT | Thermo Fisher Scientific | Cat# R0861 |