Table 2.

Primers used for plasmid construction

PrimersSequences (5′-3′)
Vector-VPS13B-F 5′-TCG​AGC​TCA​AGC​TTC​GAA​TTC​GCC​ACC​ATG​CTG​GAG​TCA​TAT​G-3′ 
VPS13B(1265AA)-R 5′-CCT​TTG​CTC​ATA​ACT​GGA​GAA​GTA​GGA​ACA​GGC​C-3′ 
VPS13B(1265AA)^sfGFP-F 5′-TCT​CCA​GTT​ATG​AGC​AAA​GGA​GAA​GAA​CTT​TTC​A-3′ 
SfGFP^VPS13B(1266AA)-R 5′-TGC​CTA​TAC​TGC​TTC​TTT​TGT​AGA​GCT​CAT​CCA​TG-3′ 
VPS13B(1266AA)-F 5′-AGA​AGC​AGT​ATA​GGC​ACA​GCT​CC-3′ 
Vector-VPS13B-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​AGG​AAA​CCC​TTT​CCT​CAG​G-3′ 
GFP-VPS13B-NT(1–1,500)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​CTG​GAG​TCA​TAT​GTA​ACT​CCA​A-3′ 
GFP-VPS13B-NT(1–1,500)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​ATC​AAA​AGC​TTG​TGC​TGA​AA-3′ 
GFP-VPS13B-N,S560D-F 5′-GAC​TTT​TCT​GAT​GGG​AAA​AGT​GAA​GAT​TTG​GGA​A-3′ 
GFP-VPS13B-N,S560D-R 5′-TTC​CCA​TCA​GAA​AAG​TCT​TGT​TGA​TTT​GTG​GAA​C-3′ 
GFP-VPS13B-N,S503D,P504A-F 5′-GAT​TAC​CGA​GAT​GCC​GAA​AAT​AAT​GGT​ACT​CGC​GCA​GA-3′ 
GFP-VPS13B-N,S503D,P504A-R 5′-TTC​GGC​ATC​TCG​GTA​ATC​AAA​CAA​TGA​ATT​TGT​AA-3′ 
GFP-VPS13B-N,S1403D-F 5′-CTT​CCT​TGA​CTG​TAC​TGA​CAA​GCT​GAA​CAG​ACG​C-3′ 
GFP-VPS13B-N,S1403D-R 5′-CAG​TAC​AGT​CAA​GGA​AGA​CAC​CTC​CAC​ATT​GC-3′ 
GFP-VPS13B-N,S14033D-F 5′-GCT​TCT​TTC​CTG​ACA​CAA​CTA​CAA​AAC​TTC​TAG​ATG​GCA​CTC-3′ 
GFP-VPS13B-N,S14033D-R 5′-TGT​GTC​AGG​AAA​GAA​GCC​AGA​GCA​ATT​ACT​GA-3′ 
Halo-VAPB-F 5′-GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​AAG​GTG​GAG​CAG​G-3′ 
Halo-VAPB-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​CAA​GGC​AAT​CTT​CCC​AAT​AAT​T-3′ 
Halo-MOSPD2-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCA​GAG​AAT​CAC​GCC​C-3′ 
Halo-MOSPD2-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​ACT​GTA​CAA​TAA​ATA​GAA​GAA​AGA​GG-3′ 
MGAT2-Halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGG​TTC​CGC​ATC​TAC​AAA​CG-3′ 
MGAT2-Halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​CTG​CAG​TCT​TCT​ATA​ACT​TTT​ACA​GAG​TTC-3′ 
GFP-Sec31A-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​AAG​TTA​AAG​GAA​GTA​GAT​CGT​ACA​GC-3′ 
GFP-Sec31A-R 5′-GCC​TGA​GTA​ATT​AAA​CCA​TCA​ATG​TCC​CCA​CTG​A-3′ 
mCherry-Sec31A-F 5′-CTG​CAG​TCG​ACG​GTA​CCG​CGG​ATG​AAG​TTA​AAG​GAA​GTA​GAT​CGT​ACA​GC-3′ 
mCherry-Sec31A-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GAC​ACC​CAG​CTT​ATT​GGC​C-3′ 
GFP-Sec23IP-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
GFP-Sec23IP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
Halo-Sec23IP-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
Halo-Sec23IP-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
GST-Sec23IP-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
GST-Sec23IP-R 5′-TCA​GTC​AGT​CAC​GAT​GAA​TTC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
GST-Sec23IP NT-R 5′-CGA​TGA​ATT​CTC​ACT​CTA​GTT​TTT​CAC​TGA​ACT​CCT​CAG​TAT-3′ 
GST-Sec23IP ΔNT-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​GCT​GAA​TAT​AAA​AAA​GCT​GTA​ACC​ACT-3′ 
Halo-Sec23IP NT-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​CTC​TAG​TTT​TTC​ACT​GAA​CTC​CTC​AG-3′ 
Halo-Sec23IP ΔNT-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​GCT​GAA​TAT​AAA​AAA​GCT​GTA​ACC​ACT-3′ 
Halo-Sec23IP(Δ 368–642)-F 5′-AGA​TCT​CGA​GCT​CAA​GCT​TCG​ATG​ACA​GTC​GTA​GGG​AAC​CCT​CG-3′ 
Halo-Sec23IP Δ 368–642)-R 5′-AAA​ACT​AGA​GAA​TAA​AGA​AGT​CCT​AAC​TTT-3′ 
Halo-Sec23IP(Δ SAM)-F 5′-GTT​GAA​AAG​AAG​GCA​GCG​TCA​GAA​AAG​AAG​GCA-3′ 
Halo-Sec23IP Δ SAM)-R 5′-CGC​TGC​CTT​CTT​TTC​AAC​AAC​AAG​GTC​ATA​CGA​CTC​A-3′ 
Halo-Sec23IP(Δ 708–779AA)-F 5′-AGC​CAA​ACT​GGA​TTT​TGA​ACC​AGA​GAT​ATT-3′ 
Halo-Sec23IP(Δ 708–779AA)-R 5′-GTT​CAA​AAT​CCA​GTT​TGG​CTG​CTT​TAT​GTT-3′ 
Halo-Sec23IP Δ DDHD)-F 5′-GCT​TAC​AAC​TCA​TTA​ACA​ATG​AAC​ATT​AGT​CCA​GAA​CAG​CC-3′ 
Halo-Sec23IP(Δ DDHD)-R 5′-GTT​AAT​GAG​TTG​TAA​GCA​ACA​GAA​ACC​TGT​CC-3′ 
Halo-Sec23IP Δ 989–1000AA)-F 5′-TAT​CGA​TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​CT-3′ 
Halo-Sec23IP Δ 989–1000AA)-FR 5′-GTG​GAT​CCT​CAT​CGA​TAA​ATT​TCT​TTA​AGT​AGT​AAC​AGA​GC-3′ 
GFP-VPS13B-VAB-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​GTA​GAG​GAG​TTG​GTC​TTC​AGC​CA-3′ 
GFP-VPS13B-VAB-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​TAA​CCG​AGC​AGG​TTT​TAA​TTC​AAA-3′ 
GFP-VPS13B-PH-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​TGC​TTG​CTG​CTG​ACA​TCA​GAA-3′ 
GFP-VPS13B-PH-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​CTT​TGT​GAT​GTA​GTC​CAC​CAG​T-3′ 
VPS13B(Δ PH)-GFP-F 5′-CAT​GAA​GGG​ACA​TCT​TGT​CAC​CTG​GCC​CCC​AG-3′ 
VPS13B(Δ PH)-GFP-R 5′-CAA​GAT​GTC​CCT​TCA​TGC​TCC​TGG​CCT​GAG​CC-3′ 
VPS13B G2729R-F 5′-CAT​CTG​TAG​AAG​ACA​GAT​CAT​CTG​TAG​TTA​CTT​GTC​TCA​A-3′ 
VPS13B G2729R-R 5′-TCT​GTC​TTC​TAC​AGA​TGA​TAA​TCT​GTT​TTT​GTA​CTC​CA-3′ 
VPS13B I2820T-F 5′-CCA​CTA​TTT​ATG​TCT​GGT​GCA​CAG​TTT​TGA​CT-3′ 
VPS13B I2820T-R 5′-CCA​GAC​ATA​AAT​AGT​GGA​ACT​GTT​TGA​AGA​TGG​CAC-3′ 
VPS13B Y2822C-F 5′-ATT​TGT​GTC​TGG​TGC​ACA​GTT​TTG​ACT​TTA​GA-3′ 
VPS13B Y2822C-R 5′-GTG​CAC​CAG​ACA​CAA​ATA​ATG​GAA​CTG​TTT​GAA​GAT​GGC-3′ 
VPS13B N2993S-F 5′-GCT​TAT​CTC​CGA​ATC​CAA​ATG​GGA​CCT​CTG​GC-3′ 
VPS13B N2993S-R 5′-TGG​ATT​CGG​AGA​TAA​GCA​GGG​CCC​AGG​GCA​GA-3′ 
VPS13B A3033E-F 5′-ACT​GGG​AAA​ATA​CAA​ACA​CTG​TGC​ACA​AGT​CAG-3′ 
VPS13B A3033E-R 5′-GTT​TGT​ATT​TTC​CCA​GTA​TAT​TCC​AAT​TTG​AAA​AGC-3′ 
VPS13B N3113Y-F 5′-CTA​CAA​TAC​ACC​ATA​TCA​AAT​ATT​TTA​TAA​ACC​AC-3′ 
VPS13B N3113Y-R 5′-GAT​ATG​GTG​TAT​TGT​AGA​TTA​TTG​TAG​TCT​TGT​CTT​CAA​TCT​GAA​T-3′ 
VPS13B S3142R-F 5′-CCA​GAC​AGG​GCT​ACT​TTT​AGC​ATT​TGC​CCA​GG-3′ 
VPS13B S3142R-R 5′-AAA​GTA​GCC​CTG​TCT​GGA​ACA​CGA​AAA​TAC​TCC-3′ 
VPS13B R3223W-F 5′-CGG​GAA​TTT​CTG​GGA​AAA​TGG​ATT​CTG​TAC​CAG​GGC-3′ 
VPS13B R3223W-R 5′-TTT​CCC​AGA​AAT​TCC​CGA​GGG​GTA​CTG​CCA​CA-3′ 
VPS13B S3328R-F 5′-AGT​GGA​GGG​ATG​CCA​TTG​ACA​TCA​ACA​GTC​AG-3′ 
VPS13B S3328R-R 5′-AAT​GGC​ATC​CCT​CCA​CTC​AGT​TGT​TAC​TTC​ATC​TAG​AGG-3′ 
VPS13B G3432R-F 5′-TCC​CTA​GGG​AAG​AGC​CTG​TGG​CTG​CGT​TGT​TT-3′ 
VPS13B G3432R-R 5′-AGG​CTC​TTC​CCT​AGG​GAG​GGG​ACC​AGC​TCC​CG-3′ 
VPS13B V3470M-F 5′-TGT​CTT​AAT​GTG​CCA​GGG​AGA​AAA​AGC​AGA​AC-3′ 
VPS13B V3470M-R 5′-CCT​GGC​ACA​TTA​AGA​CAG​CAA​AGT​GGA​AAT​TGG​A-3′ 
GFP-VPS13B(VAB R1)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​TCT​TCA​GCC​ATT​TTG​TGA-3′ 
GFP-VPS13B(VAB R1)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​TCC​ACA​GAT​GAT​AAT​CTG​TTT​TTG​T-3′ 
GFP-VPS13B(VAB R2)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGC​AGA​TCA​TCT​GTA​GTT​ACT​TGT​CTC-3′ 
GFP-VPS13B(VAB R2)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GCT​GAA​CAC​AAT​CAT​TCG​TTG-3′ 
GFP-VPS13B(VAB R3)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGC​TTT​TTA​TCA​TGA​GGA​GTC​ATC-3′ 
GFP-VPS13B(VAB R3)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​CAG​AAG​TTC​TAT​CAA​CAA​AGT​TCT​AAC​A-3′ 
GFP-VPS13B(VAB R4)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​CCC​TGC​TTA​TCA​ATG​AA-3′ 
GFP-VPS13B(VAB R4)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GTC​TTC​AAT​CTG​AAT​AAT​TTG​TAT​ACC​TT-3′ 
GFP-VPS13B(VAB R5)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGA​CTA​CAA​TAA​TCA​ATA​ATA​CAC​CAT​ATC​A-3′ 
GFP-VPS13B(VAB R5)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​TTC​TGA​GAG​GGT​TAA​ATA​AGT​CAC​TC-3′ 
GFP-VPS13B(VAB R6)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​TAA​TTA​TCC​ACA​ATA​GAT​GTC​CA-3′ 
GFP-VPS13B(VAB R6)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​AGT​GAT​GAA​GAC​TGT​GCC​ACA-3′ 
GFP-VPS13B(VAB Δ R1)-F 5′-AGG​AGT​TGA​GAC​AGA​TCA​TCT​GTA​GTT​ACT​TGT​CTC​AA-3′ 
GFP-VPS13B(VAB Δ R1)-R 5′-GAT​CTG​TCT​CAA​CTC​CTC​TAC​AGA​TCT​GAG​TCC​G-3′ 
GFP-VPS13B(VAB Δ R6)-F 5′-ACC​CTA​GTC​CTC​GAG​TGG​CCC​CAG​AAG​GAA​AAG​C-3′ 
GFP-VPS13B(VAB Δ R6)-R 5′-CAC​TCG​AGG​ACT​AGG​GTC​TTC​TGA​GAG​GGT​TA-3′ 
GFP-VPS13B(VAB Δ R2-5)-F 5′-TCA​TCT​GTG​ACC​CTA​GTC​CTC​GAG​TAA​TTA​TCC​A-3′ 
GFP-VPS13B(VAB Δ R2-5)-R 5′-ACT​AGG​GTC​ACA​GAT​GAT​AAT​CTG​TTT​TTG​TAC​TCC​A-3′ 
Sec23IP Δ 114–116AA-F 5′-CAA​CCT​CAG​TTC​TCC​CTT​TTA​CAA​CTG​GAT​CCC​A-3′ 
Sec23IP Δ l 114–116AA-R 5′-AAG​GGA​GAA​CTG​AGG​TTG​CTG​CAG​TTG​TTA​ATG-3′ 
Sec23IP Δ 176–178AA-F 5′-CCC​CAA​GGA​ATT​GGA​TAC​AAT​CCA​TAT​CGC​CAT​ACC-3′ 
Sec23IP Δ 176–178AA-R 5′-GTA​TCC​AAT​TCC​TTG​GGG​TTG​GTT​CCC​AAA​ATA-3′ 
Sec23IP Δ 207–209AA-F 5′-TGC​CAA​ACA​CCT​GCT​CAT​CCT​CCA​CCT​TCT​GGA-3′ 
Sec23IP Δ 207–209AA-R 5′-ATG​AGC​AGG​TGT​TTG​GCA​CTG​CTG​CAG​CTG​GGG-3′ 
Sec23IP Δ 213–215AA-F 5′-TCC​TGC​TCA​TTC​TGG​ACC​CCC​TGT​TCA​GAT​GTA-3′ 
Sec23IP Δ 213–215AA-R 5′-GGT​CCA​GAA​TGA​GCA​GGA​GGG​CCT​GGT​GTT​TGG-3′ 
Sec23IP Δ 232–234AA-F 5′-CTT​TGC​CAT​CTT​CAG​TGC​AGT​CAC​CGG​CAC​AGC-3′ 
Sec23IP Δ 232–234AA-R 5′-GCA​CTG​AAG​ATG​GCA​AAG​ATC​CTG​GAG​GCA​TCT-3′ 
Sec23IP Δ 257–259AA-F 5′-CTC​CCT​CTG​TTC​AAG​TGT​TTC​TAC​TTC​AAA​ACC​AAT​ATG​AGC​C-3′ 
Sec23IP Δ 257–259AA-R 5′-ACA​CTT​GAA​CAG​AGG​GAG​CCC​CAG​GTC​TGG​CAG-3′ 
Sec23IP Δ 305–307AA-F 5′-TTC​AGT​TCA​GGA​GAG​CGT​GGT​TCT​TGG​CAC​GGA-3′ 
Sec23IP Δ 305–307AA-R 5′-ACG​CTC​TCC​TGA​ACT​GAA​TTA​TAG​ATT​TCT​TCA​AGA​TT-3′ 
Halo-Sec23A-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​ACA​ACC​TAT​TTG​GAA​TTC​ATT​CA-3′ 
Halo-Sec23A-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​AGC​AGC​ACT​GGA​CAC​AGC-3′ 
Halo-Rab6A-F 5′-GAG​ATT​TCC​CTC​GAG​CTC​ATG​TCC​ACG​GGC​GGA​GAC​TTC-3′ 
Halo-Rab6A-R 5′-GTT​ATC​TAG​ATC​CGG​TGG​ATC​CTT​AGC​AGG​AAC​AGC​CTC-3′ 
Halo-Rab6A T27N-F 5′-GCG​TTG​GAA​AGA​ACT​CTT​TGA​TCA​CCA​GAT​TCA​TGT​ATG​A-3′ 
Halo-Rab6A T27N-R 5′-AGA​GTT​CTT​TCC​AAC​GCT​TTG​CTC​CCC​CAG​GA-3′ 
GFP-Rab1B-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​AAC​CCC​GAA​TAT​GAC​TAC​CTG-3′ 
GFP-Rab1B-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​GCA​ACA​GCC​ACC​GCC​A-3′ 
Halo-Rab1B-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​AAC​CCC​GAA​TAT​GAC​TAC​CTG-3′ 
Halo-Rab1B-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​GCA​ACA​GCC​ACC​GCC​A-3′ 
His-VPS13B-PH-F 5′-CTG​TAC​TTC​CAA​TCC​GCT​AGC​ACC​CTC​ACA​TCC​ATC​ACC​AAC​C-3′ 
His-VPS13B-PH-R 5′-TCC​TTT​CGG​GCT​TTG​GTC​GAC​TCA​AGG​AAA​CCC​TTT​CCT​CAG​G-3′ 
PrimersSequences (5′-3′)
Vector-VPS13B-F 5′-TCG​AGC​TCA​AGC​TTC​GAA​TTC​GCC​ACC​ATG​CTG​GAG​TCA​TAT​G-3′ 
VPS13B(1265AA)-R 5′-CCT​TTG​CTC​ATA​ACT​GGA​GAA​GTA​GGA​ACA​GGC​C-3′ 
VPS13B(1265AA)^sfGFP-F 5′-TCT​CCA​GTT​ATG​AGC​AAA​GGA​GAA​GAA​CTT​TTC​A-3′ 
SfGFP^VPS13B(1266AA)-R 5′-TGC​CTA​TAC​TGC​TTC​TTT​TGT​AGA​GCT​CAT​CCA​TG-3′ 
VPS13B(1266AA)-F 5′-AGA​AGC​AGT​ATA​GGC​ACA​GCT​CC-3′ 
Vector-VPS13B-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​AGG​AAA​CCC​TTT​CCT​CAG​G-3′ 
GFP-VPS13B-NT(1–1,500)-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​CTG​GAG​TCA​TAT​GTA​ACT​CCA​A-3′ 
GFP-VPS13B-NT(1–1,500)-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​ATC​AAA​AGC​TTG​TGC​TGA​AA-3′ 
GFP-VPS13B-N,S560D-F 5′-GAC​TTT​TCT​GAT​GGG​AAA​AGT​GAA​GAT​TTG​GGA​A-3′ 
GFP-VPS13B-N,S560D-R 5′-TTC​CCA​TCA​GAA​AAG​TCT​TGT​TGA​TTT​GTG​GAA​C-3′ 
GFP-VPS13B-N,S503D,P504A-F 5′-GAT​TAC​CGA​GAT​GCC​GAA​AAT​AAT​GGT​ACT​CGC​GCA​GA-3′ 
GFP-VPS13B-N,S503D,P504A-R 5′-TTC​GGC​ATC​TCG​GTA​ATC​AAA​CAA​TGA​ATT​TGT​AA-3′ 
GFP-VPS13B-N,S1403D-F 5′-CTT​CCT​TGA​CTG​TAC​TGA​CAA​GCT​GAA​CAG​ACG​C-3′ 
GFP-VPS13B-N,S1403D-R 5′-CAG​TAC​AGT​CAA​GGA​AGA​CAC​CTC​CAC​ATT​GC-3′ 
GFP-VPS13B-N,S14033D-F 5′-GCT​TCT​TTC​CTG​ACA​CAA​CTA​CAA​AAC​TTC​TAG​ATG​GCA​CTC-3′ 
GFP-VPS13B-N,S14033D-R 5′-TGT​GTC​AGG​AAA​GAA​GCC​AGA​GCA​ATT​ACT​GA-3′ 
Halo-VAPB-F 5′-GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCG​AAG​GTG​GAG​CAG​G-3′ 
Halo-VAPB-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​CAA​GGC​AAT​CTT​CCC​AAT​AAT​T-3′ 
Halo-MOSPD2-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCA​GAG​AAT​CAC​GCC​C-3′ 
Halo-MOSPD2-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​ACT​GTA​CAA​TAA​ATA​GAA​GAA​AGA​GG-3′ 
MGAT2-Halo-F 5′-TGA​ACC​GTC​AGA​TCC​GCT​AGC​GCC​ACC​ATG​AGG​TTC​CGC​ATC​TAC​AAA​CG-3′ 
MGAT2-Halo-R 5′-CGA​CTG​CAG​AAT​TCG​AAG​CTT​CTG​CAG​TCT​TCT​ATA​ACT​TTT​ACA​GAG​TTC-3′ 
GFP-Sec31A-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​AAG​TTA​AAG​GAA​GTA​GAT​CGT​ACA​GC-3′ 
GFP-Sec31A-R 5′-GCC​TGA​GTA​ATT​AAA​CCA​TCA​ATG​TCC​CCA​CTG​A-3′ 
mCherry-Sec31A-F 5′-CTG​CAG​TCG​ACG​GTA​CCG​CGG​ATG​AAG​TTA​AAG​GAA​GTA​GAT​CGT​ACA​GC-3′ 
mCherry-Sec31A-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GAC​ACC​CAG​CTT​ATT​GGC​C-3′ 
GFP-Sec23IP-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
GFP-Sec23IP-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
Halo-Sec23IP-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
Halo-Sec23IP-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
GST-Sec23IP-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​ATG​GCC​GAG​AGA​AAA​CCT​AAC​G-3′ 
GST-Sec23IP-R 5′-TCA​GTC​AGT​CAC​GAT​GAA​TTC​TCA​ATG​CTG​GGG​CTG​TTC​TG-3′ 
GST-Sec23IP NT-R 5′-CGA​TGA​ATT​CTC​ACT​CTA​GTT​TTT​CAC​TGA​ACT​CCT​CAG​TAT-3′ 
GST-Sec23IP ΔNT-F 5′-GAT​CTG​GTT​CCG​CGT​GGA​TCC​GCT​GAA​TAT​AAA​AAA​GCT​GTA​ACC​ACT-3′ 
Halo-Sec23IP NT-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​CTC​TAG​TTT​TTC​ACT​GAA​CTC​CTC​AG-3′ 
Halo-Sec23IP ΔNT-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​GCT​GAA​TAT​AAA​AAA​GCT​GTA​ACC​ACT-3′ 
Halo-Sec23IP(Δ 368–642)-F 5′-AGA​TCT​CGA​GCT​CAA​GCT​TCG​ATG​ACA​GTC​GTA​GGG​AAC​CCT​CG-3′ 
Halo-Sec23IP Δ 368–642)-R 5′-AAA​ACT​AGA​GAA​TAA​AGA​AGT​CCT​AAC​TTT-3′ 
Halo-Sec23IP(Δ SAM)-F 5′-GTT​GAA​AAG​AAG​GCA​GCG​TCA​GAA​AAG​AAG​GCA-3′ 
Halo-Sec23IP Δ SAM)-R 5′-CGC​TGC​CTT​CTT​TTC​AAC​AAC​AAG​GTC​ATA​CGA​CTC​A-3′ 
Halo-Sec23IP(Δ 708–779AA)-F 5′-AGC​CAA​ACT​GGA​TTT​TGA​ACC​AGA​GAT​ATT-3′ 
Halo-Sec23IP(Δ 708–779AA)-R 5′-GTT​CAA​AAT​CCA​GTT​TGG​CTG​CTT​TAT​GTT-3′ 
Halo-Sec23IP Δ DDHD)-F 5′-GCT​TAC​AAC​TCA​TTA​ACA​ATG​AAC​ATT​AGT​CCA​GAA​CAG​CC-3′ 
Halo-Sec23IP(Δ DDHD)-R 5′-GTT​AAT​GAG​TTG​TAA​GCA​ACA​GAA​ACC​TGT​CC-3′ 
Halo-Sec23IP Δ 989–1000AA)-F 5′-TAT​CGA​TGA​GGA​TCC​ACC​GGA​TCT​AGA​TAA​CT-3′ 
Halo-Sec23IP Δ 989–1000AA)-FR 5′-GTG​GAT​CCT​CAT​CGA​TAA​ATT​TCT​TTA​AGT​AGT​AAC​AGA​GC-3′ 
GFP-VPS13B-VAB-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​GTA​GAG​GAG​TTG​GTC​TTC​AGC​CA-3′ 
GFP-VPS13B-VAB-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​TAA​CCG​AGC​AGG​TTT​TAA​TTC​AAA-3′ 
GFP-VPS13B-PH-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​TGC​TTG​CTG​CTG​ACA​TCA​GAA-3′ 
GFP-VPS13B-PH-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​TCA​CTT​TGT​GAT​GTA​GTC​CAC​CAG​T-3′ 
VPS13B(Δ PH)-GFP-F 5′-CAT​GAA​GGG​ACA​TCT​TGT​CAC​CTG​GCC​CCC​AG-3′ 
VPS13B(Δ PH)-GFP-R 5′-CAA​GAT​GTC​CCT​TCA​TGC​TCC​TGG​CCT​GAG​CC-3′ 
VPS13B G2729R-F 5′-CAT​CTG​TAG​AAG​ACA​GAT​CAT​CTG​TAG​TTA​CTT​GTC​TCA​A-3′ 
VPS13B G2729R-R 5′-TCT​GTC​TTC​TAC​AGA​TGA​TAA​TCT​GTT​TTT​GTA​CTC​CA-3′ 
VPS13B I2820T-F 5′-CCA​CTA​TTT​ATG​TCT​GGT​GCA​CAG​TTT​TGA​CT-3′ 
VPS13B I2820T-R 5′-CCA​GAC​ATA​AAT​AGT​GGA​ACT​GTT​TGA​AGA​TGG​CAC-3′ 
VPS13B Y2822C-F 5′-ATT​TGT​GTC​TGG​TGC​ACA​GTT​TTG​ACT​TTA​GA-3′ 
VPS13B Y2822C-R 5′-GTG​CAC​CAG​ACA​CAA​ATA​ATG​GAA​CTG​TTT​GAA​GAT​GGC-3′ 
VPS13B N2993S-F 5′-GCT​TAT​CTC​CGA​ATC​CAA​ATG​GGA​CCT​CTG​GC-3′ 
VPS13B N2993S-R 5′-TGG​ATT​CGG​AGA​TAA​GCA​GGG​CCC​AGG​GCA​GA-3′ 
VPS13B A3033E-F 5′-ACT​GGG​AAA​ATA​CAA​ACA​CTG​TGC​ACA​AGT​CAG-3′ 
VPS13B A3033E-R 5′-GTT​TGT​ATT​TTC​CCA​GTA​TAT​TCC​AAT​TTG​AAA​AGC-3′ 
VPS13B N3113Y-F 5′-CTA​CAA​TAC​ACC​ATA​TCA​AAT​ATT​TTA​TAA​ACC​AC-3′ 
VPS13B N3113Y-R 5′-GAT​ATG​GTG​TAT​TGT​AGA​TTA​TTG​TAG​TCT​TGT​CTT​CAA​TCT​GAA​T-3′ 
VPS13B S3142R-F 5′-CCA​GAC​AGG​GCT​ACT​TTT​AGC​ATT​TGC​CCA​GG-3′ 
VPS13B S3142R-R 5′-AAA​GTA​GCC​CTG​TCT​GGA​ACA​CGA​AAA​TAC​TCC-3′ 
VPS13B R3223W-F 5′-CGG​GAA​TTT​CTG​GGA​AAA​TGG​ATT​CTG​TAC​CAG​GGC-3′ 
VPS13B R3223W-R 5′-TTT​CCC​AGA​AAT​TCC​CGA​GGG​GTA​CTG​CCA​CA-3′ 
VPS13B S3328R-F 5′-AGT​GGA​GGG​ATG​CCA​TTG​ACA​TCA​ACA​GTC​AG-3′ 
VPS13B S3328R-R 5′-AAT​GGC​ATC​CCT​CCA​CTC​AGT​TGT​TAC​TTC​ATC​TAG​AGG-3′ 
VPS13B G3432R-F 5′-TCC​CTA​GGG​AAG​AGC​CTG​TGG​CTG​CGT​TGT​TT-3′ 
VPS13B G3432R-R 5′-AGG​CTC​TTC​CCT​AGG​GAG​GGG​ACC​AGC​TCC​CG-3′ 
VPS13B V3470M-F 5′-TGT​CTT​AAT​GTG​CCA​GGG​AGA​AAA​AGC​AGA​AC-3′ 
VPS13B V3470M-R 5′-CCT​GGC​ACA​TTA​AGA​CAG​CAA​AGT​GGA​AAT​TGG​A-3′ 
GFP-VPS13B(VAB R1)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​TCT​TCA​GCC​ATT​TTG​TGA-3′ 
GFP-VPS13B(VAB R1)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​TCC​ACA​GAT​GAT​AAT​CTG​TTT​TTG​T-3′ 
GFP-VPS13B(VAB R2)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGC​AGA​TCA​TCT​GTA​GTT​ACT​TGT​CTC-3′ 
GFP-VPS13B(VAB R2)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GCT​GAA​CAC​AAT​CAT​TCG​TTG-3′ 
GFP-VPS13B(VAB R3)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGC​TTT​TTA​TCA​TGA​GGA​GTC​ATC-3′ 
GFP-VPS13B(VAB R3)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​CAG​AAG​TTC​TAT​CAA​CAA​AGT​TCT​AAC​A-3′ 
GFP-VPS13B(VAB R4)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​CCC​TGC​TTA​TCA​ATG​AA-3′ 
GFP-VPS13B(VAB R4)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​GTC​TTC​AAT​CTG​AAT​AAT​TTG​TAT​ACC​TT-3′ 
GFP-VPS13B(VAB R5)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGA​CTA​CAA​TAA​TCA​ATA​ATA​CAC​CAT​ATC​A-3′ 
GFP-VPS13B(VAB R5)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​TTC​TGA​GAG​GGT​TAA​ATA​AGT​CAC​TC-3′ 
GFP-VPS13B(VAB R6)-F 5′-TCA​GAT​CTC​GAG​CTC​AAG​CTT​CGG​TAA​TTA​TCC​ACA​ATA​GAT​GTC​CA-3′ 
GFP-VPS13B(VAB R6)-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TTA​AGT​GAT​GAA​GAC​TGT​GCC​ACA-3′ 
GFP-VPS13B(VAB Δ R1)-F 5′-AGG​AGT​TGA​GAC​AGA​TCA​TCT​GTA​GTT​ACT​TGT​CTC​AA-3′ 
GFP-VPS13B(VAB Δ R1)-R 5′-GAT​CTG​TCT​CAA​CTC​CTC​TAC​AGA​TCT​GAG​TCC​G-3′ 
GFP-VPS13B(VAB Δ R6)-F 5′-ACC​CTA​GTC​CTC​GAG​TGG​CCC​CAG​AAG​GAA​AAG​C-3′ 
GFP-VPS13B(VAB Δ R6)-R 5′-CAC​TCG​AGG​ACT​AGG​GTC​TTC​TGA​GAG​GGT​TA-3′ 
GFP-VPS13B(VAB Δ R2-5)-F 5′-TCA​TCT​GTG​ACC​CTA​GTC​CTC​GAG​TAA​TTA​TCC​A-3′ 
GFP-VPS13B(VAB Δ R2-5)-R 5′-ACT​AGG​GTC​ACA​GAT​GAT​AAT​CTG​TTT​TTG​TAC​TCC​A-3′ 
Sec23IP Δ 114–116AA-F 5′-CAA​CCT​CAG​TTC​TCC​CTT​TTA​CAA​CTG​GAT​CCC​A-3′ 
Sec23IP Δ l 114–116AA-R 5′-AAG​GGA​GAA​CTG​AGG​TTG​CTG​CAG​TTG​TTA​ATG-3′ 
Sec23IP Δ 176–178AA-F 5′-CCC​CAA​GGA​ATT​GGA​TAC​AAT​CCA​TAT​CGC​CAT​ACC-3′ 
Sec23IP Δ 176–178AA-R 5′-GTA​TCC​AAT​TCC​TTG​GGG​TTG​GTT​CCC​AAA​ATA-3′ 
Sec23IP Δ 207–209AA-F 5′-TGC​CAA​ACA​CCT​GCT​CAT​CCT​CCA​CCT​TCT​GGA-3′ 
Sec23IP Δ 207–209AA-R 5′-ATG​AGC​AGG​TGT​TTG​GCA​CTG​CTG​CAG​CTG​GGG-3′ 
Sec23IP Δ 213–215AA-F 5′-TCC​TGC​TCA​TTC​TGG​ACC​CCC​TGT​TCA​GAT​GTA-3′ 
Sec23IP Δ 213–215AA-R 5′-GGT​CCA​GAA​TGA​GCA​GGA​GGG​CCT​GGT​GTT​TGG-3′ 
Sec23IP Δ 232–234AA-F 5′-CTT​TGC​CAT​CTT​CAG​TGC​AGT​CAC​CGG​CAC​AGC-3′ 
Sec23IP Δ 232–234AA-R 5′-GCA​CTG​AAG​ATG​GCA​AAG​ATC​CTG​GAG​GCA​TCT-3′ 
Sec23IP Δ 257–259AA-F 5′-CTC​CCT​CTG​TTC​AAG​TGT​TTC​TAC​TTC​AAA​ACC​AAT​ATG​AGC​C-3′ 
Sec23IP Δ 257–259AA-R 5′-ACA​CTT​GAA​CAG​AGG​GAG​CCC​CAG​GTC​TGG​CAG-3′ 
Sec23IP Δ 305–307AA-F 5′-TTC​AGT​TCA​GGA​GAG​CGT​GGT​TCT​TGG​CAC​GGA-3′ 
Sec23IP Δ 305–307AA-R 5′-ACG​CTC​TCC​TGA​ACT​GAA​TTA​TAG​ATT​TCT​TCA​AGA​TT-3′ 
Halo-Sec23A-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​ACA​ACC​TAT​TTG​GAA​TTC​ATT​CA-3′ 
Halo-Sec23A-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​TCA​AGC​AGC​ACT​GGA​CAC​AGC-3′ 
Halo-Rab6A-F 5′-GAG​ATT​TCC​CTC​GAG​CTC​ATG​TCC​ACG​GGC​GGA​GAC​TTC-3′ 
Halo-Rab6A-R 5′-GTT​ATC​TAG​ATC​CGG​TGG​ATC​CTT​AGC​AGG​AAC​AGC​CTC-3′ 
Halo-Rab6A T27N-F 5′-GCG​TTG​GAA​AGA​ACT​CTT​TGA​TCA​CCA​GAT​TCA​TGT​ATG​A-3′ 
Halo-Rab6A T27N-R 5′-AGA​GTT​CTT​TCC​AAC​GCT​TTG​CTC​CCC​CAG​GA-3′ 
GFP-Rab1B-F 5′-TAC​AAG​TCC​GGA​CTC​AGA​TCT​ATG​AAC​CCC​GAA​TAT​GAC​TAC​CTG-3′ 
GFP-Rab1B-R 5′-CAG​AAT​TCG​AAG​CTT​GAG​CTC​CTA​GCA​ACA​GCC​ACC​GCC​A-3′ 
Halo-Rab1B-F 5′-CTG​GAG​ATT​TCC​CTC​GAG​CTC​ATG​AAC​CCC​GAA​TAT​GAC​TAC​CTG-3′ 
Halo-Rab1B-R 5′-TTA​TCT​AGA​TCC​GGT​GGA​TCC​CTA​GCA​ACA​GCC​ACC​GCC​A-3′ 
His-VPS13B-PH-F 5′-CTG​TAC​TTC​CAA​TCC​GCT​AGC​ACC​CTC​ACA​TCC​ATC​ACC​AAC​C-3′ 
His-VPS13B-PH-R 5′-TCC​TTT​CGG​GCT​TTG​GTC​GAC​TCA​AGG​AAA​CCC​TTT​CCT​CAG​G-3′ 
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