Table 1.

Reagent and siRNA used in this study

Reagent or resourceSourceIdentifier
Antibodies 
GFP antibody CST 2555 
Anti-Halo tag pAB Promega G928A 
VAPA rabbit pAb ABclonal A12939 
VAPB rabbit pAb ABclonal A5363 
MOSPD3 antibody Santa Cruz sc-514923 
His Tag antibody, mAb, mouse Genscript A00186 
Purified mouse anti-GM130 BD Biosciences 610822 
Anti-VPS13B antibody produced in rabbit Sigma-Aldrich HPA043865 
GM130 (D6B1) XP rabbit mAb CST 12480 
TGN46/TGOLN2 rabbit mAb ABclona A19618 
Sec31A antibody Santa Cruz sc-376587 
Sec31A polyclonal antibody Proteintech 17913-1-AP 
Sec23IP polyclonal antibody Proteintech 20892-1-AP 
Anti-GFP abcam ab6556 
COL1A1 DSHB SP1.D8 
GST-Tag Sinobiological 11213-MM01 
ERGIC-53 antibody Santa Cruz sc-271517 
COPA antibody (H-3) Santa Cruz sc-398099 
anti-alpha tubulin abcam Ab52866 
Purified anti-PtdIns(4)P IgM Echelon Z-P004 
RAB2A monoclonal antibody Proteintech 67501-1-Ig 
RAB6A polyclonal antibody Proteintech 10187-2-AP 
Beta actin monoclonal antibody Proteintech 66009-1-Ig 
HRP-conjugated Goat anti-mouse IgG Sangon Biotech D110087 
HRP-conjugated Goat anti-Rabbit IgG Sangon Biotech D110058 
Reagents 
PAO Sigma-Aldrich P3075 
Y294002 Sigma-Aldrich 440202 
Vps34-IN-1 MCE HY-12795 
Wortmannin MCE HY-10197 
BFA MCE HY-16592 
L-ascorbic acid MCE HY-B0166 
Hoechst 33342 Invitrogen H1399 
Briain PI(4)P Avanti 840045P 
DMSO Sigma-Aldrich D2650 
siRNA 
siRNA scrambled
5′-CGTTAATCGCGTATAATACGCGTAT-3′ 		Ribobio N/A 
siRNA human VPS13B#1:
5′-GACCTTACTTGTCATAATA-3′ 		  
siRNA human VPS13B#2:
5′-GCCTATGTTTATTCGTATA-3′ 		Ribobio N/A 
siRNA human VPS13B#3:
5′-GAAGGATCCTATTGTTTCT-3′ 		Ribobio N/A 
siRNA human RAB6A#1:
5′-GACATCTTTGATCACCAGA-3′ 		Ribobio N/A 
siRNA human RAB6A#2:
5′-TTACATCCGTGATTCTGCT-3′ 		Ribobio N/A 
siRNA human RAB6A#3:
5′-CAGCTGTAGTAGTTTACGA-3′ 		Ribobio N/A 
siRNA human RAB2A#1:
5′-GAAGGAGTCTTTGACATTA-3′ 		Ribobio N/A 
siRNA human RAB2A#2:
5′-GCAGGAGCTTTACTAGTTT-3′ 		Ribobio N/A 
siRNA human RAB2A#3:
5′-GTTCGGTGCTCGAATGATA-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#1:
5′-CCAGCTTAGTGAGAAGCAT-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#2:
5′-GCACCATCGGAACTGCTAA-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#3:
5′-CCATGAACTGAGAATTCAT-3′ 		Ribobio N/A 
siRNA human SEC23IP#1:
5′-GGCTAATCCTTACATTGCA-3′ 		Ribobio N/A 
siRNA human SEC23IP#2:
5′-TGGGAATATTGGACAGTCA-3′ 		Ribobio N/A 
siRNA human SEC23IP#3:
5′-CAGGGCTAATCCTTACATT-3′ 		Ribobio N/A 
siRNA human PI4KA:
5′-CCGCCATGTTCTCAGATAA-3′ 		Ribobio N/A 
siRNA human PI4KB:
5′-TGTTGGGGCTTCCCTGCCC-3′ 		Ribobio N/A 
siRNA house mouse Sec23IP:
5′-AGAAGATTGATATGGAGTCTC-3′ 		Ribobio N/A 
siRNA house mouse Sar1a#1:
5′-AGAAGAACTGACAATTGCTGG-3′ 		Ribobio N/A 
siRNA house mouse Sar1a#2:
5′-GCTGAAAGATGACAGACTAGG-3′ 		Ribobio N/A 
siRNA house mouse Tango1#1:
5′-GAGAAGCTGCTAACTTAAGGC-3′ 		Ribobio N/A 
siRNA house mouse Tango1#2:
5′-ACATACAAGACCTGATCTATT-3′ 		Ribobio N/A 
siRNA human ERGIC53:
5′-GGACAGAATCGTATTCATC-3′ 		Ribobio N/A 
Reagent or resourceSourceIdentifier
Antibodies 
GFP antibody CST 2555 
Anti-Halo tag pAB Promega G928A 
VAPA rabbit pAb ABclonal A12939 
VAPB rabbit pAb ABclonal A5363 
MOSPD3 antibody Santa Cruz sc-514923 
His Tag antibody, mAb, mouse Genscript A00186 
Purified mouse anti-GM130 BD Biosciences 610822 
Anti-VPS13B antibody produced in rabbit Sigma-Aldrich HPA043865 
GM130 (D6B1) XP rabbit mAb CST 12480 
TGN46/TGOLN2 rabbit mAb ABclona A19618 
Sec31A antibody Santa Cruz sc-376587 
Sec31A polyclonal antibody Proteintech 17913-1-AP 
Sec23IP polyclonal antibody Proteintech 20892-1-AP 
Anti-GFP abcam ab6556 
COL1A1 DSHB SP1.D8 
GST-Tag Sinobiological 11213-MM01 
ERGIC-53 antibody Santa Cruz sc-271517 
COPA antibody (H-3) Santa Cruz sc-398099 
anti-alpha tubulin abcam Ab52866 
Purified anti-PtdIns(4)P IgM Echelon Z-P004 
RAB2A monoclonal antibody Proteintech 67501-1-Ig 
RAB6A polyclonal antibody Proteintech 10187-2-AP 
Beta actin monoclonal antibody Proteintech 66009-1-Ig 
HRP-conjugated Goat anti-mouse IgG Sangon Biotech D110087 
HRP-conjugated Goat anti-Rabbit IgG Sangon Biotech D110058 
Reagents 
PAO Sigma-Aldrich P3075 
Y294002 Sigma-Aldrich 440202 
Vps34-IN-1 MCE HY-12795 
Wortmannin MCE HY-10197 
BFA MCE HY-16592 
L-ascorbic acid MCE HY-B0166 
Hoechst 33342 Invitrogen H1399 
Briain PI(4)P Avanti 840045P 
DMSO Sigma-Aldrich D2650 
siRNA 
siRNA scrambled
5′-CGTTAATCGCGTATAATACGCGTAT-3′ 		Ribobio N/A 
siRNA human VPS13B#1:
5′-GACCTTACTTGTCATAATA-3′ 		  
siRNA human VPS13B#2:
5′-GCCTATGTTTATTCGTATA-3′ 		Ribobio N/A 
siRNA human VPS13B#3:
5′-GAAGGATCCTATTGTTTCT-3′ 		Ribobio N/A 
siRNA human RAB6A#1:
5′-GACATCTTTGATCACCAGA-3′ 		Ribobio N/A 
siRNA human RAB6A#2:
5′-TTACATCCGTGATTCTGCT-3′ 		Ribobio N/A 
siRNA human RAB6A#3:
5′-CAGCTGTAGTAGTTTACGA-3′ 		Ribobio N/A 
siRNA human RAB2A#1:
5′-GAAGGAGTCTTTGACATTA-3′ 		Ribobio N/A 
siRNA human RAB2A#2:
5′-GCAGGAGCTTTACTAGTTT-3′ 		Ribobio N/A 
siRNA human RAB2A#3:
5′-GTTCGGTGCTCGAATGATA-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#1:
5′-CCAGCTTAGTGAGAAGCAT-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#2:
5′-GCACCATCGGAACTGCTAA-3′ 		Ribobio N/A 
siRNA house mouse Vps13b#3:
5′-CCATGAACTGAGAATTCAT-3′ 		Ribobio N/A 
siRNA human SEC23IP#1:
5′-GGCTAATCCTTACATTGCA-3′ 		Ribobio N/A 
siRNA human SEC23IP#2:
5′-TGGGAATATTGGACAGTCA-3′ 		Ribobio N/A 
siRNA human SEC23IP#3:
5′-CAGGGCTAATCCTTACATT-3′ 		Ribobio N/A 
siRNA human PI4KA:
5′-CCGCCATGTTCTCAGATAA-3′ 		Ribobio N/A 
siRNA human PI4KB:
5′-TGTTGGGGCTTCCCTGCCC-3′ 		Ribobio N/A 
siRNA house mouse Sec23IP:
5′-AGAAGATTGATATGGAGTCTC-3′ 		Ribobio N/A 
siRNA house mouse Sar1a#1:
5′-AGAAGAACTGACAATTGCTGG-3′ 		Ribobio N/A 
siRNA house mouse Sar1a#2:
5′-GCTGAAAGATGACAGACTAGG-3′ 		Ribobio N/A 
siRNA house mouse Tango1#1:
5′-GAGAAGCTGCTAACTTAAGGC-3′ 		Ribobio N/A 
siRNA house mouse Tango1#2:
5′-ACATACAAGACCTGATCTATT-3′ 		Ribobio N/A 
siRNA human ERGIC53:
5′-GGACAGAATCGTATTCATC-3′ 		Ribobio N/A 
View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal