Table I.

Final list of genes with actin phenotypes conserved between Drosophila and mammalian cells

FBgn  Gene  Phenotype group  Known actin regulator  Validated  Function  Mammalian orthologue(s)  HeLa actinome hit(s)  Mouse nuclear hit(s)  
FBgn0015610  Caf1  High actin  No  Yes  Chromatin remodeling  RBBP4  No  N/A  
FBgn0034577  cpa  High actin  Yes  Yes  F-actin capping  CAPZA1, CAPZA2, CAPZA3  CAPZA1, CAPZA3  N/A  
FBgn0011570  cpb  High actin  Yes  Yes  F-actin capping  CAPZB  CAPZB  N/A  
FBgn0000042  Act5C  Low actin  Yes  Yes  Actin filament formation  ACTG1  ACTG1  N/A  
FBgn0000308  chic  Low actin  Yes  Yes  G-actin binding  PFN1, PFN2, PFN3, PFN4  PFN1, PFN4 (PFN3 not screened)  N/A  
FBgn0011785  BRWD3  Other actin  No  Yes  WD40 domain protein  BRWD1,BRWD3, PHIP  No  N/A  
FBgn0028388  Capt  Other actin  Yes  Yes  G-actin binding  CAP1, CAP2  No  N/A  
FBgn0035586  CG10671  Other actin  No  No  Endoplasmic reticulum  FITM1, FITM2  No  N/A  
FBgn0039205  CG13623  Other actin  No  Yes  Mitochondrial  ISCA2 (HBLD1)  ISCA2  N/A  
FBgn0001491  L(1)10Bb  Other actin  No  Yes  GPCR signaling  BUD31 (G10)  BUD31  N/A  
FBgn0044826  Pak3  Other actin  Yes  Yes  Rac GTPase signaling  PAK3  No  N/A  
FBgn0021967  Pdsw  Other actin  No  No  Mitochondial electron transport chain  NDUFB10  No  N/A  
FBgn0011726  tsr  Other actin  Yes  Yes  F-actin severing  CFL1, CFL2, DSTN  CFL1, DSTN  N/A  
FBgn0052138  CG32138  Spiky  Moderately  Yes  Uncharacterized formin family member  FMNL1, FMNL2, FMNL3  FMNL1  N/A  
FBgn0002183  dre4  Spiky  No  Yes  DNA repair  SUPT16H  SUPT16H  N/A  
FBgn0015509  lin19  Spiky  No  Yes  Ubiquitin-dependent protein degradation  CUL1  Not screened  N/A  
FBgn0040291  Roc1b  Spiky  No  Yes  Ubiquitin-dependent protein degradation  RBX1  RBX1  N/A  
FBgn0003415  skd  Spiky  No  Yes  Mediator complex  MED13, MED13L  No  N/A  
FBgn0005411  U2af50  Spiky  No  Yes  RNA splicing  U2AF2  U2AF2  N/A  
FBgn0020510  Abi  Spiky (SCAR-like)  Yes  Yes  SCAR complex  ABI1, ABI2  No  N/A  
FBgn0031781  Arc-p20a  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC4  ARPC4  N/A  
FBgn0011742  Arp14D  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ACTR2  No  N/A  
FBgn0011744  Arp66B  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ACTR3  No  N/A  
FBgn0039754  CG9747  Spiky (SCAR-like)  No  Yes  Fatty acid desaturase  SCD, SCD5, TRMT2A, TRMT2B  SCD (only one screened)  N/A  
FBgn0011771  Hem  Spiky (SCAR-like)  Yes  Yes  SCAR complex  NCKAP1  NCKAP1  N/A  
FBgn0031437  p16-arc  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC5  No  N/A  
FBgn0010333  Rac1  Spiky (SCAR-like)  Yes  Yes  Rho family GTPase  RAC1  RAC1  N/A  
FBgn0014011  Rac2  Spiky (SCAR-like)  Yes  Yes  Rho family GTPase  RAC2  No  N/A  
FBgn0041781  SCAR  Spiky (SCAR-like)  Yes  Yes  SCAR complex  WASF1, WASF2, WASF3  WASF3  N/A  
FBgn0016984  Sktl  Spiky (SCAR-like)  Yes  Yes  Lipid kinase  PIPK51A, PIPK51B, PIPK51C  PIP5K1C  N/A  
FBgn0023423  slmb  Spiky (SCAR-like)  No  Yes  Ubiquitin-dependent protein degradation  BTRC, FBXW11  Not screened  N/A  
FBgn0038320  Sra-1  Spiky (SCAR-like)  Yes  Yes  SCAR complex  CYFIP1, CYFIP2  CYFIP1  N/A  
FBgn0032859  Arc-p34  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC2  ARPC2  N/A  
FBgn0038369  Arpc3a  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC3A  No  N/A  
FBgn0065032  Arpc3b  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC3B  No  N/A  
FBgn0001961  Sop2  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC1A, ARPC1B  ARPC1A  N/A  
FBgn0033260  Cul-4  Spiky (SCAR-like)b  No  Yes  ubiquitin-dependent protein degradation  CUL4A, CUL4B  Not screened  N/A  
FBgn0025638  Roc1a  Spiky (SCAR-like)b  No  Yes  ubiquitin-dependent protein degradation  RBX1  RBX1  N/A  
FBgn0022213  Cas  Nuclear actin  No  Yes  Nuclear transport  Cse1l  N/A  No  
FBgn0030121  CG17446  Nuclear actin  No  Yes  Transcription  Cxxc1  N/A  No  
FBgn0031492  CG3542  Nuclear actin  No  No  Splicing  Prpf40a, Prpf40b  N/A  N/A  
FBgn0037093  CG7597  Nuclear actin  No  Yes  Splicing (kinase)  Crkrs, Cdc2l5  N/A  Cdc2l5 (Crkrs not tested)  
FBgn0001337  Exp6  Nuclear actin  Yes  Yes  Nuclear transport  Xpo6  N/A  Yes  
FBgn0037657  hyx  Nuclear actin  No  Yes  Transcription  Cdc73  N/A  Yes  
FBgn0016696  Pitslre  Nuclear actin  No  No  Splicing (kinase)  Cdc2l1  N/A  N/A  
FBgn0003449  snf  Nuclear actin  No  Yes  Splicing  Snrpa, Snrpb2  N/A  Too toxic to assess  
FBgn  Gene  Phenotype group  Known actin regulator  Validated  Function  Mammalian orthologue(s)  HeLa actinome hit(s)  Mouse nuclear hit(s)  
FBgn0015610  Caf1  High actin  No  Yes  Chromatin remodeling  RBBP4  No  N/A  
FBgn0034577  cpa  High actin  Yes  Yes  F-actin capping  CAPZA1, CAPZA2, CAPZA3  CAPZA1, CAPZA3  N/A  
FBgn0011570  cpb  High actin  Yes  Yes  F-actin capping  CAPZB  CAPZB  N/A  
FBgn0000042  Act5C  Low actin  Yes  Yes  Actin filament formation  ACTG1  ACTG1  N/A  
FBgn0000308  chic  Low actin  Yes  Yes  G-actin binding  PFN1, PFN2, PFN3, PFN4  PFN1, PFN4 (PFN3 not screened)  N/A  
FBgn0011785  BRWD3  Other actin  No  Yes  WD40 domain protein  BRWD1,BRWD3, PHIP  No  N/A  
FBgn0028388  Capt  Other actin  Yes  Yes  G-actin binding  CAP1, CAP2  No  N/A  
FBgn0035586  CG10671  Other actin  No  No  Endoplasmic reticulum  FITM1, FITM2  No  N/A  
FBgn0039205  CG13623  Other actin  No  Yes  Mitochondrial  ISCA2 (HBLD1)  ISCA2  N/A  
FBgn0001491  L(1)10Bb  Other actin  No  Yes  GPCR signaling  BUD31 (G10)  BUD31  N/A  
FBgn0044826  Pak3  Other actin  Yes  Yes  Rac GTPase signaling  PAK3  No  N/A  
FBgn0021967  Pdsw  Other actin  No  No  Mitochondial electron transport chain  NDUFB10  No  N/A  
FBgn0011726  tsr  Other actin  Yes  Yes  F-actin severing  CFL1, CFL2, DSTN  CFL1, DSTN  N/A  
FBgn0052138  CG32138  Spiky  Moderately  Yes  Uncharacterized formin family member  FMNL1, FMNL2, FMNL3  FMNL1  N/A  
FBgn0002183  dre4  Spiky  No  Yes  DNA repair  SUPT16H  SUPT16H  N/A  
FBgn0015509  lin19  Spiky  No  Yes  Ubiquitin-dependent protein degradation  CUL1  Not screened  N/A  
FBgn0040291  Roc1b  Spiky  No  Yes  Ubiquitin-dependent protein degradation  RBX1  RBX1  N/A  
FBgn0003415  skd  Spiky  No  Yes  Mediator complex  MED13, MED13L  No  N/A  
FBgn0005411  U2af50  Spiky  No  Yes  RNA splicing  U2AF2  U2AF2  N/A  
FBgn0020510  Abi  Spiky (SCAR-like)  Yes  Yes  SCAR complex  ABI1, ABI2  No  N/A  
FBgn0031781  Arc-p20a  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC4  ARPC4  N/A  
FBgn0011742  Arp14D  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ACTR2  No  N/A  
FBgn0011744  Arp66B  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ACTR3  No  N/A  
FBgn0039754  CG9747  Spiky (SCAR-like)  No  Yes  Fatty acid desaturase  SCD, SCD5, TRMT2A, TRMT2B  SCD (only one screened)  N/A  
FBgn0011771  Hem  Spiky (SCAR-like)  Yes  Yes  SCAR complex  NCKAP1  NCKAP1  N/A  
FBgn0031437  p16-arc  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC5  No  N/A  
FBgn0010333  Rac1  Spiky (SCAR-like)  Yes  Yes  Rho family GTPase  RAC1  RAC1  N/A  
FBgn0014011  Rac2  Spiky (SCAR-like)  Yes  Yes  Rho family GTPase  RAC2  No  N/A  
FBgn0041781  SCAR  Spiky (SCAR-like)  Yes  Yes  SCAR complex  WASF1, WASF2, WASF3  WASF3  N/A  
FBgn0016984  Sktl  Spiky (SCAR-like)  Yes  Yes  Lipid kinase  PIPK51A, PIPK51B, PIPK51C  PIP5K1C  N/A  
FBgn0023423  slmb  Spiky (SCAR-like)  No  Yes  Ubiquitin-dependent protein degradation  BTRC, FBXW11  Not screened  N/A  
FBgn0038320  Sra-1  Spiky (SCAR-like)  Yes  Yes  SCAR complex  CYFIP1, CYFIP2  CYFIP1  N/A  
FBgn0032859  Arc-p34  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC2  ARPC2  N/A  
FBgn0038369  Arpc3a  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC3A  No  N/A  
FBgn0065032  Arpc3b  Spiky (SCAR-like)a  Yes  Yes  ARP2/3 complex  ARPC3B  No  N/A  
FBgn0001961  Sop2  Spiky (SCAR-like)  Yes  Yes  ARP2/3 complex  ARPC1A, ARPC1B  ARPC1A  N/A  
FBgn0033260  Cul-4  Spiky (SCAR-like)b  No  Yes  ubiquitin-dependent protein degradation  CUL4A, CUL4B  Not screened  N/A  
FBgn0025638  Roc1a  Spiky (SCAR-like)b  No  Yes  ubiquitin-dependent protein degradation  RBX1  RBX1  N/A  
FBgn0022213  Cas  Nuclear actin  No  Yes  Nuclear transport  Cse1l  N/A  No  
FBgn0030121  CG17446  Nuclear actin  No  Yes  Transcription  Cxxc1  N/A  No  
FBgn0031492  CG3542  Nuclear actin  No  No  Splicing  Prpf40a, Prpf40b  N/A  N/A  
FBgn0037093  CG7597  Nuclear actin  No  Yes  Splicing (kinase)  Crkrs, Cdc2l5  N/A  Cdc2l5 (Crkrs not tested)  
FBgn0001337  Exp6  Nuclear actin  Yes  Yes  Nuclear transport  Xpo6  N/A  Yes  
FBgn0037657  hyx  Nuclear actin  No  Yes  Transcription  Cdc73  N/A  Yes  
FBgn0016696  Pitslre  Nuclear actin  No  No  Splicing (kinase)  Cdc2l1  N/A  N/A  
FBgn0003449  snf  Nuclear actin  No  Yes  Splicing  Snrpa, Snrpb2  N/A  Too toxic to assess  
a

Spiky (SCAR-like) annotation added after re-assessment with a longer RNAi period. See text for details.

b

Spiky (SCAR-like) annotation added after network analysis and reassessment. See text for details.

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