Antibodies and reagents
| Antibody or reagent . | Catalog numbers/Source . | Application and dilution . |
|---|---|---|
| Lipofectamine 2000 | Invitrogen | Transfection |
| Anti-Halo | G9281 (Promega) | WB 1:1,000 |
| Anti-DYNC1H1 (DHC) | 12345-1-AP (Proteintech) | WB 1:500 |
| Anti-p150[Glued] | 610473 (BD Biosciences) | WB 1:1,000 |
| Anti-UKHC F-5 (KHC) | Sc-133184 (Santa Cruz) | WB 1:1,000 |
| Anti-BICD2 | Ab117818 (Abcam) | WB 1:1,000 |
| Anti-Nesprin2 | PA5-78438 (Invitrogen) | WB 1:1,000; IHC and ICC 1:200 |
| HRP-conjugated anti-β-actin | Sc-47778 (Santa Cruz) | WB 1:1,000 |
| Anti-p27-Kip1 | 610241 (BD Biosciences) | IHC 1:400 |
| Anti-Ki-67 | 14–5698-82 (eBioscience) | IHC 1:1,000 |
| Anti-laminB1 | Ab16048 (Abcam) | ICC 1:1,000 |
| Anti-γ-tubulin | T5192 (Sigma-Aldrich) | ICC 1:250 |
| Anti- β-tubulin | T4026 (Sigma-Aldrich) | ICC 1:1,000 |
| Anti-acetylated-tubulin (6-11B-1) | T6793 (Sigma-Aldrich) | ICC 1:1,000 |
| SiR-DNA | CY-SC007 (Cytoskeleton) | Live-cell imaging 200 nM |
| Antibody or reagent . | Catalog numbers/Source . | Application and dilution . |
|---|---|---|
| Lipofectamine 2000 | Invitrogen | Transfection |
| Anti-Halo | G9281 (Promega) | WB 1:1,000 |
| Anti-DYNC1H1 (DHC) | 12345-1-AP (Proteintech) | WB 1:500 |
| Anti-p150[Glued] | 610473 (BD Biosciences) | WB 1:1,000 |
| Anti-UKHC F-5 (KHC) | Sc-133184 (Santa Cruz) | WB 1:1,000 |
| Anti-BICD2 | Ab117818 (Abcam) | WB 1:1,000 |
| Anti-Nesprin2 | PA5-78438 (Invitrogen) | WB 1:1,000; IHC and ICC 1:200 |
| HRP-conjugated anti-β-actin | Sc-47778 (Santa Cruz) | WB 1:1,000 |
| Anti-p27-Kip1 | 610241 (BD Biosciences) | IHC 1:400 |
| Anti-Ki-67 | 14–5698-82 (eBioscience) | IHC 1:1,000 |
| Anti-laminB1 | Ab16048 (Abcam) | ICC 1:1,000 |
| Anti-γ-tubulin | T5192 (Sigma-Aldrich) | ICC 1:250 |
| Anti- β-tubulin | T4026 (Sigma-Aldrich) | ICC 1:1,000 |
| Anti-acetylated-tubulin (6-11B-1) | T6793 (Sigma-Aldrich) | ICC 1:1,000 |
| SiR-DNA | CY-SC007 (Cytoskeleton) | Live-cell imaging 200 nM |