Table 1.

Primer list

NameSequence
Svf1 S1 5′-TGC​ATC​GAA​TCA​AGA​AAA​AAG​AAA​CGA​ATA​AGC​TTT​TGT​ATA​ATA​TAA​TAT​AAT​GCG​TAC​GCT​GCA​GGT​GCA​C-3′ 
Svf1 S2 5′-GTA​GTA​TTA​AAT​CAC​AGA​TAG​GAA​AAA​GCA​TAC​GAG​ACC​TTC​CCA​CTT​TTG​CTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1 S3 5′-CAT​TCA​TTT​CTG​AAT​CAG​ATG​TGA​TTA​GTG​AGG​AGT​CTT​ACA​ATG​AAG​CAC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Svf1_pom_n_GFP_fo 5′-AAT​CAA​GAA​AAA​AGA​AAC​GAA​TAA​GCT​TTT​GTA​TAA​TAT​AAT​ATA​ATG​TGC​AGG​TCG​ACA​ACC​CTT​AAT-3′ 
Svf1_pom_n_GFP_re 5′-AGC​CAT​ACC​AGT​GAC​TGC​TGA​GAT​CCC​ACC​TTT​TAT​CCA​CTT​CAA​GCG​GCC​GCA​TAG​GCC​ACT-3′ 
SUR2_S1 5′-TAA​CAT​TCT​AGT​CCG​AAG​AGG​GTG​TAT​ACG​AAA​AGA​AAA​TAT​ACG​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
SUR2_S2 5′-GAC​ATT​GCC​TTT​ACC​CAG​CAA​TTG​AAC​GGG​AGG​TAT​GCA​AAA​GGG​TTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Nvj2_S1 5′-CAT​CGA​AGA​GCA​GAA​CAG​CAA​GAG​AAA​AGT​AGC​ATT​AAA​AGA​CCA​TAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Nvj2_S2 5′-GCT​TCA​AGT​GAT​ATT​TAT​TTA​TTT​TTA​ATA​TAG​TAC​CGT​GGA​CTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Mak3_S1 5′-GAT​TAC​AAG​ATA​AAA​AAG​CCA​CTA​CTA​CAG​AAA​AGG​CGT​TGG​GTC​AGG​ACA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Mak3_S2 5′-GTA​TTA​TTA​ATA​TAT​ATT​TTA​TCA​TCA​TCG​AGT​GTT​TTT​CCT​TTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1_V12D_for 5′-ATC​TCA​GCA​GAC​ACT​GGT​ATG-3′ 
Svf1_V12D_rev 5′-CCC​ACC​TTT​TAT​CCA​CTT​C-3′ 
Ydr222w_S1 5′-GTG​AGG​GCG​TAT​AAG​ATA​CAT​CGT​ACA​TAC​ATA​GAG​ACT​CAT​TTA​GTG​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
Ydr222w_S2 5′-GAA​AAA​AAA​GGA​CAA​AAA​ACG​AAT​TTC​ATG​TGG​AAG​TGT​TCA​CGC​TTT​TGT​TCA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Ylr225c_S1 5′-GGG​CAA​CGA​ACA​GTT​TAA​TTA​ACA​TAA​AAA​TCA​TAG​AGC​AAA​GAA​AAA​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
Ylr225c_S2 5′-GAT​ATA​ATA​TTC​TTG​CAA​TGA​TGA​AAA​TAT​GTA​TAG​TCA​AAT​TTC​ATT​TTT​TTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1 G7AG8A Q5 for 5′-TGG​ATA​AAA​GCT​GCG​ATC​TCA​GCA​GTC-3′ 
Svf1 G7AG8A Q5 rev 5′-CTT​CAA​CAT​TAT​ATT​ATA​TTA​TAC​AAA​AGC-3′ 
Osh2_S1 5′-ATA​TTT​CAT​CGA​TAA​TCA​TAA​GCT​TAA​GCT​CGC​CAA​CTG​AAA​ACT​TCA​CAA​TAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh2_S2 5′-CAT​TCA​AAA​TAC​ATA​CAA​GTA​CCA​GGA​AAA​AAG​CTC​GCA​TAA​AAA​AGG​CGT​GTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Osh3_S1 5′-ATT​TGC​TGG​AGA​GTA​GTA​AAA​CGA​GCT​TGA​ATA​GCA​GCC​TGC​GAA​TCG​AAT​TAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh3_S2 5′-ATA​AAT​AGA​GTA​CAA​AGG​GGG​GAA​AAA​AGT​TCA​CTT​GAT​GTC​ATC​AAG​GCA​TTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Osh4_S1 5′-CCT​AGA​AAC​AAA​TCG​AAA​AAT​TTA​TAA​GAT​TTA​GTC​TCA​AGA​ATT​TCA​AGT​CAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh4_S2 5′-GAT​AGA​TAA​TAT​ATT​AGT​GCA​ACG​GTA​ACA​AGT​TGT​TAC​TTT​ATC​GTT​CTC​CTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Sec31 S2 5′-CAA​GGC​CAA​TAC​GCC​ACT​TTT​TGT​ACT​GAA​AGT​TTT​GAG​ACT​GAA​TTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Sec31 S3 5′-CTG​GCT​GAC​AGG​AGT​GAA​GAG​GTT​GAT​TGG​CAT​AGC​TGA​AGC​GAC​TTT​GAA​TCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Nvj2_pRS_KO_for 5′-GGT​TTT​GAA​CAC​ATC​GAA​GAG​CAG​AAC​AGC​AAG​AGA​AAA​GTA​GCA​TTA​AAA​GAC​CAG​ATT​GTA​CTG​AGA​GTG​CAC-3′ 
Nvj2_pRS_KO_rev 5′-GCA​TAT​AGC​TTC​AAG​TGA​TAT​TTA​TTT​ATT​TTT​AAT​ATA​GTA​CCG​TGG​ACC​TGT​GCG​GTA​TTT​CAC​ACC​G-3′ 
Q5_L2E_for 5′-TAA​TAT​AAT​GGA​GAA​GTG​GAT​AAA​AGG​TG-3′ 
Q5_L2E_rev 5′-TAT​TAT​ACA​AAA​GCT​TAT​TCG​TTT​C-3′ 
HAHA_for 5′-CAT​GAA​GCC​TGC​TGC​CGC​AGC​TAA​GG-3′ 
HAHA_rev 5′-CCT​TGG​AAG​GCC​ATT​ATA​AAT​G-3′ 
AUR1_S2 5′-ATA​TGT​ATC​TAC​ATA​AGA​CCA​ACC​GTA​TCC​GTA​ATT​GCA​GAT​AAA​ATA​CTC​ATT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
AUR1_S3 5′-TTC​TGT​TTC​TCG​TTC​GTC​CGC​CAC​GTC​TAT​AAC​GTC​ACT​AGG​TGT​AAA​GAG​GGC​TCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Svf1_19aa_for_Grh1_AH 5′-GGA​CTT​TTG​AGC​AGA​GCG​TGC​AAG​ATG​AAT​ATG​GGA​AAG​ATT​ACA​TTC​ATA​GTG-3′ 
Svf1_pr_rev_oh_Grh1AH 5′-GTA​CGA​GGT​TTT​TAG​CTA​TTC​TAA​ACA​TTA​TAT​TAT​ATT​ATA​CAA​AAG​CTT​ATT​CGT​TTC-3′ 
GFP_for_oh_Svf1 5′-AGT​CTT​ACA​ATG​AAG​CAC​GTA​CGC​TGC​AGG​TCG​ACG​G-3′ 
GFP_rev_oh_Svf1_t 5′-GAC​CTT​CCC​ACT​TTT​GCT​TAT​TTG​TAC​AAT​TCA​TCC​ATA​CC-3′ 
pRS405Svf1_term_f 5′-ATG​AAT​TGT​ACA​AAT​AAG​CAA​AAG​TGG​GAA​GGT​CTC​G-3′ 
pRS405Svf1_rev_oh 5′-TCG​ACC​TGC​AGC​GTA​CGT​GCT​TCA​TTG​TAA​GAC​TCC​TCA​C-3′ 
pRS405Svf1_AH_GFP_Q5mut_for 5′-CGT​ACG​CTG​CAG​GTC​GAC-3′ 
pRS405Svf1_AH_GFP_Q5mut_rev 5′-AGG​CTC​AGC​CAT​ACC​AGT​G-3′ 
Galpr_for_oh_pRS 5′-CCG​GGC​CCC​CCC​TCG​AGT​GAA​GTA​CGG​ATT​AGA​AGC​C-3′ 
Galpr_rev_oh_Svf1 5′-TTT​ATC​CAC​TTC​AAC​ATG​TTT​TTT​CTC​CTT​GAC​GTT​AAA​G-3′ 
Svf1_for_oh_Galpr 5′-CGT​CAA​GGA​GAA​AAA​ACA​TGT​TGA​AGT​GGA​TAA​AAG​GTG-3′ 
pRS_rev_oh_Galpr 5′-TTC​TAA​TCC​GTA​CTT​CAC​TCG​AGG​GGG​GGC​CCG​G-3′ 
Svf1_term_oh_3xFlag 5′-TAC​AAA​GAT​GAC​GAC​GAT​AAG​GAT​TAT​AAG​GAC​GAC​GAC​GAC​AAA​TAA​TCG​TAT​GCT​TTT-3′ 
Svf1_rev_3xFLAG_oh 5′-TCG​TCG​TCA​TCT​TTG​TAG​TCC​TTG​TCA​TCA​TCA​TCC​TTG​TAA​TCT​GCT​TCA​TTG​TAA​GAC-3′ 
Mnn9_S2 5′-TCT​TTC​AAT​AAC​GCT​ATA​GCT​TCT​GTA​TGC​TTT​TTG​CTC​AGT​TGC​TCA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Mnn9_S3 5′-TTT​GGC​TTA​CCA​AAC​TAT​TTG​GTT​TAT​CAC​ATA​GAG​GAA​GAG​AAC​CAT​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Sec63_S2 5′-ATA​CGT​CTA​AGA​GCT​AAA​ATG​AAA​AAC​TAT​ACT​AAT​CAC​TTA​TAT​CTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Sec63_S3 5′-ACT​GAT​ATC​GAT​ACG​GAT​ACA​GAA​GCT​GAA​GAT​GAT​GAA​TCA​CCA​GAA​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Sec7_S2 5′-TTC​TAC​AAC​TAA​GCA​TAT​TTT​AAT​CTG​CTG​GAC​CAT​TCA​ACA​AAG​CCT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3′ 
Sec7_S3 5′-CTA​TAA​AAC​AAT​TTC​TAA​GCA​GAG​TTG​GTG​AAT​TAT​ACC​TTT​CTA​CTG​ATC​GTA​CCG​CTG​CAG​GTC​GAC-3′ 
Vps4_S2 5′-TAT​TTT​CAT​GTA​CAC​AAG​AAA​TCT​ACA​TTA​GCA​CGT​TAA​TCA​ATT​GAC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3′ 
Vps4_S3 5′-GAC​TTG​CTG​AAG​CAA​GAA​CAG​TTC​ACT​AGA​GAT​TTT​GGT​CAA​GAA​GGT​AAC​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Lys2_S1 5′-GAA​AAA​CTG​CTA​ATT​ATA​GAG​AGA​TAT​CAC​AGA​GTT​ACT​CAC​TAA​TGC​GTA​CCG​CTG​CAG​GTC​GAC-3′ 
Lys2_S2 5′-CAT​ATT​TAA​TTA​TTG​TAC​ATG​GAC​ATA​TCA​TAC​GTA​ATG​CTC​AAC​CTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
NameSequence
Svf1 S1 5′-TGC​ATC​GAA​TCA​AGA​AAA​AAG​AAA​CGA​ATA​AGC​TTT​TGT​ATA​ATA​TAA​TAT​AAT​GCG​TAC​GCT​GCA​GGT​GCA​C-3′ 
Svf1 S2 5′-GTA​GTA​TTA​AAT​CAC​AGA​TAG​GAA​AAA​GCA​TAC​GAG​ACC​TTC​CCA​CTT​TTG​CTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1 S3 5′-CAT​TCA​TTT​CTG​AAT​CAG​ATG​TGA​TTA​GTG​AGG​AGT​CTT​ACA​ATG​AAG​CAC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Svf1_pom_n_GFP_fo 5′-AAT​CAA​GAA​AAA​AGA​AAC​GAA​TAA​GCT​TTT​GTA​TAA​TAT​AAT​ATA​ATG​TGC​AGG​TCG​ACA​ACC​CTT​AAT-3′ 
Svf1_pom_n_GFP_re 5′-AGC​CAT​ACC​AGT​GAC​TGC​TGA​GAT​CCC​ACC​TTT​TAT​CCA​CTT​CAA​GCG​GCC​GCA​TAG​GCC​ACT-3′ 
SUR2_S1 5′-TAA​CAT​TCT​AGT​CCG​AAG​AGG​GTG​TAT​ACG​AAA​AGA​AAA​TAT​ACG​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
SUR2_S2 5′-GAC​ATT​GCC​TTT​ACC​CAG​CAA​TTG​AAC​GGG​AGG​TAT​GCA​AAA​GGG​TTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Nvj2_S1 5′-CAT​CGA​AGA​GCA​GAA​CAG​CAA​GAG​AAA​AGT​AGC​ATT​AAA​AGA​CCA​TAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Nvj2_S2 5′-GCT​TCA​AGT​GAT​ATT​TAT​TTA​TTT​TTA​ATA​TAG​TAC​CGT​GGA​CTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Mak3_S1 5′-GAT​TAC​AAG​ATA​AAA​AAG​CCA​CTA​CTA​CAG​AAA​AGG​CGT​TGG​GTC​AGG​ACA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3′ 
Mak3_S2 5′-GTA​TTA​TTA​ATA​TAT​ATT​TTA​TCA​TCA​TCG​AGT​GTT​TTT​CCT​TTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1_V12D_for 5′-ATC​TCA​GCA​GAC​ACT​GGT​ATG-3′ 
Svf1_V12D_rev 5′-CCC​ACC​TTT​TAT​CCA​CTT​C-3′ 
Ydr222w_S1 5′-GTG​AGG​GCG​TAT​AAG​ATA​CAT​CGT​ACA​TAC​ATA​GAG​ACT​CAT​TTA​GTG​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
Ydr222w_S2 5′-GAA​AAA​AAA​GGA​CAA​AAA​ACG​AAT​TTC​ATG​TGG​AAG​TGT​TCA​CGC​TTT​TGT​TCA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Ylr225c_S1 5′-GGG​CAA​CGA​ACA​GTT​TAA​TTA​ACA​TAA​AAA​TCA​TAG​AGC​AAA​GAA​AAA​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3′ 
Ylr225c_S2 5′-GAT​ATA​ATA​TTC​TTG​CAA​TGA​TGA​AAA​TAT​GTA​TAG​TCA​AAT​TTC​ATT​TTT​TTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Svf1 G7AG8A Q5 for 5′-TGG​ATA​AAA​GCT​GCG​ATC​TCA​GCA​GTC-3′ 
Svf1 G7AG8A Q5 rev 5′-CTT​CAA​CAT​TAT​ATT​ATA​TTA​TAC​AAA​AGC-3′ 
Osh2_S1 5′-ATA​TTT​CAT​CGA​TAA​TCA​TAA​GCT​TAA​GCT​CGC​CAA​CTG​AAA​ACT​TCA​CAA​TAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh2_S2 5′-CAT​TCA​AAA​TAC​ATA​CAA​GTA​CCA​GGA​AAA​AAG​CTC​GCA​TAA​AAA​AGG​CGT​GTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Osh3_S1 5′-ATT​TGC​TGG​AGA​GTA​GTA​AAA​CGA​GCT​TGA​ATA​GCA​GCC​TGC​GAA​TCG​AAT​TAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh3_S2 5′-ATA​AAT​AGA​GTA​CAA​AGG​GGG​GAA​AAA​AGT​TCA​CTT​GAT​GTC​ATC​AAG​GCA​TTC​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Osh4_S1 5′-CCT​AGA​AAC​AAA​TCG​AAA​AAT​TTA​TAA​GAT​TTA​GTC​TCA​AGA​ATT​TCA​AGT​CAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Osh4_S2 5′-GAT​AGA​TAA​TAT​ATT​AGT​GCA​ACG​GTA​ACA​AGT​TGT​TAC​TTT​ATC​GTT​CTC​CTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
Sec31 S2 5′-CAA​GGC​CAA​TAC​GCC​ACT​TTT​TGT​ACT​GAA​AGT​TTT​GAG​ACT​GAA​TTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Sec31 S3 5′-CTG​GCT​GAC​AGG​AGT​GAA​GAG​GTT​GAT​TGG​CAT​AGC​TGA​AGC​GAC​TTT​GAA​TCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Nvj2_pRS_KO_for 5′-GGT​TTT​GAA​CAC​ATC​GAA​GAG​CAG​AAC​AGC​AAG​AGA​AAA​GTA​GCA​TTA​AAA​GAC​CAG​ATT​GTA​CTG​AGA​GTG​CAC-3′ 
Nvj2_pRS_KO_rev 5′-GCA​TAT​AGC​TTC​AAG​TGA​TAT​TTA​TTT​ATT​TTT​AAT​ATA​GTA​CCG​TGG​ACC​TGT​GCG​GTA​TTT​CAC​ACC​G-3′ 
Q5_L2E_for 5′-TAA​TAT​AAT​GGA​GAA​GTG​GAT​AAA​AGG​TG-3′ 
Q5_L2E_rev 5′-TAT​TAT​ACA​AAA​GCT​TAT​TCG​TTT​C-3′ 
HAHA_for 5′-CAT​GAA​GCC​TGC​TGC​CGC​AGC​TAA​GG-3′ 
HAHA_rev 5′-CCT​TGG​AAG​GCC​ATT​ATA​AAT​G-3′ 
AUR1_S2 5′-ATA​TGT​ATC​TAC​ATA​AGA​CCA​ACC​GTA​TCC​GTA​ATT​GCA​GAT​AAA​ATA​CTC​ATT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
AUR1_S3 5′-TTC​TGT​TTC​TCG​TTC​GTC​CGC​CAC​GTC​TAT​AAC​GTC​ACT​AGG​TGT​AAA​GAG​GGC​TCG​TAC​GCT​GCA​GGT​CGA​C-3′ 
Svf1_19aa_for_Grh1_AH 5′-GGA​CTT​TTG​AGC​AGA​GCG​TGC​AAG​ATG​AAT​ATG​GGA​AAG​ATT​ACA​TTC​ATA​GTG-3′ 
Svf1_pr_rev_oh_Grh1AH 5′-GTA​CGA​GGT​TTT​TAG​CTA​TTC​TAA​ACA​TTA​TAT​TAT​ATT​ATA​CAA​AAG​CTT​ATT​CGT​TTC-3′ 
GFP_for_oh_Svf1 5′-AGT​CTT​ACA​ATG​AAG​CAC​GTA​CGC​TGC​AGG​TCG​ACG​G-3′ 
GFP_rev_oh_Svf1_t 5′-GAC​CTT​CCC​ACT​TTT​GCT​TAT​TTG​TAC​AAT​TCA​TCC​ATA​CC-3′ 
pRS405Svf1_term_f 5′-ATG​AAT​TGT​ACA​AAT​AAG​CAA​AAG​TGG​GAA​GGT​CTC​G-3′ 
pRS405Svf1_rev_oh 5′-TCG​ACC​TGC​AGC​GTA​CGT​GCT​TCA​TTG​TAA​GAC​TCC​TCA​C-3′ 
pRS405Svf1_AH_GFP_Q5mut_for 5′-CGT​ACG​CTG​CAG​GTC​GAC-3′ 
pRS405Svf1_AH_GFP_Q5mut_rev 5′-AGG​CTC​AGC​CAT​ACC​AGT​G-3′ 
Galpr_for_oh_pRS 5′-CCG​GGC​CCC​CCC​TCG​AGT​GAA​GTA​CGG​ATT​AGA​AGC​C-3′ 
Galpr_rev_oh_Svf1 5′-TTT​ATC​CAC​TTC​AAC​ATG​TTT​TTT​CTC​CTT​GAC​GTT​AAA​G-3′ 
Svf1_for_oh_Galpr 5′-CGT​CAA​GGA​GAA​AAA​ACA​TGT​TGA​AGT​GGA​TAA​AAG​GTG-3′ 
pRS_rev_oh_Galpr 5′-TTC​TAA​TCC​GTA​CTT​CAC​TCG​AGG​GGG​GGC​CCG​G-3′ 
Svf1_term_oh_3xFlag 5′-TAC​AAA​GAT​GAC​GAC​GAT​AAG​GAT​TAT​AAG​GAC​GAC​GAC​GAC​AAA​TAA​TCG​TAT​GCT​TTT-3′ 
Svf1_rev_3xFLAG_oh 5′-TCG​TCG​TCA​TCT​TTG​TAG​TCC​TTG​TCA​TCA​TCA​TCC​TTG​TAA​TCT​GCT​TCA​TTG​TAA​GAC-3′ 
Mnn9_S2 5′-TCT​TTC​AAT​AAC​GCT​ATA​GCT​TCT​GTA​TGC​TTT​TTG​CTC​AGT​TGC​TCA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Mnn9_S3 5′-TTT​GGC​TTA​CCA​AAC​TAT​TTG​GTT​TAT​CAC​ATA​GAG​GAA​GAG​AAC​CAT​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Sec63_S2 5′-ATA​CGT​CTA​AGA​GCT​AAA​ATG​AAA​AAC​TAT​ACT​AAT​CAC​TTA​TAT​CTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3′ 
Sec63_S3 5′-ACT​GAT​ATC​GAT​ACG​GAT​ACA​GAA​GCT​GAA​GAT​GAT​GAA​TCA​CCA​GAA​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Sec7_S2 5′-TTC​TAC​AAC​TAA​GCA​TAT​TTT​AAT​CTG​CTG​GAC​CAT​TCA​ACA​AAG​CCT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3′ 
Sec7_S3 5′-CTA​TAA​AAC​AAT​TTC​TAA​GCA​GAG​TTG​GTG​AAT​TAT​ACC​TTT​CTA​CTG​ATC​GTA​CCG​CTG​CAG​GTC​GAC-3′ 
Vps4_S2 5′-TAT​TTT​CAT​GTA​CAC​AAG​AAA​TCT​ACA​TTA​GCA​CGT​TAA​TCA​ATT​GAC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3′ 
Vps4_S3 5′-GAC​TTG​CTG​AAG​CAA​GAA​CAG​TTC​ACT​AGA​GAT​TTT​GGT​CAA​GAA​GGT​AAC​CGT​ACC​GCT​GCA​GGT​CGA​C-3′ 
Lys2_S1 5′-GAA​AAA​CTG​CTA​ATT​ATA​GAG​AGA​TAT​CAC​AGA​GTT​ACT​CAC​TAA​TGC​GTA​CCG​CTG​CAG​GTC​GAC-3′ 
Lys2_S2 5′-CAT​ATT​TAA​TTA​TTG​TAC​ATG​GAC​ATA​TCA​TAC​GTA​ATG​CTC​AAC​CTT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3′ 
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