Table 4.

List of all the solutions used in the project

SolutionComposition
HL3 0.3 mM Ca2++ NaCl 70 mM, KCl 5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 115 mM, Ca2+ 0.3 mM, pH 7.2 
HL3 1 mM Ca2+ NaCl 70 mM, KCl 5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 115 mM, Ca2+ 1 mM, pH 7.2 
HL3 high K+ NaCl 40 mM, KCl 90 mM, Ca2+ 1.5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 5 mM, pH 7.2 
10x PBS 1.37 M NaCl, 27 mM KCl, 100 mM Na2HPO4, 20 mM KH2PO4, pH 7.4 
PBSTx PBS 1x, 0.3% Triton X (vol/vol) 
20x SSC 20 g NaCl, 100.5 g Tri-Sodium Citrate, pH 7.0 in 1 liter 
TE 10 mM Tris-HCL, 1 mM EDTA, pH 8.0 
SolutionComposition
HL3 0.3 mM Ca2++ NaCl 70 mM, KCl 5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 115 mM, Ca2+ 0.3 mM, pH 7.2 
HL3 1 mM Ca2+ NaCl 70 mM, KCl 5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 115 mM, Ca2+ 1 mM, pH 7.2 
HL3 high K+ NaCl 40 mM, KCl 90 mM, Ca2+ 1.5 mM, MgCl2 20 mM, NaHCO3 10 mM, trehalose 5 mM, HEPES 5 mM, sucrose 5 mM, pH 7.2 
10x PBS 1.37 M NaCl, 27 mM KCl, 100 mM Na2HPO4, 20 mM KH2PO4, pH 7.4 
PBSTx PBS 1x, 0.3% Triton X (vol/vol) 
20x SSC 20 g NaCl, 100.5 g Tri-Sodium Citrate, pH 7.0 in 1 liter 
TE 10 mM Tris-HCL, 1 mM EDTA, pH 8.0 
[ViewLarge]
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal