List of D. melanogaster lines utilized in this study
| Line name . | Line ID . | Line source . | Line description . |
|---|---|---|---|
| nrv2::YFP | CPTI001455 | Kyoto Stock Centre - Drosophila Genomics and Genetic Resources (DGRC) (https://www.dgrc.kit.ac.jp/) | Nervana 2 gene YFP protein trap line (Lowe et al., 2014) |
| Flo-2::YFP | CPTI001427 | DGRC | Flotillin 2 gene YFP protein trap line |
| Cip4::YFP | CPTI003231 | DGRC | Cdc42-interacting protein 4 gene YFP protein trap line |
| Vha55::YFP | CPTI002645 | DGRC | Vacuolar H+-ATPase 55kD subunit gene YFP protein trap line |
| Atpalpha::YFP | CPTI002636 | DGRC | Na pump α subunit gene YFP protein trap line |
| Nrg::YFP | CPTI002761 | DGRC | Neuroglian gene YFP protein trap line |
| Lac::YFP | CPTI001714 | DGRC | Lachesin gene YFP protein trap line |
| alpha-Cat::YFP | CPTI002408 | DGRC | α Catenin gene YFP protein trap line |
| Pdi::YFP | CPTI002342 | DGRC | Protein disulfide isomerase gene YFP protein trap line |
| gs2::YFP | CPTI001918 | DGRC | Glutamine synthetase 2 gene YFP protein trap line |
| Repo-GAL4 | RRID:BDSC_7415 | Bloomington Drosophila Stock Center (BDSC) (https://bdsc.indiana.edu/index.html) | Expresses GAL4 in glia |
| UAS-mCD8-mCherry/cyo-GFP | RRID:BDSC_27391 | BDSC | Expresses Cherry RFP fused to the mouse CD8 extracellular and transmembrane domains for membrane targeting under UAS control |
| UAS-mCD8-GFP/cyo-GFP | RRID:BDSC 63045 | BDSC | Expresses GFP fused to the mouse CD8 extracellular and transmembrane domains for membrane targeting under UAS control |
| Cyo-GFP/Gla; tm6, tb/pri | Crossed from BDSC stocks | BDSC | A double balanced stock carrying Cyo-GFP over gla on the 2nd chromosome and tm6, tb over prickle on the 3rd chromosome |
| Atpalpha-RNAi | RRID:BDSC 32913 | BDSC | Expresses dsRNA for RNAi of atpalpha under UAS control in the VALIUM20 vector |
| alpha-Cat-RNAi | KK107298 | VDRC | Expresses dsRNA for RNAi of alpha-Cat under UAS control in the pUAST vector pMF3 |
| nrv2-RNAi | GD960 | VDRC | Expresses dsRNA for RNAi of nrv2 under UAS control in the pUAST vector pMF3 |
| shot-RNAi | RRID:BDSC 28336 | BDSC | Expresses dsRNA for RNAi of shot under UAS control in the VALIUM10 vector |
| Vha55-RNAi | GD 9363 | VDRC | Expresses dsRNA for RNAi of Vha55 under UAS control in the pUAST vector pMF3 |
| Lac-RNAi | GD 35524 | VDRC | Expresses dsRNA for RNAi of Lac under UAS control in the pUAST vector pMF3 |
| Flo-2-RNAi | VSH 330316 | VDRC | Expresses dsRNA for RNAi of Flo-2 under UAS control in the WALIUM20 vector |
| gs2-RNAi | GD 9378 | VDRC | Expresses dsRNA for RNAi of gs2 under UAS control in the pUAST vector pMF3 |
| Pdi-RNAi | GD13418 | VDRC | Expresses dsRNA for RNAi of Pdi under UAS control in the pUAST vector pMF3 |
| mCherry-RNAi | RRID:BDSC 35785 | BDSC | Expresses dsRNA for RNAi of mCherry under UAS control in the VALIUM20 vector |
| nrv2-GAL4 | RRID: BDSC 6800 | BDSC | Expresses GAL4 in wrapping glia |
| 46F-GALl4 | − | Gift from Dr. Stefanie Schirmeier | Expresses GAL4 in perineurial glia (Xie and Auld, 2011) |
| Mdr65-GAL4 | RRID: BDSC 50472 | BDSC | Expresses GAL4 in subperineurial glia |
| Line name . | Line ID . | Line source . | Line description . |
|---|---|---|---|
| nrv2::YFP | CPTI001455 | Kyoto Stock Centre - Drosophila Genomics and Genetic Resources (DGRC) (https://www.dgrc.kit.ac.jp/) | Nervana 2 gene YFP protein trap line (Lowe et al., 2014) |
| Flo-2::YFP | CPTI001427 | DGRC | Flotillin 2 gene YFP protein trap line |
| Cip4::YFP | CPTI003231 | DGRC | Cdc42-interacting protein 4 gene YFP protein trap line |
| Vha55::YFP | CPTI002645 | DGRC | Vacuolar H+-ATPase 55kD subunit gene YFP protein trap line |
| Atpalpha::YFP | CPTI002636 | DGRC | Na pump α subunit gene YFP protein trap line |
| Nrg::YFP | CPTI002761 | DGRC | Neuroglian gene YFP protein trap line |
| Lac::YFP | CPTI001714 | DGRC | Lachesin gene YFP protein trap line |
| alpha-Cat::YFP | CPTI002408 | DGRC | α Catenin gene YFP protein trap line |
| Pdi::YFP | CPTI002342 | DGRC | Protein disulfide isomerase gene YFP protein trap line |
| gs2::YFP | CPTI001918 | DGRC | Glutamine synthetase 2 gene YFP protein trap line |
| Repo-GAL4 | RRID:BDSC_7415 | Bloomington Drosophila Stock Center (BDSC) (https://bdsc.indiana.edu/index.html) | Expresses GAL4 in glia |
| UAS-mCD8-mCherry/cyo-GFP | RRID:BDSC_27391 | BDSC | Expresses Cherry RFP fused to the mouse CD8 extracellular and transmembrane domains for membrane targeting under UAS control |
| UAS-mCD8-GFP/cyo-GFP | RRID:BDSC 63045 | BDSC | Expresses GFP fused to the mouse CD8 extracellular and transmembrane domains for membrane targeting under UAS control |
| Cyo-GFP/Gla; tm6, tb/pri | Crossed from BDSC stocks | BDSC | A double balanced stock carrying Cyo-GFP over gla on the 2nd chromosome and tm6, tb over prickle on the 3rd chromosome |
| Atpalpha-RNAi | RRID:BDSC 32913 | BDSC | Expresses dsRNA for RNAi of atpalpha under UAS control in the VALIUM20 vector |
| alpha-Cat-RNAi | KK107298 | VDRC | Expresses dsRNA for RNAi of alpha-Cat under UAS control in the pUAST vector pMF3 |
| nrv2-RNAi | GD960 | VDRC | Expresses dsRNA for RNAi of nrv2 under UAS control in the pUAST vector pMF3 |
| shot-RNAi | RRID:BDSC 28336 | BDSC | Expresses dsRNA for RNAi of shot under UAS control in the VALIUM10 vector |
| Vha55-RNAi | GD 9363 | VDRC | Expresses dsRNA for RNAi of Vha55 under UAS control in the pUAST vector pMF3 |
| Lac-RNAi | GD 35524 | VDRC | Expresses dsRNA for RNAi of Lac under UAS control in the pUAST vector pMF3 |
| Flo-2-RNAi | VSH 330316 | VDRC | Expresses dsRNA for RNAi of Flo-2 under UAS control in the WALIUM20 vector |
| gs2-RNAi | GD 9378 | VDRC | Expresses dsRNA for RNAi of gs2 under UAS control in the pUAST vector pMF3 |
| Pdi-RNAi | GD13418 | VDRC | Expresses dsRNA for RNAi of Pdi under UAS control in the pUAST vector pMF3 |
| mCherry-RNAi | RRID:BDSC 35785 | BDSC | Expresses dsRNA for RNAi of mCherry under UAS control in the VALIUM20 vector |
| nrv2-GAL4 | RRID: BDSC 6800 | BDSC | Expresses GAL4 in wrapping glia |
| 46F-GALl4 | − | Gift from Dr. Stefanie Schirmeier | Expresses GAL4 in perineurial glia (Xie and Auld, 2011) |
| Mdr65-GAL4 | RRID: BDSC 50472 | BDSC | Expresses GAL4 in subperineurial glia |