Table 3. Comparison of Boltzmann fit... | Rockefeller University Press

Table 3.

Comparison of Boltzmann fit parameters for spHCN^R332C fluorescence before and after exposure to 50 mM KI

	ALEXA-488 (9)		MTS-TAMRA (11)
	100K	100K+KI	100K	100K+KI
$∆ F_{t o t a l}^{\max}$	1.00 ± 0.02	0.74 ± 0.03^{^a}	1.01 ± 0.02	0.95 ± 0.03
V_0.5^F (mV)	−75.3 ± 1.2	−73.8 ± 2.3	−61.5 ± 2.1	−73.7 ± 2.6^{^a}
z^F	1.06 ± 0.05	1.05 ± 0.09	0.89 ± 0.05	0.77 ± 0.04

	ALEXA-488 (9)		MTS-TAMRA (11)
	100K	100K+KI	100K	100K+KI
$∆ F_{t o t a l}^{\max}$	1.00 ± 0.02	0.74 ± 0.03^{^a}	1.01 ± 0.02	0.95 ± 0.03
V_0.5^F (mV)	−75.3 ± 1.2	−73.8 ± 2.3	−61.5 ± 2.1	−73.7 ± 2.6^{^a}
z^F	1.06 ± 0.05	1.05 ± 0.09	0.89 ± 0.05	0.77 ± 0.04

Values shown as mean ± SE as reported by fit to pooled, normalized data using Boltzmann equation. Number of cells analyzed given in parentheses. Data sets with iodide were normalized to ${∆ F}_{t o t a l}^{\max}$ for the control condition for each cell before pooling. Shaded values after iodide exposure.

Indicates significant difference relative to control for each fluorophore (t test, P < 0.05).

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