Drosophila stocks used in study
| Stocks . | Sources . |
|---|---|
| Protein expression by native promoters | |
| Arp3-GFP | Xie et al. (2021) |
| Jupiter-GFP | Bloomington Drosophila stock center (BDSC) #6836 |
| Rab5-YFP | BDSC #62543 |
| Rab8-YFP | BDSC #62546 |
| Rab11-YFP | BDSC #62549 |
| Spider-GFP | BDSC #59025 |
| SCAR-NG | Dobramysl et al. (2021) |
| Spg-NG | This paper |
| Tubulin-GFP | Andrew Wilde, University of Toronto, Toronto, Canada |
| Zip-GFP | BDSC #51564 |
| Protein expression by non-native promoters | |
| Sqh-Gap43-mCherry | Martin et al. (2009) |
| Sqh-Moesin-actin binding domain-GFP | Kiehart et al. (2000) |
| Sqh-Pak3-Rac-GTP binding domain-GFP | BDSC #52304 |
| Tubulin-Steppke-PH domain-GFP (tGPH) | BDSC #8163 |
| Ubi-PLCγ-PH domain-mCherry | Herszterg et al. (2013) |
| Maternal-GAL4-VP16 | Mark Peifer, University of North Carolina at Chapel Hill, USA |
| UAS-driven protein expression | |
| UASp-Arf1-GFP | Shao et al. (2010) |
| UASp-Rab8-GFP | BDSC #9782 |
| UASp-RFP-KDEL | BDSC #30910 |
| UASp-Sec15-GFP | BDSC #39685 |
| UAS-driven shRNA expression | |
| UASp-Arp3 shRNA | BDSC #53972 |
| UASp-ASAP shRNA (ineffective shRNA—control used in combination with mCherry probes) | Rodrigues et al. (2016) |
| UASp-cnn shRNA | BDSC #57149 |
| UASp-mCherry shRNA (control) | BDSC #35785 |
| UASp-zip shRNA | BDSC #36727 |
| Stocks . | Sources . |
|---|---|
| Protein expression by native promoters | |
| Arp3-GFP | Xie et al. (2021) |
| Jupiter-GFP | Bloomington Drosophila stock center (BDSC) #6836 |
| Rab5-YFP | BDSC #62543 |
| Rab8-YFP | BDSC #62546 |
| Rab11-YFP | BDSC #62549 |
| Spider-GFP | BDSC #59025 |
| SCAR-NG | Dobramysl et al. (2021) |
| Spg-NG | This paper |
| Tubulin-GFP | Andrew Wilde, University of Toronto, Toronto, Canada |
| Zip-GFP | BDSC #51564 |
| Protein expression by non-native promoters | |
| Sqh-Gap43-mCherry | Martin et al. (2009) |
| Sqh-Moesin-actin binding domain-GFP | Kiehart et al. (2000) |
| Sqh-Pak3-Rac-GTP binding domain-GFP | BDSC #52304 |
| Tubulin-Steppke-PH domain-GFP (tGPH) | BDSC #8163 |
| Ubi-PLCγ-PH domain-mCherry | Herszterg et al. (2013) |
| Maternal-GAL4-VP16 | Mark Peifer, University of North Carolina at Chapel Hill, USA |
| UAS-driven protein expression | |
| UASp-Arf1-GFP | Shao et al. (2010) |
| UASp-Rab8-GFP | BDSC #9782 |
| UASp-RFP-KDEL | BDSC #30910 |
| UASp-Sec15-GFP | BDSC #39685 |
| UAS-driven shRNA expression | |
| UASp-Arp3 shRNA | BDSC #53972 |
| UASp-ASAP shRNA (ineffective shRNA—control used in combination with mCherry probes) | Rodrigues et al. (2016) |
| UASp-cnn shRNA | BDSC #57149 |
| UASp-mCherry shRNA (control) | BDSC #35785 |
| UASp-zip shRNA | BDSC #36727 |