gPLC and fPLC biosensor activity
| . | . | gPLC . | . | fPLC . | ||||
|---|---|---|---|---|---|---|---|---|
| . | PI(3,4,5)P3 to PI(4,5)P2 . | PI(4,5)P2 to PI(4)P . | ||||||
| . | n . | Pulse time (s) . | V1/2 . | Slope . | n . | Pulse time (s) . | V1/2 . | Slope . |
| WT | 20 | 1.5 | 90 ± 3 | 28 ± 2 | 27 | 2 | 92 ± 3 | 26 ± 3 |
| F127A | 8 | 1.5 | 137 ± 6 | 36 ± 3 | 8 | 2 | 104 ± 5 | 28 ± 4 |
| I131A | 9 | 1.5 | 104 ± 7 | 44 ± 5 | 8 | 2 | 125 ± 6 | 33 ± 4 |
| I134A | 9 | 1.5 | 89 ± 2 | 20 ± 1 | 14 | 2 | 81 ± 2 | 18 ± 2 |
| L137A | 8 | 3 | 64 ± 7 | 50 ± 8 | 9 | 2 | 147 ± 14 | 23 ± 8 |
| S1-Q | 9 | 3 | 167 ± 20 | 58 ± 7 | 11 | 2 | 181 ± 91 | 15 ± 22 |
| WT | N/A | N/A | N/A | N/A | 11 | 20 | 70.2 ± 0.7 | 18.0 ± 0.7 |
| S1-Q | N/A | N/A | N/A | N/A | 9 | 20 | 119 ± 13 | 44 ± 8 |
| . | . | gPLC . | . | fPLC . | ||||
|---|---|---|---|---|---|---|---|---|
| . | PI(3,4,5)P3 to PI(4,5)P2 . | PI(4,5)P2 to PI(4)P . | ||||||
| . | n . | Pulse time (s) . | V1/2 . | Slope . | n . | Pulse time (s) . | V1/2 . | Slope . |
| WT | 20 | 1.5 | 90 ± 3 | 28 ± 2 | 27 | 2 | 92 ± 3 | 26 ± 3 |
| F127A | 8 | 1.5 | 137 ± 6 | 36 ± 3 | 8 | 2 | 104 ± 5 | 28 ± 4 |
| I131A | 9 | 1.5 | 104 ± 7 | 44 ± 5 | 8 | 2 | 125 ± 6 | 33 ± 4 |
| I134A | 9 | 1.5 | 89 ± 2 | 20 ± 1 | 14 | 2 | 81 ± 2 | 18 ± 2 |
| L137A | 8 | 3 | 64 ± 7 | 50 ± 8 | 9 | 2 | 147 ± 14 | 23 ± 8 |
| S1-Q | 9 | 3 | 167 ± 20 | 58 ± 7 | 11 | 2 | 181 ± 91 | 15 ± 22 |
| WT | N/A | N/A | N/A | N/A | 11 | 20 | 70.2 ± 0.7 | 18.0 ± 0.7 |
| S1-Q | N/A | N/A | N/A | N/A | 9 | 20 | 119 ± 13 | 44 ± 8 |