Key resources table
| Reagent . | Source . | Identifier . |
|---|---|---|
| pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) | Addgene | RRID: Addgene_55209 |
| XL10 Ultracompetent E. coli | Agilent Technologies | Cat: 200314 |
| pMAL-c5E | Addgene | RRID: Addgene_161783 |
| pmCherryC1 | Clontech | Cat: 632524, ID: PT3975-5 |
| pCDH | SBI | Cat: CD500B-1, RRID: Addgene_72265 |
| BL21 star (DE3) E. coli | Agilent Technologies | Cat: C601003 |
| HeLa S3 cells | ATCC | ATCC Cat: CCL-2.2, RRID: CVCL_0058 |
| HEK 293T | UC Berkeley Cell Culture Facility | RRID: CVCL_0063 |
| pSpCas9(BB)-2A-GFP vector | Addgene | RRID: Addgene_48138 |
| pMRX-IP-LC3-Halo | Addgene | RRID: Addgene_184899 |
| pMRX-IP-Parkin | Addgene | RRID: Addgene_38248 |
| pMRX-IB-Su9-GFP-Halo | Addgene | RRID: Addgene_184905 |
| pmCherryC1-Rubicon | Addgene | RRID: Addgene_216697 |
| pmCherryC1-Pacer | Addgene | RRID: Addgene_216698 |
| pmCherryC1-Pacer K534N, R623T | Addgene | RRID: Addgene_216699 |
| 2CT-PacerRH | Addgene | RRID: Addgene_216700 |
| 2CT-PacerRH K534N | Addgene | RRID: Addgene_216701 |
| 2CT-PacerRH R623T | Addgene | RRID: Addgene_216702 |
| 2CT-PacerRH R628K | Addgene | RRID: Addgene_216703 |
| 2CT-PacerRH K534N, R623T | Addgene | RRID: Addgene_216704 |
| 2CT-PacerRH K534N,R623T,R628K | Addgene | RRID: Addgene_216705 |
| pMAL-c5e-Rubicon RH | Addgene | RRID: Addgene_216706 |
| pMAL-c5e-Rubicon RH N821K, T911R, K916R | Addgene | RRID: Addgene_216707 |
| 2GT-RAB7A Q67L | Addgene | RRID: Addgene_216708 |
| pCAG-TSF-TBK1 | Addgene | RRID: Addgene_216709 |
| pCG-VSV-G | Addgene | RRID: Addgene_8454 |
| pCMVR8.74 | Addgene | RRID: Addgene_22036 |
| Halo-WIPI2B | Addgene | RRID: Addgene_175025 |
| pCIG2-IRES-Lamp1-mNeon Green | Addgene | RRID: Addgene_219441 |
| pHTN-LC3B | Addgene | RRID: Addgene_219440 |
| pS72 Rab7 rabbit monoclonal | Abcam | Cat: ab302494, RRID: AB_2933985 |
| Anti Rab7 rabbit monoclonal antibody | Abcam | Cat: ab137029, RRID: AB_2629474 |
| Anti Pacer rabbit antibody | Millipore Sigma | Cat: HPA026614, RRID: AB_10602693 |
| Rubicon (D9F7) rabbit monoclonal antibody | Cell Signaling | Cat: #8465, RRID: AB_10891617 |
| Anti-alpha tubulin antibody [DM1A]—Loading Control | Abcam | Cat: ab7291, RRID: AB_2241126 |
| Goat anti-rabbit IgG (H + L) highly Cross-adsorbed secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat: A11034, RRID: AB_2576217 |
| Goat anti-rabbit IgG H&L (HRP) | Abcam | Cat: ab6721, RRID:AB_955447 |
| HSP60 rabbit polyclonal antibody | Sigma-Aldrich | Cat# SAB4501464, RRID: AB_10746162 |
| Anti mCherry chicken polyclonal antibody | Abcam | Cat: ab205402, RRID: AB_2722769 |
| HeLa S3 RUBCN KO clone 15 | This paper | AC: CVCL_D5AG |
| S3 HeLas RUBCN KO + HA-Parkin/pSu9-HaloTag7-mGFP | This paper | AC: CVCL_D5AJ |
| HeLa S3 RUBCN KO/HaloTag7-LC3 | This paper | AC: CVCL_D5AK |
| HeLa S3 RUBCNL KO clone 17 (Pacer KO) | This paper | AC: CVCL_D5AH |
| HeLa S3 RUBCNL KO (Pacer KO)/HA-PRKN/pSu9-HaloTag7-mGFP | This paper | AC: CVCL_D5AL |
| HeLa S3 RUBCNL KO (Pacer KO)/HaloTag7-LC3 | This paper | AC: CVCL_D5AM |
| HeLa S3 PRKN | This paper | AC: CVCL_D5AN |
| HeLa S3 HA-PRKN/HaloTag7-mGFP | This paper | AC: CVCL_D5AP |
| HeLa S3 HaloTag7-LC3 | This paper | AC: CVCL_D5AQ |
| Reagent . | Source . | Identifier . |
|---|---|---|
| pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10) | Addgene | RRID: Addgene_55209 |
| XL10 Ultracompetent E. coli | Agilent Technologies | Cat: 200314 |
| pMAL-c5E | Addgene | RRID: Addgene_161783 |
| pmCherryC1 | Clontech | Cat: 632524, ID: PT3975-5 |
| pCDH | SBI | Cat: CD500B-1, RRID: Addgene_72265 |
| BL21 star (DE3) E. coli | Agilent Technologies | Cat: C601003 |
| HeLa S3 cells | ATCC | ATCC Cat: CCL-2.2, RRID: CVCL_0058 |
| HEK 293T | UC Berkeley Cell Culture Facility | RRID: CVCL_0063 |
| pSpCas9(BB)-2A-GFP vector | Addgene | RRID: Addgene_48138 |
| pMRX-IP-LC3-Halo | Addgene | RRID: Addgene_184899 |
| pMRX-IP-Parkin | Addgene | RRID: Addgene_38248 |
| pMRX-IB-Su9-GFP-Halo | Addgene | RRID: Addgene_184905 |
| pmCherryC1-Rubicon | Addgene | RRID: Addgene_216697 |
| pmCherryC1-Pacer | Addgene | RRID: Addgene_216698 |
| pmCherryC1-Pacer K534N, R623T | Addgene | RRID: Addgene_216699 |
| 2CT-PacerRH | Addgene | RRID: Addgene_216700 |
| 2CT-PacerRH K534N | Addgene | RRID: Addgene_216701 |
| 2CT-PacerRH R623T | Addgene | RRID: Addgene_216702 |
| 2CT-PacerRH R628K | Addgene | RRID: Addgene_216703 |
| 2CT-PacerRH K534N, R623T | Addgene | RRID: Addgene_216704 |
| 2CT-PacerRH K534N,R623T,R628K | Addgene | RRID: Addgene_216705 |
| pMAL-c5e-Rubicon RH | Addgene | RRID: Addgene_216706 |
| pMAL-c5e-Rubicon RH N821K, T911R, K916R | Addgene | RRID: Addgene_216707 |
| 2GT-RAB7A Q67L | Addgene | RRID: Addgene_216708 |
| pCAG-TSF-TBK1 | Addgene | RRID: Addgene_216709 |
| pCG-VSV-G | Addgene | RRID: Addgene_8454 |
| pCMVR8.74 | Addgene | RRID: Addgene_22036 |
| Halo-WIPI2B | Addgene | RRID: Addgene_175025 |
| pCIG2-IRES-Lamp1-mNeon Green | Addgene | RRID: Addgene_219441 |
| pHTN-LC3B | Addgene | RRID: Addgene_219440 |
| pS72 Rab7 rabbit monoclonal | Abcam | Cat: ab302494, RRID: AB_2933985 |
| Anti Rab7 rabbit monoclonal antibody | Abcam | Cat: ab137029, RRID: AB_2629474 |
| Anti Pacer rabbit antibody | Millipore Sigma | Cat: HPA026614, RRID: AB_10602693 |
| Rubicon (D9F7) rabbit monoclonal antibody | Cell Signaling | Cat: #8465, RRID: AB_10891617 |
| Anti-alpha tubulin antibody [DM1A]—Loading Control | Abcam | Cat: ab7291, RRID: AB_2241126 |
| Goat anti-rabbit IgG (H + L) highly Cross-adsorbed secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat: A11034, RRID: AB_2576217 |
| Goat anti-rabbit IgG H&L (HRP) | Abcam | Cat: ab6721, RRID:AB_955447 |
| HSP60 rabbit polyclonal antibody | Sigma-Aldrich | Cat# SAB4501464, RRID: AB_10746162 |
| Anti mCherry chicken polyclonal antibody | Abcam | Cat: ab205402, RRID: AB_2722769 |
| HeLa S3 RUBCN KO clone 15 | This paper | AC: CVCL_D5AG |
| S3 HeLas RUBCN KO + HA-Parkin/pSu9-HaloTag7-mGFP | This paper | AC: CVCL_D5AJ |
| HeLa S3 RUBCN KO/HaloTag7-LC3 | This paper | AC: CVCL_D5AK |
| HeLa S3 RUBCNL KO clone 17 (Pacer KO) | This paper | AC: CVCL_D5AH |
| HeLa S3 RUBCNL KO (Pacer KO)/HA-PRKN/pSu9-HaloTag7-mGFP | This paper | AC: CVCL_D5AL |
| HeLa S3 RUBCNL KO (Pacer KO)/HaloTag7-LC3 | This paper | AC: CVCL_D5AM |
| HeLa S3 PRKN | This paper | AC: CVCL_D5AN |
| HeLa S3 HA-PRKN/HaloTag7-mGFP | This paper | AC: CVCL_D5AP |
| HeLa S3 HaloTag7-LC3 | This paper | AC: CVCL_D5AQ |